A5041497921- Nuclear Receptors and Signaling
- Macrophage Migration Inhibitory Factor
- Adenosine and Purinergic Signaling
- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
- Microfluidic and Bio-sensing Technologies
- Synthesis of Tetrazole Derivatives
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Cells and Metastasis
- Virus-based gene therapy research
- Air Quality and Health Impacts
- Respiratory viral infections research
- Adipokines, Inflammation, and Metabolic Diseases
- Biochemical and Structural Characterization
- Phagocytosis and Immune Regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- Nanoplatforms for cancer theranostics
- Click Chemistry and Applications
- Climate Change and Health Impacts
- Animal Genetics and Reproduction
- Immune Response and Inflammation
Texas A&M University
2016-2025
Vanderbilt University
2010-2021
In-Q-Tel
2010
Snakebite envenoming is a serious and neglected tropical disease that kills ~100,000 people annually. High-quality, genome-enabled comprehensive characterization of toxin genes will facilitate development effective humanized recombinant antivenom. We report de novo near-chromosomal genome assembly Naja naja, the Indian cobra, highly venomous, medically important snake. Our has scaffold N50 223.35 Mb, with 19 scaffolds containing 95% genome. Of 23,248 predicted protein-coding genes, 12,346...
Evidence suggests that targeting cancer cell energy metabolism might be an effective therapeutic approach for selective ablation of malignancies. Using a Seahorse Extracellular Flux Analyzer, we have demonstrated select environmental agents can alter colonic mitochondrial function by increasing respiration-induced proton leak, thereby inducing apoptosis, marker colon risk. To further probe bioenergetics in primary intestinal cells, developed methodology modified and adapted to measure the...
The application of photodynamic therapy (PDT) has aimed at destroying tumor cells and microbiological control in infections. Experimentation vitro with individual elements a biological system enables the study mechanism action provides directions to vivo applications where cancer coexists normal microorganisms, whether opportunistic or not. combined set leads different behavior from each components isolated. In this study, we created simple model investigate effects PDT (1) on coculture...
We report a simple strategy for the creation of lentiviral vectors specific to any desired target cells. SpyTag is inserted into an engineered Sindbis virus envelope protein and displayed on lentivirus surface create virus-SpyTag pseudoparticles (Sind-SpyTag-pp). The serves as covalent anchoring site target-cell-specific cell-binding (CBP) that fused truncated SpyCatcher (SpyCatcherΔ). Target-cell-specific lentiviruses are created by mixing Sind-SpyTag-pp CBP-SpyCatcherΔ in vitro first used...
Abstract PD-L1 is expressed in tumor cells and its interaction with PD-1 plays an important role evading immune surveillance; this can be overcome using or immunotherapy antibodies. This study reports a novel approach for targeting PD-L1. In human breast cancer cell lines 4T1 mouse mammary cells, expression was regulated by the nuclear receptor NR4A1/Sp1 complex bound to proximal germinal center (GC)-rich region of gene promoter. Treatment bis-indole–derived NR4A1 antagonists including...
Summary Dictyostelium discoideum shows chemotaxis towards folic acid (FA) throughout vegetative growth, and cAMP during development. We determined the spatiotemporal localization of cytoskeletal signaling molecules investigated FA-mediated responses in a number mutants to further our understanding core regulatory elements that are crucial for cell migration. Proteins enriched pseudopods also relocalize transiently plasma membrane uniform FA stimulation. In contrast, proteins absent from...
There is evidence that the orphan nuclear receptor 4A1 (NR4A1, Nur77) overexpressed in exhausted CD8 + T cells and regulates PD-L1 tumors. This study investigated effects of potent bis-indole-derived NR4A1 antagonists on reversing T-cell exhaustion downregulating colon tumors/cells. inhibited tumor growth downregulated expression mouse MC-38-derived tumors cells. TILs from MC-38 cell-derived splenic lymphocytes exhibited high levels markers including PD-1, 2B4, TIM3+ TIGIT similar results...
Obesity-associated chronic inflammation, aka meta-inflammation, is a key pathogenic driver for obesity-associated comorbidity. Growth hormone secretagogue receptor (GHSR) known to mediate the effects of nutrient-sensing ghrelin in food intake and fat deposition. We previously reported that global GHSR ablation protects against diet-induced inflammation insulin resistance, but site(s) action mechanism are unknown. Macrophages drivers meta-inflammation. To unravel role macrophages, we...
Journal Article Open access microfluidic device for the study of cell migration during chemotaxis Get Dawit Jowhar, Jowhar Department Biological Sciences, Vanderbilt University, VU Station B #351634, Nashville, TN 37235. Fax: +1 615-343-6707; Tel: 615-936-8907 Search other works by this author on: Oxford Academic Google Scholar Gus Wright, Wright Philip C. Samson, Samson Institute Integrative Biosystems Research and Education, TNDepartment Physics Astronomy, John P. Wikswo, Wikswo Biomedical...
The development of high-quality chromosomally assigned reference genomes constitutes a key feature for understanding genome architecture species and is critical the discovery genetic blueprints traits biological significance. South American camelids serve people in extreme environments are important fiber companion animals worldwide. Despite this, alpaca lags far behind those available other domestic species. Here we produced chromosome-level improved assembly using DNA same female Huacaya...
Glioblastoma multiforme (GBM) is the most common and aggressive malignant brain tumor, with a poor prognosis. Temozolomide (TMZ) represents standard chemotherapy for GBM but has limited efficacy due to targeting hypoxic tumor microenvironment (TME). To address these challenges, we developed dual-gas-releasing, cancer-cell-membrane-camouflaged nanoparticle deliver TMZ. This nanoceria, camouflaged cancer cell membrane (CCM-CeO
ABSTRACT Transmembrane helix 2 (TM2) of the Tar chemoreceptor undergoes an inward piston-like displacement 1 to 3 Å upon binding aspartate. This signal is transmitted kinase-control module via HAMP domain. Within Tar, domain forms a parallel four-helix bundle consisting dimer two amphipathic helices connected by flexible linker. In nuclear magnetic resonance structure archaeal domain, residues corresponding MLLT sequence between Arg-214 at end TM2 and Pro-219 are N-terminal helical...
Abstract A microcompressor is a precision mechanical device that flattens and immobilizes living cells small organisms for optical microscopy, allowing enhanced visualization of sub-cellular structures organelles. We have developed an easily fabricated device, which can be equipped with microfluidics, permitting the addition media or chemicals during observation. This used on both upright inverted microscopes. The apparatus permits micrometer flattening nondestructive immobilization...
Twenty-eight day feeding studies were conducted to evaluate the repeated dose toxicity of NMP, a widely used industrial solvent, in Crl:CD®BR rats and B6C3F1 mice. Groups 5 male female each fed either 0, 2,000, 6,000, 18,000, or 30,000 ppm NMP; similar groups mice 500, 2,500, 7,500, 10,000 ppm. In vivo parameters, hematology clinical chemistry complete pathology evaluations after approximately 28 days.Decrements mean body weight gains, reflecting decreases food consumption efficiency, seen...
Abstract Microfluidic devices can provide unique control over both the chemoattractant gradient and migration environment of cells. Our work incorporates laser-machined micro nanofluidic channels into bulk fused silica cover slip-sized wafers. We have designed “open” chemotaxis that produce passive gradients without an external micropipette system. Since area is unobstructed, cells be easily loaded strategically placed with a standard micropipette. The reusable monolithic glass integral...
Background: Previous trials have displayed augmented intracellular vitamin C concentrations in the leukocytes at 24 h after acute supplementation with 1000 mg calcium ascorbate (CA), compared to ascorbic acid (AA). Objective: The primary objective was evaluate comparative leukocyte accumulation kinetics over 32 following 250 or 500 doses from two sources. Secondary objectives were neutrophil phagocytic function and lymphocyte differentiation between sources of C. Methods: Ninety-three...
Abstract Despite recent improvements in the engineering of viral envelope proteins, it remains a significant challenge to create lentiviral vectors that allow targeted transduction specific cell populations interest. In this study, we developed simple ‘plug and play’ strategy retarget any desired types through vitro covalent modification virions with cell-targeting proteins (CTPs). This exploits disulfide bond-forming protein-peptide pair PDZ1 its pentapeptide ligand (ThrGluPheCysAla,...
Piperlongumine and derivatives are being developed as anticancer agents which act primarily inducers of reactive oxygen species (ROS) in cancer cell lines. Many the activities piperlongumine resemble those observed for bis-indole derived compounds that bind orphan nuclear receptor 4A1 (NR4A1) inverse agonists to inhibit NR4A1-regulated pro-oncogenic pathways genes. In this study we show like other NR4A1 inhibited RKO, SW480 HCT116 colon growth migration invasion induced apoptosis. also...
Microfluidic devices designed for chemotaxis assays were fabricated on fused silica substrates using femtosecond laser micromachining. These have built-in chemical concentration gradient forming structures and are ideally suited establishing passive diffusion gradients over extended periods of time. Multiple structures, with identical or distinct characteristics, can be integrated into a single device, migrating cells directly observed an inverted microscope. In this paper, the design,...
The Tar chemoreceptor−CheA−CheW ternary complex of Escherichia coli is a transmembrane allosteric enzyme in which binding ligands to the periplasmic domain modulates activity CheA kinase. Kinase also affected by reversible methylation four glutamyl residues cytoplasmic receptor. E. contains 553 residues. Residues 549−553 comprise NWETF pentapeptide that binds CheR methyltransferase and CheB methylesterase. crystal structure similar Tsr chemoreceptor predicts 263−289 490−515 form most...
To determine the ability of an intraoperative cell salvage (IOCS) system and a leukocyte reduction filter (LRF) to remove hemangiosarcoma (HSA) cells from canine blood.Cultured HSA were added blood simulate hemorrhage address hemoabdomen ruptured splenic HSA. The blood/HSA mixture was processed through IOCS, followed by LRF processing.Whole 3 healthy dogs combined with cultured cells.The quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), multiparameter flow cytometry,...
Abstract Background Interactions between air pollution and infectious agents are increasingly recognized critical to identify, especially protect vulnerable populations. Pregnancy represents a period for influenza infection exposure, yet interactions during pregnancy remain unclear. Maternal exposure ultrafine particles (UFPs, $$\le$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mo>≤</mml:mo> </mml:math> 100 nm diameter), class of particulate matter ubiquitous in urban...