A5100457257- Genomics and Phylogenetic Studies
- Fermentation and Sensory Analysis
- Gut microbiota and health
- Identification and Quantification in Food
- Aquaculture disease management and microbiota
- interferon and immune responses
- Microbial Community Ecology and Physiology
- Microbial Metabolism and Applications
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Clostridium difficile and Clostridium perfringens research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive biology and impacts on aquatic species
- RNA and protein synthesis mechanisms
- Nuts composition and effects
- Metabolomics and Mass Spectrometry Studies
- Advanced biosensing and bioanalysis techniques
- Bacterial Genetics and Biotechnology
- Probiotics and Fermented Foods
- Tea Polyphenols and Effects
- Catalytic Cross-Coupling Reactions
- Genetic diversity and population structure
- Catalytic C–H Functionalization Methods
- Microbial Natural Products and Biosynthesis
- Photosynthetic Processes and Mechanisms
Huazhong University of Science and Technology
2025
Chinese Academy of Sciences
2009-2024
Shanghai Institute of Materia Medica
2024
Zhejiang Chinese Medical University
2024
Jiangnan University
2015-2023
Microbiology Institute of Shaanxi
2020-2023
Institute of Microbiology
2020-2023
Heilongjiang University of Chinese Medicine
2019-2023
First Affiliated Hospital of Zhengzhou University
2020-2023
Aerospace Information Research Institute
2023
Abstract Currently, predictive translation tuning of regulatory elements to the desired output transcription factor (TF)-based biosensors remains a challenge. The gene expression biosensor system must exhibit appropriate intensity, which is controlled by ribosome-binding site (RBS), achieve fine-tuning its dynamic range (i.e. fold change in between presence and absence inducer) adjusting level TF reporter. However, existing TF-based generally suffer from unpredictable range. Here, we...
Diet is a major driver of the structure and function gut microbiota, which influences host physiology. Alcohol abuse can induce liver disease microbiota dysbiosis. Here, we aim to elucidate whether well-known traditional health food Goji berry targets prevent injury induced by acute alcohol intake. The results showed that supplementation for 14 days alleviated as indicated lowering serum aspartate aminotransferase, alanine pro-inflammatory cytokines, well lipopolysaccharide content in...
Gynogenesis is one of unisexual reproduction modes in vertebrates, and produces all-female individuals with identical genetic background. In sexual the roles primordial germ cells on dimorphism gonadal differentiation have been largely studied, two distinct functional models proposed. However, role remains unknown animals, it also unclear whether animals are common animals. To solve these puzzles, we attempt to utilize gynogenetic superiority polyploid Carassius gibelio create a complete...
Butyrate, one of the key aroma compounds in Luzhou-flavor baijiu, is synthesized through two alternative pathways: butyrate kinase (buk) and butyryl-CoA:acetate CoA-transferase (but). A lack knowledge butyrate-producing microorganisms hinder our ability to understand flavor formation mechanism baijiu. Here, temporal dynamics microbial metabolic profiling fermented grains (FG) was explored via PICRUSt based on 16S rRNA gene sequences. We found Bacilli Bacteroidia were major potential...
Double-stranded DNAs are usually present in the form of linear B-form double-helix with base pairs adenine (A) and thymine (T) or cytosine (C) guanine (G), but G-rich DNA can four-stranded G-quadruplex (G4) structures, which plays important roles transcription, replication, translation protection telomeres. In this study, a RNA recognition motif (RRM)-containing protein, BmLARK, was identified demonstrated to bind G4 structures promoters transcription factor BmPOUM2 other three unidentified...
The effectiveness of chemotherapy is often compromised by physiological barriers and an immunosuppressive tumor microenvironment. Cancer-associated fibroblasts (CAFs) significantly contribute to the reconfiguration extracellular matrix (ECM) suppression immune responses, making them crucial targets for therapeutic intervention. Here, a acidic microenvironment-responsive delivery system that utilizes cell-derived microparticles (MPs) as carriers chemotherapeutic agent doxorubicin (DOX)...
Clostridium inhabiting pit mud (PM) is one of the important bacterial populations for synthesizing flavor compounds Chinese strong-flavor baijiu. The long-term cereal fermentation with sorghum as main raw material creates an environment rich in starch, ethanol, and organic acids (mainly lactic acid). However, genetic factors underpinning Clostridium's adaptation to PM remain poorly understood. Here, we performed comparative genomic analysis between 30 mud-associated (PMA) 100 non-pit (NPMA)...
An efficient cyclization of N-tosyl-aniline tethered allenyl aldehydes and arylboronic acids catalyzed by cationic palladium complex is developed. This annulation reaction provides a convenient process for the synthesis 3,4-cis-1,2,3,4-tetrahydroquinoline derivatives in high yields with excellent diastereoselectivity enantioselectivity.
Abstract Persistence of Bacillus thuringiensis is an important factor in determining the success this product as a pest control agent. In report we present development highly active mosquitocidal formulation with high resistance to UV. LLP29-M19 strain Bt, selected by repeated exposure UV was found be resistant The optimized and methods used were statistically analyzed. Using single-factor experiments it determined that optimal concentration sodium alginate, CaCl 2 hollow glass beads 1.0%,...
A novel Gram-stain-positive, rod-shaped, obligately anaerobic, non-motile, spore-forming and binary fission encapsulated bacterium, designated strain JN500901T, was isolated from a mud cellar which has been continuously used for the fermentation of Chinese strong-flavour baijiu over 100 years. Growth JN500901Toccurred at pH 4.5-8.0 (optimum, 5.0), 20-40 °C (37 °C), 0-2 % (w/v) NaCl 0-10 (v/v) ethanol. The Biolog assay revealed that JN500901T metabolized d-fructose, l-fucose, isomaltulose...
Mature vinegar culture has usually been used as a type of autochthonous starter to rapidly initiate the next batch acetic acid fermentation (AAF) and maintain batch-to-batch uniformity AAF in production traditional cereal vinegar. However, vitality dominance functional microbes starters remain unclear, which hinders further improvement yield production. Here, based on metagenomic (MG), metatranscriptomic (MT), 16S rRNA gene sequencings, 11 bacterial operational taxonomic units (OTUs) with...
The gut microbiota has been implicated in the pathogenesis and progression of sepsis. Akkermansia muciniphila is considered to be a promising probiotic with reduced abundance cecal ligation puncture (CLP)-induced sepsis model, its specific outer membrane protein (Amuc_1100) can partially recapitulate function muciniphila. However, role unclear. This study aimed investigate effect Amuc_1100 on septic rats, thereby improving prognosis acute lung injury (ALI). A total 42 adult Sprague-Dawley...
Abstract Evolutionary trajectory and occurrence history of polyploidy have been extensively studied in plants, but they remain quite elusive vertebrates. Here, we sampled gathered 4,159 specimens polyploid Carassius species complex including 1,336 tetraploids 2,823 hexaploids from a large geographic scale (49 localities) across East Asia, identified huge number 427 diverse haplotypes mitochondrial control region, which 74 with total frequency up to 75.498% were shared by tetraploids....
Abstract Background Loaches of Cobitinae, widely distributed in Eurasian continent, have high economic, ornamental and scientific value. However, the phylogeny Cobitinae fishes within genera or family level remains complex controversial. Up to now, about 60 mitogenomes had been deposited GenBank, but their integrated characteristics were not elaborated. Results In this study, we sequenced analyzed complete a female Cobits macrostigma . Then conducted comparative mitogenome analysis revealed...
Heterotrophic and anaerobic microalgae are of significance in both basic research industrial application. A microalga strain was isolated from a wastewater treatment pond identified as Chlorella sorokiniana Shihira et W. R. Krauss GXNN01 terms morphology, physiology, phylogeny. The grows rapidly heterotrophic or mixotrophic conditions with addition various carbon sources, even conditions. maximum growth rate reached 0.28 d(-1) when using d,l-malate the source, protein content 75.32% cell dry...
Neutrophil elastase (NE) is associated with sepsis occurrence and progression. We hypothesized that the NE inhibitor Sivelestat might modulate abnormal gut microbiota metabolites during sepsis.Sixty Sprague-Dawley (SD) rats were randomly divided into sham control (SC), (CLP), sepsis+Sivelestat (Sive) groups. The rats' survival status was monitored for 24 hours postoperatively, feces collected microbiome non-targeted metabolomics analyses.Sivelestat administration significantly improved of...