A5101847566- Connexins and lens biology
- Amino Acid Enzymes and Metabolism
- Chemical Synthesis and Analysis
- Biochemical effects in animals
- Advanced Glycation End Products research
- Enzyme Structure and Function
- Heat shock proteins research
- Carbohydrate Chemistry and Synthesis
- Peptidase Inhibition and Analysis
- Biopolymer Synthesis and Applications
- Metabolism and Genetic Disorders
- Protein purification and stability
- Protein Structure and Dynamics
- Antimicrobial Peptides and Activities
- Spectroscopy Techniques in Biomedical and Chemical Research
- Glycosylation and Glycoproteins Research
- Origins and Evolution of Life
- HIV Research and Treatment
- vaccines and immunoinformatics approaches
- Click Chemistry and Applications
- Yersinia bacterium, plague, ectoparasites research
- Immune Response and Inflammation
- Chemical Reactions and Isotopes
- Immunotherapy and Immune Responses
- Radiation Effects and Dosimetry
Kyoto University
2012-2021
Kyoto University Research Reactor Institute
2010-2020
National Institute of Technology, Tsuyama College
2018
Rights and Resources
2015
Omuta hospital
2012
Bergen Kommune
2012
Fukuyama City Hospital
2012
Shimadzu (Japan)
1998-2010
Kyushu University of Health and Welfare
2010
Technical University of Denmark
2008
Free amino acid enantiomers in adult rat brain extracts were analyzed as their N,O-pentafluoropropionyl isopropyl derivatives by gas chromatography on a capillary column of Chirasil-L-Val. A peak X, which exhibited the same retention time derivative authentic D-serine, was detected extracts. Electron impact and positive chemical ionization mass spectra X identical to those D-serine. The concentration free D-serine ratio D-serine/total serine estimated be 0.27 0.23 mumol/g wet weight,...
We characterized the primary structure of alpha A-crystallin from lens human eye by detailed analyses amino acid sequence, mass, and stereoisomers, found biologically uncommon D- beta-aspartic (Asp) residues in protein. The stereoconfiguration Asp151 Asp58 old subjects (mean age: 80 years) was inverted to D-isomer, were simultaneously isomerized beta-aspartyl residues, which may occur via a succinimide intermediate. This is thought be first observation stereoinversion acids protein vivo. It...
More than 40 peptides associated with tachyplesin and polyphemusin, which are highly abundant in hemocyte debris of the horseshoe crabs Tachypleus tridentatus Limulus polyphemus, were synthesized. Among these peptides, we found that a novel compound, was called T22 ([Tyr-5,12, Lys-7]polyphemusin II), strongly inhibited human immunodeficiency virus type 1 (HIV-1)-induced cytopathic effect viral antigen expression. Its 50% effective concentration 0.008 micrograms/ml, while its cytotoxic 54...
Mammalian secretory phospholipase A2s (sPLA2s) are classified into several groups according to molecular structure and the localization of intramolecular disulfide bridges. Among them, group IIA sPLA2 has been thought be one key enzymes in pathogenesis inflammatory diseases owing its augmented expression under various conditions. However, a number inbred mouse strains, gene is naturally disrupted by frameshift mutation. Here, we report cloning cDNA encoding novel expressed spleen...
Cataracts are caused by clouding of the eye lens and may lead to partial or total loss vision. The mechanism cataract development, however, is not well understood. It thought that abnormal aggregates proteins form with age, causing clarity development cataract. Lens composed soluble α-, β-, γ-crystallins, as long lived proteins, they undergo post-translational modifications including isomerization, deamidation, oxidation, which induce insolubilization, aggregation, function cataracts....
We are routinely exposed to low frequency noise (LFN; below 0.5 kHz) at moderate levels of 60–70 dB sound pressure level (SPL) generated from various sources in occupational and daily environments. LFN has been reported affect balance humans. However, there is limited information about the influence chronic exposure for balance. In this study, we investigated whether a 70 SPL affects vestibule, which one organs responsible mice. Wild-type ICR mice were 1 month (0.1 high (HFN; 16 distance...
The lens proteins are composed of α-, β-, and γ-crystallins that interact with each other to maintain the transparency refractive power lens. Because crystallins long-lived proteins, they undergo various post-translational modifications including racemization, isomerization, deamidation, oxidation, glycation, truncation. In βB2-crystallin, which is most abundant β-crystallin, deamidation asparagine glutamine residues has been reported. Here, we found aspartyl (Asp) residue at position 4...
<h3>Objective and methods</h3> Dysferlin encoded by <i>DYSF</i> deficiency leads to two main phenotypes, limb girdle muscular dystrophy (LGMD) 2B Miyoshi myopathy. To reveal in detail the mutational clinical features of LGMD2B Japan, we observed 40 Japanese patients 36 families with whom dysferlin mutations were confirmed. <h3>Results conclusions</h3> Three (c.1566C>G, c.2997G>T c.4497delT) relatively more prevalent. The mutation was associated late onset, proximal dominant forms...
Graph code is a linear obtained from codes $C$ and certain bipartite graph G. In this paper, I propose an expansion of the definition to general $l$-partite, give its lower bound minimum distance. also example generalized calculate parameters $[n, k, d]$.
We have studied the ability of human C-reactive protein to modulate immune response in vitro.Whereas native did not induce phagocytic leukocytes chemotax or produce superoxide, treatment purified with neutrophil-derived acid proteases produced substances potent effects on leukocyte function.Close examination primary structure revealed three regions evenly distributed throughout each which contain peptide sequences closely resembling amino sequence immunomodulator tuftsin, Thr-Lys-Pro-Arg.We...
Until relatively recently, it was considered that D-amino acids were excluded from living systems except for the cell wall of microorganisms. However, D-aspartate residues have now been detected in long-lived proteins various tissues elderly humans. Formation induces aggregation and loss function, leading to age-related disorders such as cataracts Alzheimer disease. A recent study used LC-MS analyze isomers Asp precisely without complex purification proteins. identify four (Lα, Lβ, Dβ, Dα)...