A5101607146- Genomics and Phylogenetic Studies
- Metabolomics and Mass Spectrometry Studies
- Fish Ecology and Management Studies
- Identification and Quantification in Food
- Aquaculture disease management and microbiota
- Fish Biology and Ecology Studies
- Computational Drug Discovery Methods
- Genetic diversity and population structure
- Protein Degradation and Inhibitors
- Chromatin Remodeling and Cancer
- Molecular Biology Techniques and Applications
- Insect Resistance and Genetics
- Peptidase Inhibition and Analysis
- Health, Environment, Cognitive Aging
- Cancer-related gene regulation
- Animal Genetics and Reproduction
- Mitochondrial Function and Pathology
- Genetic and Kidney Cyst Diseases
- Listeria monocytogenes in Food Safety
- RNA and protein synthesis mechanisms
- Polyamine Metabolism and Applications
- Amphibian and Reptile Biology
- Fish biology, ecology, and behavior
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
Shanghai Ocean University
2021-2025
Resolve Therapeutics (United States)
2022-2023
Tianjin University of Science and Technology
2023
Collaborative Innovation Centre for Advanced Ship and Deep-Sea Exploration
2020
National Institute for Communicable Disease Control and Prevention
2017
BGI Group (China)
2015
Zhongnan University of Economics and Law
2008
Control over the number of mtDNA molecules per cell appears to be tightly regulated, but mechanisms involved are largely unknown. Reversible alterations in amount occur response stress suggesting that control is stress-related diseases including major depressive disorder (MDD). Using low-coverage sequence data from 10,442 Chinese women compute normalized numbers reads mapping mitochondrial genome as a proxy for mtDNA, we identified two loci contribute levels: one within TFAM gene on...
Poly-γ-glutamic acid (γ-PGA) is biodegradable, water-soluble, environment-friendly, and edible. Consequently, it has a variety of industrial applications. It crucial to control production cost increase output for γ-PGA.Here γ-PGA from sugarcane molasses by Bacillus licheniformis CGMCC NO. 23967 was studied in shake-flasks bioreactors, the results indicate that yield could reach 40.668 g/L 5L stirred tank fermenter. Further study found reached 70.436 g/L, cell growth increased 73.20% 55.44%,...
Listeria is ubiquitous in a variety of environments and can be isolated from wide range animal hosts. Rodents are capable carrying pathogenic bacteria their intestines, such as Listeria, disseminate those pathogens into the natural environment to where human activity occurs. In this study, we investigated occurrence antimicrobial susceptibility spp. wild rodents found China. We collected 341 intestinal fecal samples five different regions China, all representing rodent habitats. The isolates...
Anadromous migration toward riverine tributaries is often challenged by altered environmental cues, food scarcity, and energy demands, sometimes at the expense of life itself. Tenualosa ilisha (Clupeiformes: Dorosomatidae), national fish Bangladesh, an anadromous shad, offers a model for understanding molecular mechanisms migration. To this end, we present chromosome-level genome T. compare its transcriptomic imprints from muscle liver across environments to trace physiological shifts...
The big-eye mandarin fish (Siniperca knerii) is an endemic species of southern China. It belongs to the family Sinipercidae, which closely related well-known North American sunfish Centrarchidae. Determining genome sequence S. knerii would provide a foundation for better examining its genetic diversity and population history. A novel sequenced Sinipercidae also help in comparative study Centrarchidae using Siniperca as reference. Here, we determined 10x Genomics technology next-generation...
Abstract SWI/SNF complexes play an important role in controlling gene expression by remodeling chromatin. SMARCA2 (BRM) and SMARCA4 (BRG1) are the core catalytic subunits of complexes, containing ATPase domain a DNA binding bromodomain. protein is lost some cancers due to nonsense mutations, SMARCA4-deleted cancer cells highly dependent on its paralog for their survival. Therefore, targeting using selective degraders induces synthetic lethality while sparing wild type (WT) normal cells. We...
A combination of short-insert paired-ended and mate-pair libraries large insert sizes is used as a standard method to generate genome assemblies with high contiguity. The third-generation sequencing techniques also are improve the quality assembled genomes. However, both require high-molecular-weight DNA, making use these inappropriate for samples only degraded DNA. An in silico that generates using reference was devised task assembling target Although contiguity completeness genomes were...
Abstract Previous studies have indicated that Cranoglanis bouderius and Pangasianodon hypophthalmus clustered into a sister group. However, there was significant difference in their minimum tolerated temperatures. To reveal the temperature adaptation‐related genes, genome‐wide comparative analysis performed. First, chromosome‐level draft genome of C. constructed this study. The assembly 999.18 Mb size with contig N50 20.47 Mb. Then, an additional 118.98 Gb Hi‐C data applied to assemble...
Abstract Correlophus ciliatus, or the crested gecko, is widely kept as a pet in many countries around world due to its ease care and bred high survival rate. However, there limited number of genomic studies on gecko. In this study, we generated high-quality chromosome-level genome assembly gecko by combining Nanopore, Illumina, Hi-C data. The assemble has size 1.66 Gb, with scaffold N50 109.97 Mb, 99.52% anchored 19 chromosomes. BUSCO analysis indicated gene completeness 90.3% (n=7,480),...
Abstract Rhinogobius similis is distributed in East and Southeast Asia. It an amphidromous species found mostly freshwater sometimes brackish waters. We have obtained a high-resolution assembly of the R. genome using nanopore sequencing, high-throughput chromosome conformation capture (Hi-C), transcriptomic data. The assembled was 890.10 Mb size 40.15% GC content. Including 1373 contigs with contig N50 1.54 Mb, scaffold 41.51 Mb. All were anchored on 22 pairs chromosomes. BUSCO evaluation...
Abstract Objectives American shad ( Alosa sapidissima ) is an important migratory fish under Alosinae and has long been valued for its economic, nutritional cultural attributes. Overfishing barriers across the passage made it vulnerable to sustain. To protect this valuable species, aquaculture action plans have taken though there are no published genetic resources prevailing yet. Here, we reported first de novo assembled annotated transcriptome of A. using blood brain tissues. Data...
Abstract Objectives: American shad ( Alosa sapidissima ) is an important migratory fish under Alosine and has long been valued for its economic, nutritional cultural attributes. Overfishing barriers across the passage made it vulnerable to sustain. To protect this valuable species, aquaculture action plans have taken though there are no published genetic resources prevailing yet. So, here we reported a de novo transcriptome assembly annotation A. from blood brain tissues first time. Data...
Abstract Targeted protein degradation (TPD) is an emerging therapeutic modality with the potential to target previously undruggable targets. However, it has been more challenging identify orally bioavailable TPD molecules due physicochemical properties of large and narrow structure-activity relationship (SAR) compared conventional small molecule inhibitors. In present study, we identified active that selectively potently degrade SMARCA2 induce synthetic lethality in SMARCA4-deficient cancer...
Abstract Siniperca undulata and S. obscura (Centrarchiformes: Sinipercidae) are small Chinese perches, living in creeks streams southern China. While they have sympatric distribution occupy similar macrohabitat, their body sizes ecological niches many differences. Determining the genome sequences of would provide us an essential data set for better understanding genetic makeup differences that may play important roles adaptation to different niches. We determined both using 10× genomics...
目的 化疗耐药是乳腺癌复发转移、治疗效果不理想的主要因素。本文探讨miR-142-3p通过靶向高迁移率族蛋白1（high-mobility group box 1，HMGB1）提高乳腺癌化疗敏感性的分子机制。 方法 实时荧光定量PCR检测人乳腺癌MCF-7细胞及阿霉素耐药株MCF-7/DOX细胞中的miR-142-3p水平；MTT法检测阿霉素（doxorubicin，DOX）处理后各组的增殖情况、流式细胞术检测凋亡率、Western blot检测HMGB1和自噬有关蛋白的表达水平；双荧光素酶报告实验评价miR-142-3p对HMGB1的靶向调控作用。 结果 阿霉素耐药株MCF-7/DOX细胞中的miR-142-3p水平明显下调。miR142-3p的过度表达增强了乳腺癌细胞对阿霉素的敏感性和提高阿霉素诱导的凋亡比率。HMGB1是乳腺癌细胞中miR-142-3p的直接功能靶点，HMGB1的过表达可以明显解除由miR-142-3p上调所产生的细胞凋亡和自噬抑制。 结论...
The Laotian shad (Tenualosa thibaudeaui) belongs to the family Clupeidae and is mainly distributed across Lao PRD, Cambodia, northern Thailand. Due overfishing dam reconstruction, on verge of extinction currently listed as vulnerable by IUCN. Nanopore Illumina sequencing data were integrated generate first high-quality genome assembly for T. thibaudeaui. assembled was 638 Mb in size, including 228 scaffolds with a N50 value 16.6 Mb. BUSCO analysis revealed completeness be more than 96%. A...