A5025907960- SARS-CoV-2 and COVID-19 Research
- Bat Biology and Ecology Studies
- Viral Infections and Vectors
- Respiratory viral infections research
- Amphibian and Reptile Biology
- Vector-borne infectious diseases
- COVID-19 Clinical Research Studies
- Mosquito-borne diseases and control
- Inflammasome and immune disorders
- Antimicrobial Peptides and Activities
- Liver Disease Diagnosis and Treatment
- interferon and immune responses
- Long-Term Effects of COVID-19
- Single-cell and spatial transcriptomics
- Health, Environment, Cognitive Aging
- Immune responses and vaccinations
- Zoonotic diseases and public health
- Viral gastroenteritis research and epidemiology
- Evolution and Paleontology Studies
- Immune cells in cancer
- Liver Diseases and Immunity
- Genetic Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Yersinia bacterium, plague, ectoparasites research
Duke-NUS Medical School
2020-2024
SingHealth
2023
Centre for Human Genetics
2015
University of Oxford
2015
The novel coronavirus SARS-CoV-2 is the causative agent of Coronavirus Disease 2019 (COVID-19), a global healthcare and economic catastrophe. Understanding host immune response to still in its infancy. A 382-nt deletion strain lacking ORF8 (Δ382 herein) was isolated Singapore March 2020. Infection with Δ382 associated less severe disease patients, compared infection wild-type SARS-CoV-2. Here, we established Nasal Epithelial cells (NECs) differentiated from healthy nasal-tissue derived stem...
Control over the number of mtDNA molecules per cell appears to be tightly regulated, but mechanisms involved are largely unknown. Reversible alterations in amount occur response stress suggesting that control is stress-related diseases including major depressive disorder (MDD). Using low-coverage sequence data from 10,442 Chinese women compute normalized numbers reads mapping mitochondrial genome as a proxy for mtDNA, we identified two loci contribute levels: one within TFAM gene on...
The dynamics of SARS-CoV-2 infection in COVID-19 patients are highly variable, with a subset demonstrating prolonged virus shedding, which poses significant challenge for disease management and transmission control. In this study, the long-term were investigated using human well-differentiated nasal epithelial cell (NEC) model infection. NECs observed to release onto apical surface up 28 days postinfection (dpi), further corroborated by viral antigen staining. Single-cell transcriptome...
The emergence of novel betacoronaviruses has posed significant financial and human health burdens, necessitating the development appropriate tools to combat future outbreaks. In this study, we have characterized a cell line, IGROV-1, as robust tool detect, propagate, titrate SARS-CoV-2 HCoV-OC43. IGROV-1 cells can be used for serological assays, antiviral drug testing, isolating variants from patient samples. Using time-course transcriptomics, confirmed that exhibit innate immune response...
Due to the paucity of longitudinal molecular studies COVID-19, particularly those covering early stages infection (Days 1-8 symptom onset), our understanding host response over disease course is limited. We perform single cell RNA-seq on 286 blood samples from 108 age- and sex-matched COVID-19 patients, including 73 with samples. examine discrete subtypes continuous states longitudinally, we identify upregulation type I IFN-stimulated genes (ISGs) as predominant signature subsequent...
Inflammasome is linked to many inflammatory diseases, including COVID-19 and autoimmune liver diseases. While severe was reported exacerbate failure, we report a fatal acute-on-chronic failure (ACLF) in stable primary biliary cholangitis-autoimmune hepatitis overlap syndrome patient triggered by mild infection. Postmortem biopsy showed sparse SARS-CoV-2-infected macrophages with extensive ASC (apoptosis-associated speck-like protein containing CARD) speck-positive hepatocytes, correlating...