A5017291420- Aquaculture disease management and microbiota
- Fish Biology and Ecology Studies
- Identification and Quantification in Food
- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
- Fish Ecology and Management Studies
- Environmental Toxicology and Ecotoxicology
- Heat shock proteins research
- Effects and risks of endocrine disrupting chemicals
- Animal Genetics and Reproduction
- Physiological and biochemical adaptations
- Genomics, phytochemicals, and oxidative stress
- Aquaculture Nutrition and Growth
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Microplastics and Plastic Pollution
- Genetic and phenotypic traits in livestock
- Vitamin C and Antioxidants Research
- Advanced biosensing and bioanalysis techniques
- Paraoxonase enzyme and polymorphisms
- Meat and Animal Product Quality
- Selenium in Biological Systems
- Crustacean biology and ecology
- Autophagy in Disease and Therapy
- Polyomavirus and related diseases
- Per- and polyfluoroalkyl substances research
South China Agricultural University
2015-2024
University of California Davis Medical Center
1996
Abstract Pure bacterial cultures were isolated from diseased snakeheads, Channa maculata (Lacepède), suffering high mortality in a farm Zhongshan, southern China. Three isolates, namely ZS20100725, ZS20100725‐1 and ZS20100725‐2, identified as Aeromonas schubertii . All the isolates showed 16S rRNA sequence similarities with A. The exhibited strong virulence to snakeheads experimental challenges LD 50 ranging between 1.4 × 10 4 6.4 6 CFU g −1 Two of positive for haemolysin, elastase, lipase...
Contemporary improvements in the type II clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system offer a convenient way for genome editing zebrafish. However, low efficiencies of and germline transmission require time-intensive laborious screening work. Here, we reported method based on vitro oocyte storage by injecting oocytes advance incubating them medium to significantly improve fertilization (IVF) Compared conventional methods, prior...
Cyprinid herpesvirus 2 (CyHV-2) has caused great economic loss to the crucian carp breeding industry. Upon viral stimulation, eukaryotic cells generally activate expression of anti-oxidative genes maintain intracellular oxidative balance and resist infection. Here, reactive oxygen species (ROS) levels in CyHV-2-infected were monitored show that CyHV-2 induced increase ROS during early infection, excessive accumulation was ameliorated late which accompanied by activated related Nrf2 signaling...
The transcriptional transactivator (Tas) of simian foamy virus type 1 strongly augments gene expression directed by both the promoter in viral long terminal repeat and newly discovered internal located within env gene. A region 121 bp, immediately 5' to TATA box promoter, is required for transactivation Tas. present study aimed identify precise Tas-responsive target(s) this determine role Tas regulation. By analysis clustered-site mutations hybrid promoters transient assays murine cells, two...
Koumine is a component of the Chinese medicinal herb Gelsemium elegans and toxic to vertebrates. We used ciliate Tetrahymena thermophila as model evaluate effects this indole alkaloid in eukaryotic microorganisms. inhibited T. growth viability dose-dependent manner. Moreover, drug produced oxidative stress cells expressions antioxidant enzymes were significantly elevated at high koumine levels (p < 0.05). also caused significant apoptosis 0.05) induced DNA damage Mitophagic vacuoles present...
Haemulidae species are morphologically diversified fishes with wondrous and changeable coloration. These prevalent in global tropical, subtropical, temperate inshore reef areas. Previous morphological classification within some related families was problematic, no comprehensive molecular evaluation conducted on these groups. In this study, we revealed the first phylogenetic analysis involving representative relevant (Nemipteridae Teraponidae). The performed using both mitochondrial DNA (16S...
The great albino northern snakehead, Channa argus, is one of the most important economical fish in China. In present study, cDNA encoding heat shock protein 60 (HSP60) was cloned and characterized. 2462 bp, containing an open reading frame (ORF) a 575-amino-acids polypeptide 61.10 kDa (theoretical isoelectric point [pI]: 5.66). BLAST analysis showed that AcaHSP60 highly similar with other HSP60s, three conserved amino acid blocks characteristic motifs or domains defined as HSP60 family...
In China, the Cranoglanis bouderius is classified as a national class II-protected animal. The development of C. populations has been affected by variety factors over past few decades, with severe declines occurring. Considering likelihood continued population in future, it critical to investigate currently unknown characteristics gonadal differentiation and sex-related genes for conservation. this study, Illumina sequencing platform was used sequence transcriptome identify pathways related...
Relationships of two species Channa argus (northern snakeheads) named "Bicolor type" and "White type", as well other six were investigated based on their partial sequence mitochondrial DNA 16S rRNA genes in the present study. For family, average genetic distance was 0.0863 with inter-species ranged from 0.0173 to 0.1384, intra-species genus channa estimated 0.00273 (range: 0.0019 0.0038). C. species, mean pair-wise between northern snakehead 0.00218, which within interval for indicating that...
Abstract Previous studies have indicated that Cranoglanis bouderius and Pangasianodon hypophthalmus clustered into a sister group. However, there was significant difference in their minimum tolerated temperatures. To reveal the temperature adaptation‐related genes, genome‐wide comparative analysis performed. First, chromosome‐level draft genome of C. constructed this study. The assembly 999.18 Mb size with contig N50 20.47 Mb. Then, an additional 118.98 Gb Hi‐C data applied to assemble...
CYP1A is the most commonly used biomarker and transgenic fish which carrying a cyp1a promoter to drive reporter gene can be as reliable way monitor dioxin/dioxin-like compounds (DLCs) in environment. Here, we cloned of Gambusia affinis this showed stronger transcriptional activity than that zebrafish. Then, Tg(GAcyp1a:eGFP/Luc) zebrafish line was first constructed with G. driving eGFP expression using meganuclease I-SceI mediated transgenesis technology. The larvae at 72 h post-fertilization...
As an unclassified species, white-type Channa argus has existed for many years. In the present study, relationships and genetic diversity in two colour morphs of northern snakehead were determined by analysis mitochondrial 12S rRNA gene, as well other species. The results sequence showed that average distance was 0.046 with interspecies ranging from 0.000 to 0.130; estimated 0.003 (range: 0.000–0.006). For argus, mean pair-wise between them also 0.003. Moreover, molecular phylogenetic tree...
To reveal the evolutionary relationship of three mitten crabs (Eriocheir sinensis, E. hepuensis, and japonica), complete mitogenomes nuclear 5S rDNA sequences were analyzed. Sequencing revealed that analyzed shared conserved organization coding non-coding regions but genetic variation was identified. Among distinct tandem repeats identified in mitochondrial D-loop region. The gene (5S rDNA) sequence highly across species, whereas non-transcribed spacer (NTS) region exhibit high levels...
White type Channa argus, which has the common name of great northern snakehead, an uncertain classification for many years. In present study, mitochondrial COI gene been used to analyze relationships and genetic diversity nine kinds Channa. For Chnannidae family, average inter-species distance was 0.156 (ranged: 0.000–0.245). And intra-species distances were ranged from 0.000 0.002. haplotypes two-colour morphs mean pair-wise estimated as 0.0009. This value within interval, indicating that...