A5103181671- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Aquaculture disease management and microbiota
- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Antimicrobial Peptides and Activities
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Lipid metabolism and biosynthesis
- Nerve injury and regeneration
- Spectroscopy and Quantum Chemical Studies
- Photoacoustic and Ultrasonic Imaging
- RNA Interference and Gene Delivery
- Regional Development and Environment
- Advanced Glycation End Products research
- Microplastics and Plastic Pollution
- Macrophage Migration Inhibitory Factor
- Microbial metabolism and enzyme function
- Land Use and Ecosystem Services
- Heat shock proteins research
- TGF-β signaling in diseases
- Probiotics and Fermented Foods
Third Affiliated Hospital of Southern Medical University
2024
South China Agricultural University
2015-2024
South China University of Technology
2024
Guizhou University of Finance and Economics
2023
Institute of Economics
2022
ORCID
2020
Key Laboratory of Guangdong Province
2011
Ludwig-Maximilians-Universität München
1994
Abstract J‐aggregation brings intriguing optical and electronic properties to molecular dyes significantly expands their applicability across diverse domains, yet the challenge for rationally designing J‐aggregating persists. Herein, we developed a large number of from scratch by progressively refining structure common heptamethine cyanine. J‐aggregates with sharp spectral bands (full‐width at half‐maximum≤38 nm) are attained introducing branched featuring benzyl trifluoroacetyl group...
The process of transgenesis involves the introduction a foreign gene, transgene, into genome an animal. Gene transfer by pronuclear microinjection (PNI) is predominant method used to produce transgenic animals. However, this technique does not always result in germline offspring and has low success rate for livestock. Alternate approaches, such as somatic cell nuclear using fibroblasts, do show increase efficiency compared PNI, while viral-based hampered issues regarding transgene size...
Abstract J‐aggregation brings intriguing optical and electronic properties to molecular dyes significantly expands their applicability across diverse domains, yet the challenge for rationally designing J‐aggregating persists. Herein, we developed a large number of from scratch by progressively refining structure common heptamethine cyanine. J‐aggregates with sharp spectral bands (full‐width at half‐maximum≤38 nm) are attained introducing branched featuring benzyl trifluoroacetyl group...
Abstract Piglets cloned by somatic cell nuclear transfer (SCNT) show a high incidence of malformations and death rate during the perinatal period. To investigate underlying mechanisms for abnormal development pig fetuses, we compared body weight, amniotic fluid (AF) metabolome, placental transcriptome between SCNT‐ artificial insemination (AI)‐derived fetuses. Results showed that weight SCNT fetuses was significantly lower than AI The identified differential metabolites two groups AF were...
Xist is an X-linked gene responsible for cis induction of X chromosome inactivation. Studies have indicated that abnormally activated in the active cloned mouse embryos due to loss maternal Xist-repressing imprint following enucleation during somatic cell nuclear transfer (SCNT). Inhibition expression by injecting small interfering RNA (siRNA) has been shown enhance vivo developmental efficiency male more than 10-fold. The purpose this study was investigate whether a similar procedure can be...
Abstract The salivary glands of animals have great potential to act as powerful bioreactors produce human therapeutic proteins. Human nerve growth factor (hNGF) is an important pharmaceutical protein that clinically effective in the treatment many neuronal and non-neuronal diseases. In this study, we generated 18 transgenic (TG) founder mice each carrying a gland specific promoter-driven hNGF transgene. A TG mouse line secreting high levels its saliva (1.36 μg/mL) was selected. successfully...
Xist is an X-linked ribonucleic acid (RNA) gene responsible for the cis induction of X chromosome inactivation (XCI). In cloned mammalian embryos, ectopically activated at morula to blastocyst stage on that supposed be active, thus resulting in abnormal XCI. Suppression erroneous expression by injecting small interfering RNA (siRNA) remarkably increased developmental efficiency male mouse embryos approximately 10-fold. However, injection anti-Xist siRNA resulted only a slight increase...
As a member of the G0/G1 switch genes, gene 2 (G0S2) is related to many regulatory processes in human and mouse. For example, it interacts directly with adipose triglyceride lipase active its hydrolysis activities. In this study, G0S2 cDNA chicken (522 bp), zebra finch (420 sparrow (417 pigeon Bengalese (416 bp) were cloned, each them was encoded as protein 99 amino acids. The expression mRNA determined by real-time reverse-transcription PCR analysis 20 tested tissues 21- 91-d-old chickens....
The great albino northern snakehead, Channa argus, is one of the most important economical fish in China. In present study, cDNA encoding heat shock protein 60 (HSP60) was cloned and characterized. 2462 bp, containing an open reading frame (ORF) a 575-amino-acids polypeptide 61.10 kDa (theoretical isoelectric point [pI]: 5.66). BLAST analysis showed that AcaHSP60 highly similar with other HSP60s, three conserved amino acid blocks characteristic motifs or domains defined as HSP60 family...
然而， 在农地大规模流转的背景下， 仅有少数研究关注了农地流转对农户化肥投入影
Turtles grow slowly and have a long lifespan. Ultrastructural studies of the pituitary gland in Reeves’ turtle (Chinemys reevesii) revealed that species possesses higher nucleoplasmic ratio fewer secretory granules growth hormone (GH) cells than other animal summer winter. C. reevesii GH gene was cloned species-specific similarities differences were investigated. The full sequence contains 8517 base pairs (bp), comprising five exons four introns. Intron 1 found to be much longer species....
Farm animal salivary glands hold great potential as efficient bioreactors for production of human therapeutic proteins. Nerve growth factor (NGF) is naturally expressed in and has been approved clinical treatment. This study aims to employ transgenic (TG) pig gland synthesis NGF (hNGF). hNGF-TG pigs were generated by cloning combination with piggyBac transposon-mediated gene transfer. These specifically hNGF protein their secreted it at high levels into saliva. Surgical nonsurgical...
The quality of in vitro matured oocytes is inferior to that vivo oocytes, which translates low developmental capacity embryos derived from oocytes. potential usually impaired due oxidative stress. Stromal cell-derived factor-l (SDF1) can reduce stress and inhibit apoptosis. aim this study was investigate the effects SDF1 supplementation during pig oocyte maturation (IVM) on subsequent embryo development, explore acting mechanisms We found IVM medium containing 20 ng/mL improved rate as well...
Previously we successfully produced a group of EGFP-expressing founder transgenic pigs by newly developed efficient and simple pig transgenesis method based on cytoplasmic injection piggyBac plasmids. In this study, investigated the growth reproduction performance characterized transgene insertion, transmission, expression patterns in generated transposition. Results showed that has no injurious effect pigs. Multiple copies monogenic EGFP were inserted at noncoding sequences host genome,...
Human sarcolectin is known as growth promoter and interferon-alpha/beta antagonist. Besides N-acetylneuraminic acid-dependent cell agglutination it also binds to a macrophage migration inhibitory factor (MIF). Several types of negatively charged carbohydrates interfere with this binding, indicating importance cluster. Since human serum albumin that has very similar properties in gel electrophoretic analysis can bind comparatively reduced extent, are compared biochemically immunologically....
Abhydrolase domain containing 5 gene (ABHD5), also known as comparative identification 58 (CGI-58), is a member of the α/β-hydrolase family protein cofactor ATGL stimulating its triacylglycerol hydrolase activity. In this study, we aim to characterize expression and variations ABHD5 study their functions in chicken fat metabolism. We compared level various tissues under different nutrition conditions, identified ABHD5, associated them with production traits an F2 resource population...
Abstract About 70% of all antibiotics produced in the world are used farm animal industry. The massive usage during production has caused rapid development antibiotic resistance bacteria, which poses a serious risk to human and livestock health when treating bacterial infections. Protegrin-1 (PG-1) is potent antimicrobial peptide (AMP). It was initially identified pig leukocytes with broad-spectrum antibacterial antiviral activity, low rate inducing resistance. To develop genetic approach...