Abstract Background Prostate cancer (PCa) remains the second leading cause of deaths due to in United States men. The aim this study was perform an integrative epigenetic analysis prostate adenocarcinoma explore abnormalities involved development and progression adenocarcinoma. key DNA methylation-driven genes were also identified. Methods Methylation RNA-seq data downloaded for Cancer Genome Atlas (TCGA). gene expression from TCGA incorporated analyzed using MethylMix package. Gene...

The effective treatment of urethral stricture remains a medical problem. use proinflammatory cytokines as stimuli to improve the reparative efficacy mesenchymal stem cells (MSCs) towards damaged tissues represents an evolving field investigation. However, therapeutic benefits this strategy in remain unknown. Here, we enriched exosomes derived from human umbilical cord-derived MSCs pretreated with or without tumor necrosis factor alpha (TNF-α) evaluate their effects vivo model TGFβ1-induced...

Purpose: To investigate the risk factors for progression to castration-resistant prostate cancer (CRPC) in metastatic (mPCa) patients who underwent androgen deprivation therapy (ADT). Methods: We analyzed 216 with mPCa ADT between January 2006 and December 2015 at First Affiliated Hospital of Fujian Medical University. Univariate multivariate Cox regression analysis were used explore CRPC. Kaplan-Meier log-rank test evaluate difference progression-free survival (PFS). Results: A total 121...

Bladder cancer (BCa) is one of the important tumors that have been proven to be treatable with immunotherapy. This study aims identify and validate a molecular prognostic index BCa based on immunogenomic landscape analysis.The genome atlas (TCGA) database immunology analysis portal (ImmPort) were used identified differentially expressed immune-related genes (IRGs). Prognostic IRGs screened protein-protein interaction (PPI) network was constructed. Multivariate Cox performed develop BCa....

Abstract Objective To develop a computed tomography (CT) radiomics-based interpretable machine learning (ML) model to preoperatively predict human epidermal growth factor receptor 2 (HER2) status in bladder cancer (BCa) with multicenter validation. Methods In this retrospective study, 207 patients pathologically confirmed BCa were enrolled and divided into the training set ( n = 154) test 53). Least absolute shrinkage selection operator (LASSO) regression was used identify most...

Talin-1 functions to regulate cell–cell adhesion, and its altered expression was reported be associated with human carcinogenesis. A total of 280 tissue specimens from prostate cancer (PCa) patients who underwent radical prostatectomy, 75 cases benign prostatic hyperplasia (BPH) tissue, 6 normal were collected for construction microarray subsequently subjected immunohistochemical staining expression. significantly higher in PCa than both BPH tissues (P <0.001). preoperative prostate-specific...

Objective:The purpose of this study was to evaluate the efficacy and feasibility R.E.N.A.L. Nephrometry Score postoperatively predict high-grade clear-cell renal carcinoma (ccRCC). Methods:The included 288 patients diagnosed with ccRCC who had complete CT/CTA data Scores underwent surgery at our center between January 2012 December 2015.The relationship pathological grade masses evaluated.Results: Univariate analysis indicated that diagnostic modality, cystic necrosis, enlargement regional...

Prostate cancer (PCa) is one of the most common malignant tumors worldwide. Accumulating evidence has suggested that circular RNAs (circRNAs) are involved in development and progression various cancers, they show great potential as novel biomarkers. However, underlying mechanisms specific functions circRNAs PCa remain unknown. Here, we aimed to identify with roles from expression profile.We used data downloaded Gene Expression Omnibus were differentially expressed between samples adjacent...

This study aimed to identify factors predicting the recoverability of renal function after pyeloplasty in adult patients with ureteropelvic junction obstruction.We retrospectively reviewed 138 adults unilateral obstruction-induced hydronephrosis and who underwent Anderson-Hynes dismembered from January 2013 2016. Hydronephrosis was classified preoperatively according Society for Fetal Urology (SFU) grading system. All Doppler ultrasonography, excretory urography, computed tomography,...

Connexin 43, a gap junction protein, coordinates cell-to-cell communication and adhesion. Altered 43 expression associated with cancer development progression. In this study, we assessed for association clinicopathological features biochemical recurrence of prostate after radical prostatectomy. Pathological specimens were collected from 243 patients who underwent prostatectomy 60 benign prostatic hyperplasia (BPH) to construct tissue microarrays immunohistochemical analysis expression....

Background: This study compared magnetic resonance imaging-guided biopsy (MRI-GB) and transrectal ultrasound guided (TRUS-GB) with the final histology of radical prostatectomy (RP) specimen. Methods: Our subjects were 229 patients prostate cancer (PCa), proven histopathologically using MRI-GB or TRUS-GB, who underwent RP at our center between December 2015 2016. The main group included 92 control 137 12-core TRUS-GB. Histological findings for specimens those from biopsies. We also evaluated...

Abstract Lymph node metastasis is one of the most important independent risk factors that can negatively affect prognosis prostate cancer (PCa); however, exact mechanisms have not been well studied. This study aims to better understand underlying mechanism lymph in PCa by bioinformatics analysis. We analysed a total 367 cases from genome atlas database and performed weighted gene co‐expression network analysis explore some modules related metastasis. Gene Ontology pathway enrichment were...

M2-tumor-associated macrophages (TAMs) work as a promoter in the processes of bone metastases, chemotherapy resistance, and castration resistance prostate cancer (PCa), but how M2-TAMs affect PCa has not been fully understood. In this study, we analyzed proportion tumor-infiltrating immune cells using CIBERSORT algorithm, based on samples from Cancer Genome Atlas database. Then performed weighted gene co-expression network analysis to examine modules concerning infiltrated M2-TAMs. Gene...

Abstract Expression of kinesin family member 18B (KIF18B), an ATPase with key roles in cell division, is deregulated many cancers, but its involvement prostate cancer (PCa) unclear. Here, we investigated the expression and function KIF18B human PCa specimens lines using bioinformatics analyses, immunohistochemical immunofluorescence microscopy, RT-qPCR western blot analyses. was overexpressed compared paracancerous tissues associated poorer disease-free survival. In vitro, knockdown promoted...

Objective: To explore risk factors of infectious complications following transrectal ultrasound-guided prostate biopsy (TRUSPB). Methods: We retrospectively analyzed 1,203 patients with suspected cancer who underwent TRUSPB at our center between December 2012 and 2016. Demographics, clinical characteristics, data regarding were collected, then univariate multivariate logistic regression analyses used to identify independent for after biopsy. Results: Multivariate analysis demonstrated that...

Objective: To evaluate the efficacy and safety of bipolar transurethral enucleation resection prostate (B-TUERP) versus (B-TURP) in treatment prostates larger than 60g. Material Methods: Clinical data for 270 BPH patients who underwent B-TUERP 204 B-TURP from May 2007 to 2013 at our center were retrospectively analyzed. Outcome measures included operative time, decreased hemoglobin level, total specific antigen (TPSA), International Prostate Symptom Score (IPSS), maximal urinary flow rate...

Background. To investigate the factors associated with occurrence of and recovery from stress urinary incontinence (SUI) after plasmakinetic enucleation prostate (PKEP). Materials Methods. This retrospective study enrolled 1,288 patients benign prostatic hyperplasia treated January 2008 to 2015, collecting demographics clinical parameters. SUI was defined as a patient complaint involuntary urine leak, including or mixed incontinence. Logistic regression analysis used SUI. Results. PKEP...

Abstract The significance of actin‐related protein 2/3 complex subunit 4 (ARPC4) expression in bladder cancer, and its potential role the invasion migration cancer cells, has yet to be determined. This study was identify correlation between ARPC4 lymph node metastasis, determine invasive T24 cells. One hundred ninety‐eight tissues 40 normal were examined. Tissue microarrays constructed subjected immunohistochemical stating for ARPC4. Multiple logistic analysis used risk factors associated...

Abstract This study aimed to explore the association between LIM domain kinase 1 (LIMK1) expression in prostate cancer (PCa) tissues with advanced pathological features, lymph node metastases and biochemical recurrence. A total of 279 PCa specimens from patients who underwent radical prostatectomy 50 benign prostatic hyperplasia (BPH) were collected construct tissue microarray, which subjected immunohistochemical staining for LIMK1 subsequently. Logistic Cox regression analysis used evaluate...

Purpose: To examine the effects and mechanism of action fasudil on cytoskeletal polymerization, collagen synthesis, apoptosis in fibroblasts derived from human urethral scar tissue. Materials methods: Fibroblasts treated with or without transforming growth factor β1 (TGF-β1, 10 ng/mL) were incubated (12.5, 25, 50 µmol/L) for 24 hours. Quantitative real-time polymerase chain reaction Western blotting used to determine expression Arp2, Arp3, WASP, WAVE2. Collagen I III protein levels also...