A5004750960- Retinal Diseases and Treatments
- Inflammasome and immune disorders
- interferon and immune responses
- Tryptophan and brain disorders
- Ocular Diseases and Behçet’s Syndrome
- RNA regulation and disease
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Retinal Imaging and Analysis
- CRISPR and Genetic Engineering
- Insect and Arachnid Ecology and Behavior
- MicroRNA in disease regulation
- HIV-related health complications and treatments
- Immune cells in cancer
- Cytomegalovirus and herpesvirus research
- Diabetes, Cardiovascular Risks, and Lipoproteins
- HIV Research and Treatment
- Retinal and Optic Conditions
- Insect Pest Control Strategies
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Insect and Pesticide Research
- Chromosomal and Genetic Variations
Center for Digital Image Evaluation
2017-2023
Yale University
2023
University of Virginia
2020-2021
University of the Virgin Islands
2021
Significance Dry age-related macular degeneration (AMD) affects the vision of millions people worldwide. There is currently no Food and Drug Administration–approved treatment for dry AMD. The inflammasome components NLRP3 ASC have been implicated in pathogenesis We report that fluoxetine, which approved clinical depression, directly binds protein prevents NLRP3-ASC assembly activation. Fluoxetine retinal pigmented epithelium cells an animal model also present evidence from a big data...
Significance Alu elements, comprising more than 10% of the human genome, propagate via retrotransposition. This genomic expansion requires enzymatic activity L1 that reverse transcribes RNA into cDNA in nucleus. We report also undergoes L1-mediated transcription self-priming cytoplasm independent retrotransposition, providing evidence DNA synthesis this cellular compartment. newly discovered shunt molecule replication cycle induces death retinal pigmented epithelium, a hallmark atrophic...
Long interspersed nuclear element-1 (L1)–mediated reverse transcription (RT) of
Detection of microbial products by multiprotein complexes known as inflammasomes is pivotal to host defense against pathogens. Nucleotide-binding domain leucine-rich repeat (NLR) CARD containing 4 (NLRC4) forms an inflammasome in response bacterial products; this requires their detection NLR family apoptosis inhibitory proteins (NAIPs), with which NLRC4 physically associates. However, the mechanisms underlying sterile activation, implicated chronic noninfectious diseases, remain unknown....
Abstract Nonfibrillar amyloid-β oligomers (AβOs) are a major component of drusen, the sub-retinal pigmented epithelium (RPE) extracellular deposits characteristic age-related macular degeneration (AMD), common cause global blindness. We report that AβOs induce RPE degeneration, clinical hallmark geographic atrophy (GA), vision-threatening late stage AMD is currently untreatable. demonstrate activation NLRP3 inflammasome in mouse vivo and expression purinergic ATP receptor P2RX7, an upstream...
Purpose: Azidothymidine (AZT), a nucleoside reverse transcriptase inhibitor, possesses anti-inflammatory and anti-angiogenic activity independent of its ability to inhibit transcriptase. The aim this study was evaluate the efficacy 5′-glucuronyl azidothymidine (GAZT), an antiretrovirally inert hepatic clinical metabolite AZT, in mouse models retinal pigment epithelium (RPE) degeneration choroidal neovascularization (CNV), hallmark features dry wet age-related macular (AMD), respectively....
Innate immune signaling through the NLRP3 inflammasome has been implicated in pathogenesis of Alzheimer's disease (AD), most prevalent form dementia. We previously demonstrated that nucleoside reverse transcriptase inhibitors (NRTIs), drugs approved to treat HIV and hepatitis B infections, also inhibit activation. Here we report humans, NRTI exposure was associated with a significantly lower incidence AD two largest health insurance databases United States. Treatment aged 5xFAD mice (a mouse...
Abstract The atrophic form of age-related macular degeneration (dry AMD) affects nearly 200 million people worldwide. There is no FDA-approved therapy for this disease, which the leading cause irreversible blindness among over 50 years age. Vision loss in dry AMD results from retinal pigmented epithelium (RPE). RPE cell death driven part by accumulation Alu RNAs, are noncoding transcripts a human retrotransposon. RNA induces activating NLRP3-ASC inflammasome. We report that fluoxetine, an...
Ant invasion causes damage exceeding $5 billion annually in North America. Commercial repellents often contain chemical compounds that are potential carcinogens and maybe harmful to human health. In this study, we aimed identify natural products with ant-repelling properties. We tested chalk, cinnamon, ginger powder, lemon juice, rosemary table salt, turmeric powder using a custom ring apparatus designed quantify ant-repellence. discovered cinnamon were the most effective ant of products....