A5062085810- Inflammasome and immune disorders
- interferon and immune responses
- Retinal Diseases and Treatments
- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Ocular Diseases and Behçet’s Syndrome
- Telomeres, Telomerase, and Senescence
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Immunology Research
- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Chromosomal and Genetic Variations
- Cytomegalovirus and herpesvirus research
- Ocular Surface and Contact Lens
- RNA modifications and cancer
- MicroRNA in disease regulation
- DNA Repair Mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ocular Oncology and Treatments
- Cancer-related gene regulation
- Retinal and Optic Conditions
- HIV-related health complications and treatments
- SARS-CoV-2 detection and testing
University of Virginia
2017-2024
Dr. Shroff’s Charity Eye Hospital
2021-2023
Creative Commons
2023
University of the Virgin Islands
2021
Jawaharlal Nehru University
2011-2018
National Institute of Immunology
2015-2017
Lady Hardinge Medical College
2007
Significance Dry age-related macular degeneration (AMD) affects the vision of millions people worldwide. There is currently no Food and Drug Administration–approved treatment for dry AMD. The inflammasome components NLRP3 ASC have been implicated in pathogenesis We report that fluoxetine, which approved clinical depression, directly binds protein prevents NLRP3-ASC assembly activation. Fluoxetine retinal pigmented epithelium cells an animal model also present evidence from a big data...
Significance Alu elements, comprising more than 10% of the human genome, propagate via retrotransposition. This genomic expansion requires enzymatic activity L1 that reverse transcribes RNA into cDNA in nucleus. We report also undergoes L1-mediated transcription self-priming cytoplasm independent retrotransposition, providing evidence DNA synthesis this cellular compartment. newly discovered shunt molecule replication cycle induces death retinal pigmented epithelium, a hallmark atrophic...
Long interspersed nuclear element-1 (L1)–mediated reverse transcription (RT) of
Detection of microbial products by multiprotein complexes known as inflammasomes is pivotal to host defense against pathogens. Nucleotide-binding domain leucine-rich repeat (NLR) CARD containing 4 (NLRC4) forms an inflammasome in response bacterial products; this requires their detection NLR family apoptosis inhibitory proteins (NAIPs), with which NLRC4 physically associates. However, the mechanisms underlying sterile activation, implicated chronic noninfectious diseases, remain unknown....
The tumor microenvironment is characterized by nutrient-deprived conditions in which the cancer cells have to adapt for survival. Serum starvation resembles growth factor deprivation characteristic of poorly vascularized and has aided discovery key regulatory genes microRNAs (miRNAs) that a role oncogenic transformation. We report here miR-874 down-regulates major G1/S phase cyclin, cyclin E1 (CCNE1), during serum starvation. Because adaptation vital subsequent oncogenesis, we tested CCNE1...
Background Measles virus nucleoprotein (N) encapsidates the viral RNA, protects it from endonucleases and forms a specific template for transcription replication. It is most abundant protein during infection. Its C-terminal domain intrinsically disordered imparting flexibility to interact with several cellular partners. Principal Findings In this study, we demonstrate that expression of N within mammalian cells resulted in morphological transitions, nuclear condensation, DNA fragmentation...
Abstract Nonfibrillar amyloid-β oligomers (AβOs) are a major component of drusen, the sub-retinal pigmented epithelium (RPE) extracellular deposits characteristic age-related macular degeneration (AMD), common cause global blindness. We report that AβOs induce RPE degeneration, clinical hallmark geographic atrophy (GA), vision-threatening late stage AMD is currently untreatable. demonstrate activation NLRP3 inflammasome in mouse vivo and expression purinergic ATP receptor P2RX7, an upstream...
Glutathione (GSH) is an abundant natural tripeptide with antioxidant properties. Under different conditions, it can play protective as well pathogenic roles. The redox state of the cell has important role in induction apoptosis. Elevated level glutathione cancer cells provides resistance to a number chemotherapeutic drugs. Inhibition synthesis sensitizes for apoptosis and enhances activity We have selected GSH-binding RNA aptamers by employing vitro selection protocol SELEX. Kd value these...
Among numerous point mutation differences between the SARS-CoV-2 and bat RaTG13 coronavirus, only 12-nucleotide furin cleavage site (FCS) exceeds 3 nucleotides. A BLAST search revealed that a 19 nucleotide portion of genome encompassing is 100% complementary match to codon-optimized proprietary sequence reverse complement human mutS homolog (MSH3). The present in may occur randomly but other possibilities must be considered. Recombination an intermediate host unlikely explanation. Single...
Purpose: Azidothymidine (AZT), a nucleoside reverse transcriptase inhibitor, possesses anti-inflammatory and anti-angiogenic activity independent of its ability to inhibit transcriptase. The aim this study was evaluate the efficacy 5′-glucuronyl azidothymidine (GAZT), an antiretrovirally inert hepatic clinical metabolite AZT, in mouse models retinal pigment epithelium (RPE) degeneration choroidal neovascularization (CNV), hallmark features dry wet age-related macular (AMD), respectively....
The primary eukaryotic single-stranded DNA-binding protein, Replication protein A (RPA), binds to DNA at the sites of damage and recruits apical checkpoint kinase, ATR via its partner ATRIP. It has been demonstrated that absence RPA incapacitates ATR-mediated response. We report in RPA, human 1 (hSSB1) INTS3 form sub-nuclear foci, associate with ATR-ATRIP complex recruit it genomic stress. ATRIP foci formed after depletion are abrogated INTS3, establishing hSSB-INTS3 Depletion homologs...
Here we describe a new, non-human, ex-vivo model (goat eye model) for training surgeons in DMEK surgeons. In wet lab setting, goat eyes were used to obtain pseudo-DMEK graft of 8 mm from the lens capsule that was injected into another with same maneuvers described human DMEK. The pseudo-graft can be easily prepared, stained, loaded, injected, and unfolded reproducing similar eye, except descemetorhexis, which cannot performed. behaves useful experience understand steps early learning curve....
Telomeres are tandem repeat sequences present at chromosome end that synthesized by RNA-protein enzyme called telomerase. The RNA component (TR) serves as template for telomerase reverse transcriptase (TERT) generating telomere repeats. TERT is overexpressed in actively dividing cells including cancerous cells, absent differentiated somatic whereas human (hTR) normal well cancer cells. Telomerase overexpression ensures length maintenance actually provides proliferative advantage to Stable...
The migration of chromosomes during mitosis is mediated primarily by kinesins that bind to the and move along microtubules, exerting pulling pushing forces on centrosomes. We report a DNA replication protein, Sld5, localizes centrosomes, resisting microtubular experienced chromosome congression. In absence centriolar satellites, which normally cluster around are dissipated throughout cytoplasm, resulting in loss their known function recruiting centrosomal pericentrin. observed Sld5-deficient...