A5020451172- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Optical Coherence Tomography Applications
- Retinal and Optic Conditions
- Corneal surgery and disorders
- Retinal Imaging and Analysis
- Inflammasome and immune disorders
- Retinal Development and Disorders
- Thyroid Cancer Diagnosis and Treatment
- Ocular Diseases and Behçet’s Syndrome
- Optical Imaging and Spectroscopy Techniques
- interferon and immune responses
- Cerebrovascular and Carotid Artery Diseases
- Ophthalmology and Visual Impairment Studies
- Advanced Fluorescence Microscopy Techniques
- Ubiquitin and proteasome pathways
- Central Venous Catheters and Hemodialysis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Corneal Surgery and Treatments
- Thyroid Disorders and Treatments
- Lung Cancer Research Studies
- Peripheral Artery Disease Management
- RNA modifications and cancer
- Chromosomal and Genetic Variations
Doheny Eye Institute
2014-2024
University of California, Los Angeles
2008-2024
Sunnybrook Health Science Centre
2020
Health Sciences Centre
2020
University of Toronto
2020
VA Greater Los Angeles Healthcare System
2008-2010
West Los Angeles College
2010
Significance Dry age-related macular degeneration (AMD) affects the vision of millions people worldwide. There is currently no Food and Drug Administration–approved treatment for dry AMD. The inflammasome components NLRP3 ASC have been implicated in pathogenesis We report that fluoxetine, which approved clinical depression, directly binds protein prevents NLRP3-ASC assembly activation. Fluoxetine retinal pigmented epithelium cells an animal model also present evidence from a big data...
Significance Alu elements, comprising more than 10% of the human genome, propagate via retrotransposition. This genomic expansion requires enzymatic activity L1 that reverse transcribes RNA into cDNA in nucleus. We report also undergoes L1-mediated transcription self-priming cytoplasm independent retrotransposition, providing evidence DNA synthesis this cellular compartment. newly discovered shunt molecule replication cycle induces death retinal pigmented epithelium, a hallmark atrophic...
PurposeTo identify baseline risk factors for macular atrophy (MA) development in HARBOR via a longitudinal assessment of monthly spectral-domain (SD)-OCT scans. Previous analyses MA examined data from color fundus photography (CFP) and fluorescein angiography (FA).DesignRetrospective, post hoc analysis SD-OCT images (ClinicalTrials.gov identifier, NCT00891735), phase 3, multicenter, prospective, randomized, double-blind, active treatment–controlled clinical trial.ParticipantsPatients (N =...
Long interspersed nuclear element-1 (L1)–mediated reverse transcription (RT) of
Detection of microbial products by multiprotein complexes known as inflammasomes is pivotal to host defense against pathogens. Nucleotide-binding domain leucine-rich repeat (NLR) CARD containing 4 (NLRC4) forms an inflammasome in response bacterial products; this requires their detection NLR family apoptosis inhibitory proteins (NAIPs), with which NLRC4 physically associates. However, the mechanisms underlying sterile activation, implicated chronic noninfectious diseases, remain unknown....
PurposePrevious studies of macular atrophy (MA) in HARBOR analyzed color fundus photography and fluorescein angiography image data. This study performed a longitudinal assessment monthly spectral-domain (SD) OCT scans to determine MA prevalence, incidence, progression HARBOR.DesignPost hoc analysis SD images from (ClincalTrials.gov identifier, NCT00891735), phase 3 multicenter, prospective, randomized, double-blind, active treatment–controlled clinical trial.ParticipantsPatients (n = 1097)...
Background and Objective The utility of incomplete retinal pigment epithelium (RPE) outer atrophy (iRORA) in intermediate age-related macular degeneration (iAMD) as a clinical endpoint is not yet elucidated. We aim to understand the time iRORA development iAMD. Patients Methods Eyes with iAMD without subjects fellow-eye neovascular AMD (nAMD) were identified from visits between 2010 2019 at tertiary clinic. Spectral Domain Optical Coherence Tomography 512 × 128mm cubes (Cirrus HD-OCT, Zeiss)...
Background: Sunitinib, a multitargeted vascular endothelial growth factor and receptor tyrosine kinase inhibitor, causes hypothyroidism in patients who take it for treatment of cancer. Although the pathophysiologic mechanism is unclear, has been claimed that due to inhibition iodide uptake. Methods: To evaluate pathologic induction hypothyroidism, we studied effect sunitinib on FRTL-5 rat thyroid cells. We measured cell growth, 125I-iodide uptake efflux, TSH (TSH-R), sodium-iodide symporter...
Sunitinib malate (Sutent, Pfizer, Inc.; SU11248) is a selective, multitargeted inhibitor of receptor tyrosine kinases and has been shown to inhibit receptors for VEGF, PDGF, KIT, FLT3, RET. The objective this study was determine the effects sunitinib on signal transduction pathways gene expression iodide-metabolizing proteins in papillary cancer cells with RET/PTC1 rearrangement.We investigated cell growth, pathways, thyroid-specific thyroid (PTC) lines that had rearrangement.Sunitinib...
Abstract Nonfibrillar amyloid-β oligomers (AβOs) are a major component of drusen, the sub-retinal pigmented epithelium (RPE) extracellular deposits characteristic age-related macular degeneration (AMD), common cause global blindness. We report that AβOs induce RPE degeneration, clinical hallmark geographic atrophy (GA), vision-threatening late stage AMD is currently untreatable. demonstrate activation NLRP3 inflammasome in mouse vivo and expression purinergic ATP receptor P2RX7, an upstream...
To compare anterior chamber angle parameters based on the location of Schwalbe line (SL) from 2 spectral domain optical coherence tomography (SD-OCT) instruments and to measure their reproducibility.Forty-two eyes 21 normal, healthy participants underwent imaging inferior irido-corneal with Spectralis Cirrus SD-OCT under tightly controlled low-light conditions. SL-angle opening distance (SL-AOD) SL-trabecular iris space area (SL-TISA) were measured by masked, certified graders at Doheny...
Purpose: To evaluate the reproducibility of anterior chamber angle measurements obtained by Zeiss Visante segment optical coherence tomography (AS-OCT).Methods: Twenty eyes from 20 normal subjects with open angles were studied. The was imaged using AS-OCT. angle-opening distance (AOD 500, AOD 750), trabercular iris space area (TISA TISA 750) and scleral spur (SS angle) at inferior location measured. All underwent imaging in a darkened room (1 foot candles measured eye). Images graded masked...
Purpose: Azidothymidine (AZT), a nucleoside reverse transcriptase inhibitor, possesses anti-inflammatory and anti-angiogenic activity independent of its ability to inhibit transcriptase. The aim this study was evaluate the efficacy 5′-glucuronyl azidothymidine (GAZT), an antiretrovirally inert hepatic clinical metabolite AZT, in mouse models retinal pigment epithelium (RPE) degeneration choroidal neovascularization (CNV), hallmark features dry wet age-related macular (AMD), respectively....
The lack of Na+/I− symporter (NIS) gene expression in some thyroid cancer patients has been a major hurdle that limits the efficacy standard radioactive iodide therapy. molecular mechanism contributes to low NIS is not well understood. Activated stimulated by thyroid-stimulating hormone-mediated cAMP/protein kinase A signaling through upstream enhancer (NUE). cAMP pathway also forskolin. In current work, we studied transcriptional activation normal cells and cells. We identified response...
Objectives: To determine the repeatability and reproducibility of Central Corneal Thickness (CCT) measurements using two different anterior segment imaging modalities, including those obtained with new lens attachments for Cirrus 5000 HD-OCT. Methods: A total 32 eyes from 16 normal volunteers (8 male, 8 female) were enrolled in this prospective study. CCT was measured by same examiner HD-OCT Pentacam HR. The results each method averaged compared t-test analysis. agreement between measurement...
To evaluate corneal epithelial thickness (CET) and (CT) in healthy eyes using spectral domain optical coherence tomography.Thirty-six were imaged the Cirrus high-definition (HD)-optical tomography device. The average CET CT assessed Review Software within predefined concentric ring-shaped zones. Specific regions of (superior, inferior, temporal, nasal, superonasal, inferotemporal, superotemporal, inferonasal) also assessed. difference between zones was compared males females.The 48.3, 47.1,...
We evaluate the repeatability of fluorescence lifetime imaging ophthalmoscopy (FLIO) in normal subjects with mydriasis and explore factors that influence FLIO imaging.Thirty-two healthy participants (63 eyes) were enrolled this prospective study. The Heidelberg Engineering system uses a 473 nm blue laser light emitted is detected two wavelength channels, short long spectral channels (SSC, LSC). mean (τm) values computed for entire scan area as well five regions interest (ROI, 1 × mm) at...