A5063842765- Orthopaedic implants and arthroplasty
- Orthopedic Infections and Treatments
- Total Knee Arthroplasty Outcomes
- Bone and Joint Diseases
- Bone fractures and treatments
- Hip disorders and treatments
- Mesenchymal stem cell research
- Bone Metabolism and Diseases
- Bone Tissue Engineering Materials
- Shoulder Injury and Treatment
- Nerve Injury and Rehabilitation
- Orthopedic Surgery and Rehabilitation
- Cardiovascular Issues in Pregnancy
- Shoulder and Clavicle Injuries
- Spinal Cord Injury Research
- Elbow and Forearm Trauma Treatment
- Immune cells in cancer
- Diversity and Career in Medicine
- Pelvic and Acetabular Injuries
- Dental materials and restorations
- Muscle Physiology and Disorders
- Hydrogen's biological and therapeutic effects
- Atrial Fibrillation Management and Outcomes
- Cutaneous Melanoma Detection and Management
- Hospital Admissions and Outcomes
Florida College
2020-2023
Université Paris Cité
2011-2023
Hôpital Cochin
2013-2023
University of Florida
2019-2023
University of Pennsylvania
2023
Mayo Clinic
2023
Groupe Hospitalier Diaconesses Croix Saint-Simon
2014-2022
Mayo Clinic in Arizona
2022
New York University Langone Orthopedic Hospital
2021
Stanford University
2011-2020
Bone fractures are among the most common orthopaedic problems that affect individuals of all ages. Immediately after injury, activated macrophages dynamically contribute to and regulate an acute inflammatory response involves other cells at injury site, including mesenchymal stem (MSCs). These MSCs work in concert modulate bone healing. In this study, we co-cultured undifferentiated M0, pro-inflammatory M1, anti-inflammatory M2 with primary murine vitro determine cross-talk between polarized...
Bone healing involves complex biological pathways and interactions among various cell types microenvironments. Among them, the monocyte-macrophage-osteoclast line-age mesenchymal stem cell-osteoblast lineage are critical, in addition to an initial inflammatory microenvironment. These cellular induce necessary milieu provide cells for bone regeneration immune modulation. Increasing age is accompanied with a rise basal state of inflammation, potentially impairing osteogenesis.Translational...
Abstract Wear particles generated with use of total joint replacements incite a chronic macrophage‐mediated inflammatory reaction, which leads to implant failure. Macrophage activation may be polarized into two states, an M1 proinflammatory state dominating alternatively activated M2 anti‐inflammatory state. We hypothesized that IL‐4, activator macrophages, could modulate polyethylene (PE) particle‐induced osteolysis in experimental murine model. Four animal groups included (a) calvarial...
Abstract The biological mechanisms leading to periprosthetic osteolysis involve both chemokines and the monocyte/macrophage cell lineage. Whether MCP‐1 plays a major role in macrophage recruitment presence of wear particles is unknown. We tested two hypotheses: (1) that exogenous local delivery induces systematic (2) blockade ligand‐receptor axis decreases ultra high molecular weight polyethylene (UHMWPE) particles. Six groups nude mice were used. used non‐invasive imaging assay osteolysis....
Aging is associated with significant bone loss and delayed fracture healing. NF-κB activation highly correlated inflammatory-associated diseases including infection, wear particle exposure, chronic inflammation during natural aging processes. The critical roles of in both the pro-inflammatory response osteoclast-mediated resorption have been well defined. However, biological effects mesenchymal stem cell (MSC)-mediated formation remain largely unknown. In current study, marrow-MSCs were...
Treatment of critical size bone defects is challenging. Recent studies showed that the cytokine stromal cell-derived factor 1 alpha (SDF-1α) has potential to improve regenerative effect low morphogenetic protein 2 (BMP-2) concentrations. The goal this study was demonstrate combined SDF-1α and BMP-2 on regeneration stem cell recruitment using a femoral defect model. A total 72 mice were randomized six groups. External fixators implanted onto right femur each mouse 3 mm created. Depending...
The modulation of macrophage phenotype from pro-inflammatory (M1) to tissue healing (M2) via exogenous addition interleukin-4 (IL-4) facilitates osteogenesis; however, the molecular mediators underlying this phenomenon remain unknown. This study characterizes IL-4-dependent paracrine crosstalk between macrophages and osteoprogenitors its effect on osteogenesis in vitro. Primary murine M1 were co-cultured with MC3T3 cells (M1-MC3T3) both transwell plates direct co-cultures. To modulate M2,...
Although iliac crest autologous bone graft remains the gold standard for treatment of defects, delayed- and nonunions, arthrodeses, several alternative strategies have been attempted, including use mesenchymal stem cells. Whether cells from osteoblast lineage demonstrate systemic recruitment to an acute defect or fracture, whether these directly participate in healing is controversial. This study tests two hypotheses: (1) that exogenous murine MC3T3-E1 osteoprogenitor with a high propensity...