A5101955677- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Chronic Kidney Disease and Diabetes
- Circular RNAs in diseases
- RNA modifications and cancer
- DNA and Nucleic Acid Chemistry
- Aldose Reductase and Taurine
- Heme Oxygenase-1 and Carbon Monoxide
- RNA Interference and Gene Delivery
- Renal Diseases and Glomerulopathies
- Cancer Genomics and Diagnostics
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Kruppel-like factors research
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Fibroblast Growth Factor Research
- Cancer-related molecular mechanisms research
- Acute Kidney Injury Research
- Genomics, phytochemicals, and oxidative stress
- RNA regulation and disease
- CAR-T cell therapy research
- Autophagy in Disease and Therapy
- Nanocluster Synthesis and Applications
- Prenatal Substance Exposure Effects
University of South China
2016-2024
Chenzhou First People's Hospital
2019-2024
Beijing Normal University
2022-2024
Xiangnan University
2023-2024
Affiliated Hospital of Liaoning University of Traditional Chinese Medicine
2023-2024
Southern Medical University
2014-2023
Nanfang Hospital
2014-2023
Nanjing University
2022
First Hospital of Jilin University
2021
Jilin University
2021
Autosomal-dominant spinocerebellar ataxias constitute a large, heterogeneous group of progressive neurodegenerative diseases with multiple types. To date, classical genetic studies have revealed 31 distinct forms and identified 19 causative genes. Traditional positional cloning strategies, however, limitations for finding genes rare Mendelian disorders. Here, we used combined strategy exome sequencing linkage analysis to identify novel ataxia gene, TGM6. We sequenced the whole four patients...
Nanorod alignment for optical asymmetry The high polarizability of chiral nanoassemblies plasmonic nanoparticles can lead to strong dichroism, but light scattering causes the fraction polarized photons generated, as measured by g-factors, be much lower than that liquid crystals. Lu et al. used supramolecular interactions gold nanorods with human islet amyloid peptides assemble metallic superstructures unusually cholesteric order (see Perspective Nam and Kim). long, straight helices increased...
Abstract Background The success of using glycolytic inhibitors for cancer treatment relies on better understanding the roles each frequently deregulated genes in cancer. This report analyzed involvement a key enzyme, alpha-enolase (ENO1), tumor progression and prognosis human glioma. Methods ENO1 expression levels were examined glioma tissues normal brain (NB) tissues. molecular mechanisms its effects cell growth, migration invasion also explored by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl...
For the emerging amphibian genetic model Xenopus tropicalis targeted gene disruption is dependent on zinc-finger nucleases (ZFNs) or transcription activator-like effector (TALENs), which require either complex design and selection laborious construction. Thus, easy efficient genome editing tools are still highly desirable for this species. Here, we report that RNA-guided Cas9 nuclease resulted in precise all ten X. genes analyzed, with efficiencies above 45% readily up to 100%. Systematic...
Aldo-keto reductase family 1, member B10 (AKR1B10), is known to be significantly induced in the cells of various cancers such as breast cancer. However, mechanisms AKR1B10 promoting tumorigenesis cancer remain unclear. In present study, we demonstrated potential role and mechanism invasion migration cells.The expression level carcinoma, para-carcinoma tissues were detected by immunohistochemical evaluation real-time polymerase chain reaction (RT-PCR), correlationships between...
Abstract Background Aberrant expression of Aldo-Keto reductase family 1 member B10 (AKR1B10) was associated with tumor size and metastasis breast cancer in our published preliminary studies. However, little is known about the detailed function underlying molecular mechanism AKR1B10 pathological process cancer. Methods The relationship between elevated overall survival disease-free patients analyzed by Kaplan–Meier Plotter database. Breast cell lines overexpressing (MCF-7/AKR1B10) knockdown...
Precise genome editing is essential for both basic and translational research. The recently developed CRISPR/Cas9 system can specifically cleave a designated site of target gene to create DNA double-strand break, which triggers cellular repair mechanism either inaccurate non-homologous end joining, or site-specific homologous recombination. Unfortunately, homology-directed (HDR) challenging due its very low efficiency. Herein, we focused on improving the efficiency HDR using combination...
Mitochondrial fragmentation is a crucial mechanism contributing to tubular cell apoptosis during acute kidney injury (AKI). However, the of modulating mitochondrial dynamics AKI remains unclear. Numb multifunction adaptor protein that expressed in renal tubules. The aim present study was evaluate role dysfunction AKI.The expression upregulated both ischemia-reperfusion- and cisplatin-induced AKI. Depletion from proximal tubules (PT-Nb-KO) exacerbated shown as more severe damage higher serum...
The mechanisms underlying fibrogenic responses after injury are not well understood. Epithelial cell cycle arrest in G2/M is a key checkpoint for determining wound-healing leading to either normal proliferation or fibrosis. Here, we identify kidney- and liver-enriched circular RNA, circBNC2, which abundantly expressed renal tubular cells hepatocytes but significantly downregulated acute ischemic toxic insult. Loss of circBNC2 at least partially mediated by upregulation DHX9. Gain-...
Abstract Despite recent technological advancements in cell tumor DNA (ctDNA) mutation detection, challenges persist identifying low-frequency mutations due to inadequate sensitivity and coverage of current procedures. Herein, we introduce a super-sensitivity specificity technique for detecting ctDNA mutations, named HiCASE. The method utilizes PCR-based CRISPR, coupled with the restriction enzyme. In this work, HiCASE focuses on testing series EGFR provide enhanced detection technology...
Brain metastasis (BM) is a leading cause of death in patients with non-small cell lung cancer (NSCLC). EGFR mutations primary NSCLC lesions have been associated sensitivity to tyrosine kinase inhibitor (TKI). Therefore, it has become important understand mutation status BM NSCLC, and its clinical implications. samples 136 from South China, which 15 had paired tumors, were retrospectively analyzed for by amplification refractory system (ARMS). Effect on progression-free survival (PFS) overall...
Mental retardation (MR) is a group of common and complex disabilities affecting the central nervous system appears before period brain developmental maturity. Recently, only 40% genetic MR has been identified, however 60% remains unexplained. In this study, we applied exome sequencing to identify mutation p.R430X in UPF3B gene an pedigree, which was validated by Sanger completely cosegregated within family. encodes protein involved nonsense-mediated mRNA decay (NMD). By real-time...
Aims: The risk factors promoting acute kidney injury (AKI) to chronic disease (CKD) progression remain largely unknown. aim of the present study was investigate whether hyperhomocysteinemia (Hhcy) accelerates development renal fibrosis after AKI. Results: Hhcy aggravated ischemia-reperfusion-induced AKI and subsequent fibrotic lesions characterized by excessive extracellular matrix deposition. Mechanistically, RNA binding protein human antigen R (HuR) bound 3′-untranslated region (3′-UTR)...
Despite the increased interest of visible-light-absorbing compound Hypericin (Hyp) in photodiagnosis, photocatalysis, and photodynamic therapy (PDT) applications, a major obstacle still exists; i.e., photoactivity is diminished due to facile aggregation Hyp aqueous environment that induces excited-state quenching. Herein, we explore property bound DNA G-quadruplex by combining multiple steady-state time-resolved spectroscopy. We find aggregation-induced quenching effect can be successfully...
The abnormal expression of many long non-coding RNAs (lncRNAs) has been reported in the progression various tumors. However, potential biological roles and regulatory mechanisms development colorectal cancer (CRC) have not yet fully elucidated. Therefore, it is crucial to identify that lncRNAs can be used for clinical prevention treatment CRC. In our previous work, we identificated a novel lncRNA, lncRNA-KAT7, found lncRNA-KAT7 CRC tissues was significantly lower than matched normal...
Cyclobutane pyrimidine dimer (CPD) is the most abundant DNA photolesion, and it can be repaired by photolyases based on electron-transfer mechanisms. However, photolyase absent in human body lacks stability for applications. Can one develop natural enzyme mimetics utilizing nanoparticles (termed nanozymes) to mimic repairing damage? Herein, we observe successful reversal of thymine T<>T normal T base TiO2 under UVA irradiation. Time-resolved spectroscopy provides direct evidence that...