A5051714114- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Marine and coastal ecosystems
- Polyamine Metabolism and Applications
- Marine and coastal plant biology
- Insect and Arachnid Ecology and Behavior
- Marine Ecology and Invasive Species
- Bacteriophages and microbial interactions
- Methane Hydrates and Related Phenomena
- Polar Research and Ecology
- Marine Biology and Ecology Research
- Photosynthetic Processes and Mechanisms
- Neurobiology and Insect Physiology Research
- Chromosomal and Genetic Variations
- Advanced Chemical Sensor Technologies
- Plant and animal studies
- Diatoms and Algae Research
- Bacillus and Francisella bacterial research
- Insect and Pesticide Research
- Olfactory and Sensory Function Studies
- Chemical synthesis and alkaloids
- Environmental DNA in Biodiversity Studies
- Redox biology and oxidative stress
- Fungal and yeast genetics research
Università di Camerino
2016-2025
Massachusetts Institute of Technology
2023
Enterprise Ireland
2023
University of California, Irvine
1992
Eni (Italy)
1991
In recent years, an increasing diversity of species has been recognized within the family Francisellaceae. Unfortunately, novel isolates are sometimes misnamed in initial publications or multiple sources propose different nomenclature for genetically highly similar isolates. Thus, unstructured and occasionally incorrect information can lead to confusion scientific community. Historically, detection Francisella tularensis environmental samples challenging due considerable unknown genetic...
In line with the Italian “Farmers-Nature Project” aimed at reducing conflicts between wild predators and livestock farmers, spatiotemporal activity of red fox was monitored in a protected area central Italy over one-year period. parallel, farmers living study were asked to participate an interview about their perception its predatory towards livestock. Analysis data obtained by camera trapping revealed that higher number sightings recorded February March relation search for food, while lower...
Ciliated protists are ideal material for studying the origin and evolution of sex, because their nuclear dimorphism (containing both germline micronucleus somatic macronucleus in same cytoplasm), special sexual processes (conjugation autogamy), high diversity mating-type systems. However, study process is limited to only a few species, due difficulties inducing or observing conjugation. In present study, we investigate conjugation Paramecium multimicronucleatum: (1) three prezygotic...
Wild-type strains of the protozoan ciliate Euplotes collected from different locations on coasts Antarctica, Tierra del Fuego and Arctic were taxonomically identified as morpho-species nobilii , based morphometric phylogenetic analyses. Subsequent studies their sexual interactions revealed that mating combinations Antarctic form stable pairs conjugant cells. These isolated shown to complete mutual gene exchange cross-fertilization. The biological significance this finding was further...
Ciliates represent a morphologically and genetically distinct group of single-celled eukaryotes that segregate germline somatic functions into two types nuclei exhibit complex cytogenetic events during the sexual process conjugation, which is under control so-called "mating type systems". Studying conjugation in ciliates may provide insight our understanding origins evolution sex fertilization. In present work, we studied detail using model species Euplotes vannus, compared these nuclear...
Ciliates form a distinct group of single-celled eukaryotes that host two types nuclei (micro and macronucleus) in the same cytoplasm have special sexual process known as conjugation, which involves mitosis, meiosis, fertilization, nuclear differentiation, development. Due to their high species diversity, ciliates evolved different patterns events during conjugation. In present study, we investigate these detail marine Euplotes raikovi. Our results indicate that: (i) conjugation lasts for...
The triplet nature of the genetic code is considered a universal feature known organisms. However, frequent stop codons at internal mRNA positions in Euplotes ciliates ultimately specify ribosomal frameshifting by one or two nucleotides depending on context, thus posing nontriplet these Here, we sequenced transcriptomes eight species and assessed evolutionary patterns arising frameshift sites. We show that sites are currently accumulating more rapidly drift than they removed weak selection....
ABSTRACT The ciliate Euplotes raikovi produces a family of diffusible signal proteins (pheromones) that function as prototypic growth factors. They may either promote cell growth, by binding to pheromone receptors synthesized the same cells from which they are secreted (autocrine activity), or induce temporary shift stage mating (sexual) one other, conspecific (paracrine activity). In constitutively secreting E r -1, it was first observed expression -1 receptor “p15,” type II membrane...
ABSTRACT A strain of Francisella endociliophora was isolated from a laboratory culture the marine ciliate Euplotes raikovi . Here, we report complete genome sequence bacterial FSC1006 ( Strain Collection, Swedish Defence Research Agency, Umeå, Sweden).
Glutathione peroxidases (GPxs) form a broad family of antioxidant proteins essential for maintaining redox homeostasis in eukaryotic cells. In this study, we used an integrative approach that combines bioinformatics, molecular biology, and biochemistry to investigate the role GPxs reactive oxygen species detoxification unicellular model organism Tetrahymena thermophila. Both phylogenetic mechanistic empirical analyses provided indications about evolutionary relationships among GPXs...
The amino acid sequences of five pheromones, Er-2, Er-3, Er-9, Er-11, and Er-20, secreted by cells different mating types the ciliated protozoa Euplotes raikovi, have been determined automated Edman analyses whole proteins germane fragments. In each case, molecular mass was plasma desorption or laser spectrometry in excellent agreement with calculated values. Where available, were also accord corresponding segments precursor molecules predicted from relevant nucleic sequences. Of five, two...
The high-multiple mating system of Euplotes crassus is known to be controlled by multiple alleles segregating at a single locus and manifesting relationships hierarchical dominance, so that heterozygous cells would produce mating-type substance (pheromone). In strain L-2D, now homozygous the locus, we previously identified two pheromones (Ec-α Ec-1) characterized significant variations in their amino acid sequences structure macronuclear coding genes. this study, genes have been analyzed...
Abstract Nuclear (18S and ITS ) mitochondrial (16S) ribosomal RNA gene sequences were determined from genetically distinct wild‐type strains of Antarctic (nine strains), Fuegian (four Greenland strains) Svalbard (three populations the marine ciliate, E uplotes nobilii , analysed for their nucleotide polymorphisms. A close genetic homogeneity was found within between populations, while more significant levels differentiation detected two Arctic as well these Antarctic/Fuegian ones. The...
Organisms living in polar waters must cope with an extremely stressful environment dominated by freezing temperatures, high oxygen concentrations and UV radiation. To shed light on the genetic mechanisms which marine ciliate, Euplotes nobilii, relies to effectively oxidative stress, attention was focused methionine sulfoxide reductases repair proteins oxidized methionines. A family of four structurally distinct MsrB genes, encoding enzymes specific for reduction methionine-sulfoxide R-forms,...