Resistance to Phytophthora sojae isolate PsMC1 was evaluated in 102 F2∶3 families derived from a cross between the resistant soybean cultivar Wandou 15 and susceptible Williams genotyped using simple sequence repeat (SSR) markers. The segregation ratio of resistant, segregating, phenotypes population suggested that resistance dominant monogenic. Twenty-six polymorphic SSR markers were identified on chromosome 17 (Molecular linkage group D2; MLG D2), which linked gene based bulked analysis...

The objective of this study was to detect the expression MiR-21-5p in non-small-cell lung cancer (NSCLC) tissues, and investigate effect its on progression NSCLC.Real-time fluorescent quantitative PCR used relative 118 NSCLC tumor tissues their adjacent normal tissues. expressions SMAD7, MMP-9, E-cadherin, vimentin proteins were detected by Western blotting or immunohistochemistry. Cell colony formation, scratch, Transwell assays proliferation, migration, invasion ability A549 cells,...

We aimed to investigate the expressions of miR-21 and miR-210 in breast cancer tissue their correlation with clinicopathological features prognosis.A retrospective analysis was performed on 68 patients treated surgically Wuhan General Hospital Guangzhou Military 2014-2015. The adjacent normal were collected from patients. Quantitative real-time PCR (qRT-PCR) used detect expression levels tissues.According qRT-PCR, significantly higher than those (P<0.05), which correlated lymph node...

An emerging bacterial disease with symptoms resembling those of halo blight is threatening mung bean production in China. This study was conducted to investigate the disease’s geographic distribution China using consecutive multiyear field surveys and confirm causative agents’ identity. The were 15 provinces covering seven regions from 2009 2014. survey results revealed that has rapidly spread prevalent three main Chinese regions, which contain more than 90% mung-bean-growing areas To causal...

摘要: 利用来自不同生态区的8个白粉菌菌株对20世纪80年代以来国家审定(认定)品种、近期参加国家区域试验的品系和核心种质等小麦材料进行抗病性评价,同时利用与Pm4a、Pm8和Pm21基因连锁的分子标记检测了相关抗病基因的存在。在148个国家审定品种中有16.9%的品种能够抗多个菌株,其中大多是近10年选育的品种。不同年代审定的品种中感病品种的频率均超过50%。各个小麦生产区抗病品种的频率高低与该地区白粉病的严重性和育种的关注程度有一定关系。在1...

Foliar diseases are common in most maize-producing regions and have caused serious yield reduction China. To evaluate genetic resistance of parental lines actively used maize breeding programs to major foliar diseases, 152 inbred were tested against northern corn leaf blight (NCLB), southern (SCLB), Curvularia spot (CLS), gray (GLS), rust, rust from 2003 2005. A small number exhibited highly resistant reactions but none NCLB, SCLB, CLS, GLS. Although 53.3%, 40.8%, 80.7% the was moderate....

MicroRNAs (miRs) are widely involved in regulating tumor development and progression. miR-758-3p has been reported to suppress the progression of various cancer types, including hepatocellular carcinoma. However, whether a role bladder (BC) not previously reported, was thus investigated present study. It revealed that downregulated BC tissues cell lines. Transfection with mimics suppressed proliferation, migration invasion cells, inhibition had opposite effect. In terms underlying...

Abstract Background Acute respiratory distress syndrome (ARDS) has been a prevalent disease in ICU with mortality of up to 27–45%. A considerable proportion extubated ARDS patients passing spontaneous breathing trial (SBT) still requires reintubation. Methods Lung ultrasonography (LUS) was used predict the success rate extubation. Ninety-two 60-min SBT were included this prospective research. Their clinical characters including LUS, APACHE II, SOFA, CPIS, EVLWI and PaO 2 /FiO collected...

This study amied to investigate the prognostic characteristics of triple negative breast cancer (TNBC) patients by analyzing B cell marker genes based on single-cell and bulk RNA sequencing.

Abstract Mutations in RP2 and RPGR genes are responsible for the X-linked retinitis pigmentosa (XLRP). In this study, we analyzed gene mutations five Han Chinese families with XLRP. An approximately 17Kb large deletion including exon 4 5 of was found an XLRP family. addition, four frameshift three novel c.1059 + 1 G &gt; T, c.2002dupC c.2236_2237del CT, as well a previously reported mutation c.2899delG were detected other families. Our study further expands spectrum RPGR, will be helpful...

We investigated the pharmacokinetic (PK) and pharmacodynamic (PD) parameters of linezolid in patients who had suffered cerebral hemorrhage after lateral ventricular drainage.Ten with drainage stroke-associated pneumonia were given enrolled. Plasma cerebrospinal fluid (CSF) samples taken at appropriate intervals first administration assayed by high-performance liquid chromatography (HPLC). Then, PK estimated, a Monte Carlo simulation was used to calculate probability target attainments (PTAs)...

The tumor microenvironment plays a major role in multiple myelomas (MM). MM-BMSCs (bone marrow mesenchymal stromal cells) can support growth and immune surveillance escape. On the other hand, fibroblast activation protein α, expressed by cancer stroma cells including BMSCs, has been shown to potentiate epithelial cancers suppression.MM-BMSC inhibited proliferation of T (P = 0.0138), promoted senescence < 0.001), consistent with decreased CD28 hTERT expression Treg/Th17 was down-regulated...

Dysregulation of long non‑coding RNA (IncRNA) antisense in the INK4 locus (ANRIL) is associated with risk myocardial infarction (MI). Therefore, present study aimed to determine mechanisms underlying this association, which currently poorly understood, best our knowledge. The current used an vitro ischemia and reperfusion (MI/R) model, H9c2 cardiomyocytes were exposed hypoxia/reoxygenation (H/R), demonstrated that ANRIL expression was downregulated positively regulated sirtuin 1 (SIRT1)...

Nosocomial pneumonia (NP) is a frequent complication among patients with intracerebral hemorrhage (ICH). However, there are currently no pharmacokinetic (PK) and pharmacodynamic (PD) data to guide meropenem dosing in these patients.To investigate the PK/PD properties of whether usual regimens (2-hour infusion, 1 g, every 8 hours) was suitable.A total 11 diagnosis ICH complicated NP were selected emergency internal medicine treated 1-g/2-hours extended infusion model. The plasma...