A5089017054- Vector-borne infectious diseases
- Immunotherapy and Immune Responses
- Viral Infections and Vectors
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Vector-Borne Animal Diseases
- SARS-CoV-2 and COVID-19 Research
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Extracellular vesicles in disease
- Cancer Immunotherapy and Biomarkers
- Cancer, Hypoxia, and Metabolism
- Graphene and Nanomaterials Applications
- Animal Virus Infections Studies
- MXene and MAX Phase Materials
- Plant Parasitism and Resistance
- Advanced Memory and Neural Computing
- Nanoparticle-Based Drug Delivery
- 2D Materials and Applications
- Lung Cancer Research Studies
- Breast Cancer Treatment Studies
- Immune Cell Function and Interaction
- Cancer Cells and Metastasis
- Influenza Virus Research Studies
- Toxin Mechanisms and Immunotoxins
University of Ottawa
2020-2025
Wuhan University of Technology
2024
Ottawa Hospital
2020-2023
Institute of Infection and Immunity
2020-2023
Ottawa Hospital Research Institute
2020-2023
Introduction The incidence of Lyme disease (LD) in Canada and the United States has risen over last decade, nearing 480,000 cases each year. Borrelia burgdorferi sensu lato, causative agent LD, is transmitted to humans through bite an infected tick, resulting flu-like symptoms often a characteristic bull’s-eye rash. In more severe cases, disseminated bacterial infection can cause arthritis, carditis neurological impairments. Currently, no vaccine available for prevention LD humans. Methods...
In recent years, lipid nanoparticles (LNPs) have emerged as a revolutionary technology for vaccine delivery. LNPs serve an integral component of mRNA vaccines by protecting and transporting the payload into host cells. Despite their prominence in vaccines, there remains notable gap our understanding potential application delivery DNA vaccines. this study, we sought to investigate suitability leading LNP formulations plasmid (pDNA). addition, aimed explore key differences properties popular...
Lyme disease (LD), caused by infection with the tick-borne bacteria, Borrelia burgdorferi, is associated a wide array of symptoms in human patients. Variations clinical manifestations are thought to be influenced genetic differences among B. burgdorferi strains. In this study, we evaluated infectivity, tissue bacterial load, pathology, and immunogenicity five strains sensu stricto (297 Ah130, Bb16-54, B31-A3, Bb16-126, JD1) female C3H/HeN mice at three infectious doses (104, 105, 106...
Abstract Natural killer cell‐derived extracellular vesicles (NK‐EVs) have shown promising potential as biotherapeutics for cancer due to their unique attributes cytotoxic nanovesicles against cells and immune‐modulatory activity towards immune cells. However, a biomanufacturing workflow is needed produce clinical‐grade NK‐EVs pre‐clinical clinical applications. This study established novel using closed‐loop hollow‐fibre bioreactor continuously from the clinically relevant NK92‐MI cell line...
The effectiveness of mRNA vaccines largely depends on their lipid nanoparticle (LNP) component. Herein, we investigate the DLin-KC2-DMA (KC2) and SM-102-based LNPs for intramuscular delivery a plasmid encoding B.1.617.2 (Delta) spike fused with CD40 ligand. LNP encapsulation this CD40L-adjuvanted DNA vaccine either formulation drastically enhanced antibody responses, enabling neutralization heterologous Omicron variants. DNA-LNP formulations provided excellent protection from homologous...
The rising prevalence of Lyme disease (LD) in North America and Europe has emerged as a pressing public health concern. Despite the availability veterinary LD vaccines, no vaccine is currently available for human use. Outer surface protein C (OspC) found on outer membrane causative agent,
The nucleoprotein (NP) of type A influenza virus (IAV) is highly conserved across all strains, making it an attractive candidate antigen for universal vaccines. While various studies have explored NP-induced mucosal immunity, here we interrogated the mechanistic differences between intramuscular (IM) and intranasal (IN) delivery a recombinant adenovirus carrying NP fused with bifunctional CD40 ligand. Despite being less effective than IM in inducing systemic cellular immune responses...
RNA-based agents (siRNA, miRNA, and mRNA) can selectively manipulate gene expression/proteins are set to revolutionize a variety of disease treatments. Nanoparticle (NP) platforms have been developed deliver functional mRNA or siRNA inside cells overcome their inherent limitations. Recent studies focused on knock down proteins causing drug resistance technology introduce tumor suppressors. However, cancer needs multitargeted approaches multiple expressions/proteins. In this proof-of-concept...
In this study, we report the basic characteristics of Rhipicephalus simus mitochondrial genome, including structural organization and base composition rRNAs, tRNAs protein-coding genes. The total length mitogenome was 14,929 bp, included 37 genes with a genome structure similar to other ticks.
Abstract RNA-based agents such as siRNA, miRNA, and mRNA can selectively manipulate gene expression/proteins have the potential to revolutionize current therapeutic strategies for various diseases, including cancer. To address poor stability inherent limitations of RNA agents, nanoparticle (NP) platforms been developed deliver functional or siRNA inside cells. Recent studies focused on either knock down proteins causing drug resistance technology introduce tumor suppressors. However, complex...