Background As one of the main pathogens causing tea anthracnose disease, Colletotrichum gloeosporioides has brought immeasurable impact on sustainable development agriculture. Given adverse effects chemical pesticides to environment and human health, biological control been a focus research this pathogen. Bacillus altitudinis GS-16, which was isolated from healthy leaves, had exhibited strong antagonistic activity against disease. Methods The antifungal mechanism endophytic bacterium GS-16...

During a field study in the Baili Azalea Forest Area Guizhou Province, China (27°12′N, 105°48′E) between May and July 2023, symptoms of leaf spot were observed on Rhododendron latoucheae Franch. The incidence R. leaves was about 12% 1 hm2, significantly reducing their ornamental economic value. affected bore irregular, grey-white lesions with distinct dark brown borders, accompanied by black conidiomata. To isolate pathogen, 15 symptomatic collected from 10 plants. Conidiomata present...

<title>Abstract</title> Ovary is a vital and dynamic reproductive organ. Ovarian tissue cryopreservation (OTC) plays role in female fertility preservation, especially for adolescent cancer patients. Yet, the sensitive cell populations cryoinjury molecular mechanisms underlying remain elusive. This study employs single-cell RNA sequencing spatial transcriptomics to dissect impacts of temperature stress cryoprotectant toxicity on human ovarian cortex. The spatiotemporal characteristics eight...

Phytophthora nicotianae causes black shank, a serious soil-borne disease, in tobacco. In this study, the Bacillus strain GUMT319 was isolated from rhizosphere of healthy tobacco plants grown field Guizhou with high incidence shank. Genome sequencing revealed that contained single circular chromosome 3,940,023 bp length, 4,053 predicted genes and an average GC content 46.6%. Based on phylogenomic analyses, designated as velezensis . The genome more than 60 13 gene clusters have previously...

Background Agrobacterium tumefaciens T-37 can infect grapes and other fruit trees cause root cancer. Given the pollution damage of chemical agents to environment, use biological control has become an important area focus. Bacillus megaterium L2 is a beneficial biocontrol strain isolated identified in laboratory, which good antibacterial effect on variety plant pathogens. The metabolites were separated purified obtain bioactive compound phenylacetic acid (PAA). Methods potential mechanism PAA...

Soft rot causes significant economic losses in the kiwifruit industry. This study isolated strain CTXW 7-6-2 from healthy tissue; this was a gram-positive bacterium that produced red pigment pulcherrimin. The phylogenetic tree based on 16S ribosomal RNA, gyrA, rpoB, and purH gene sequences identified as of Bacillus subtilis. inhibited hyphal growth pathogenic fungi cause soft rot, namely, Botryosphaeria dothidea, Phomopsis sp., Alternaria alternata, by 81.76, 69.80, 32.03%, respectively....

Rhododendron simsii (indoor azalea) is widely cultivated for its high ornamental value (Xu et al. 2021). In April to May 2023, a leaf spot disease occurred in field study at the Baili Azalea Forest Area (27°12'N, 105°48'E), Guizhou Province, China. About 500 plants were investigated, and results showed that incidence of was 20 ~ 30%. To this disease, 10 showing severe symptoms collected. Initially, round or irregularly shaped brown spots (1 mm). With time, enlarged merged. Symptomatic leaves...

has been widely studied for its potential to protect plants from pathogens. Here, we report the whole genome sequence of

To explore natural product-based pesticide candidates, a series of indole derivatives containing the isoxazoline skeleton at N-1 position were synthesized by 1,3-dipolar [2 + 3] cycloaddition reaction. Their structures characterized melting points (mp), infrared (IR) spectra, proton nuclear magnetic resonance spectra (1H NMR), carbon-13 (13C and high resolution mass spectrometry (HRMS). The single-crystal five compounds presented. Against Tetranychus cinnabarinus Boisduval, compound 3b...

Pepper wilt, caused by Fusarium oxysporum, is an important disease of pepper in many regions the world. A phosphate solubilising Trichoderma strain MHT1134 was selected from 76 isolates rhizosphere as a potential biological control agent against wilt F. oxysporum. The inhibitory rate oxysporum 81.80%. identified hamatum based on morphological features, ITS rDNA and tef-1α sequences. It showed antagonistic effects number fungal pathogens, including Phytophthora capsici, Colletotrichum...

Bacillus velezensis GUAL210 was isolated from the rhizosphere of healthy pepper plants growing in high-incidence anthracnose fields Guizhou, China. could be used as a potential biocontrol agent against and other soil-borne diseases. The genome consisted single circular chromosome 4,011,788 bp length, with an average GC content 46.41%, did not harbor any plasmids. A total 4,115 protein-coding genes, 27 rRNAs, 87 tRNAs, 12 secondary metabolite biosynthesis gene clusters were identified....

Rose black spot is an economically important disease that significantly decreases flower yield. Fungicide and biological control are effective approaches for controlling rose spot. The objective of this study was to evaluate the effect application chemical agents, including Bacillus velezensis (GUAL210), sp. (LKW) fungicide (CP) on rhizosphere fungal community structure edible rose.In study, R. chinensis 'Crimson Glory' taken as research object, field experiment designed by randomized block...

Virus is the most menacing factor for plant, which causes enormous economic losses in agriculture worldwide. Tobacco mosaic virus hazardous among plants that can spread through biological and non-biological sources. TMV ancient huge to pepper cucumber ornamental crops tobacco. It be controlled by reducing population of vector pesticide application. However, rapid usage synthetic chemicals environmental pollution destroys our ecosystem. Consequently, different approaches just like natural...

To explore the use of nonfood plant-derived secondary metabolites for plant protection, a series ester derivatives controlling major migratory agricultural pests were obtained by structural modification andrographolide, labdane diterpenoid isolated from Andrographis paniculata. Compound Id showed good insecticidal activity against fall armyworm Spodoptera frugiperda Smith. Compounds IIa (LC50: 0.382 mg/mL) and IIIc 0.563 mg/mL), acaricidal activities which were, respectively, 13.1 8.9 times...

Abstract BACKGROUND Owing to large amounts of synthetic pesticides being extensively and unreasonably used for crop protection, currently, resistance negative impacts on human health environment safety have appeared. Therefore, development potential pesticide candidates is highly urgent. Herein, a series ester derivatives osthole were designed synthesized as pesticidal agents. RESULTS Six spatial configurations 4′‐( p ‐toluenoyloxy)osthole ( 4b ), m ‐fluorobenzoyloxy)osthole 4f...

Rosa roxburghii Tratt, known as Cili in China, is a fruit crop that grows the mountains of southwest China at altitudes 500 - 2500 m, especially Guizhou province (Huang et al. 2022). In July 2021, leaf spot symptoms were observed on approximately 20 to 30% R. plants field 6,000 m2 Guiding County (107°14'E, 26°45'N), Province, China. Severe can lead excessive drop, significantly weakening tree and adversely affecting its growth quality, which turn result reduced or even lost harvests. The...

ABSTRACT Bacillus cereus 905 is a plant growth-promoting rhizobacterium, isolated from wheat rhizosphere. The draft genome sequence of this strain 5.39 Mb and harbors 5,412 coding sequences.

The poor fidelity of T4 DNA ligase has always limited the simple detection single-nucleotide polymorphisms (SNPs) and is only applicable to some special SNP types. This study developed a highly sensitive specific method for SNPs based on high-fidelity single-stranded circularisation. It used rolling circle amplification (RCA) plus loop-mediated isothermal (LAMP). Surprisingly, cyclisation stage's efficiency greatly improved. ligation was almost perfect via unique pairing pattern between...

Bacillus velezensis strain GUMT319 is a rhizobacteria biocontrol agent that can control tobacco black shank disease. We took as biological fertilizer on Vitis vinifera L. The test group was treated with for one year and the had water treatment. Yields of GUMT319-treated grape groups were significantly increased compared to controls. average length width single fruit, weight 100 fruits, sugar/acid ratio, content vitamin C all in group. pH soil higher contents alkaline hydrolyzable nitrogen...

HomePlant DiseaseVol. 107, No. 8First Report of Gray Spot on Tobacco Caused by Alternaria alstroemeriae in China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst ChinaZhi-Cheng Zhou, Xian-Ying Tang, Shan Hu, Wen-Ya Zhu, Xue-Ping Wu, Wei-Jun Sang, Hai-Xia Ding, and Li-Juan PengZhi-Cheng Zhouhttps://orcid.org/0000-0001-5192-2783Department Plant Pathology, College Agriculture, Guizhou University, Guiyang, 550025, China, Tanghttps://orcid.org/0000-0003-2379-4248Department...

Tea (Camellia sinensis), which originated in southwest of China 60 - 70 million years ago, is widely consumed as a beverage for its potential enhancing effect on human health with rich polyphenol content (Pan et al. 2022). From October to December 2021, disease symptoms similar leaf spot affected the quality and yield tea Puer (102°73 'E, 25°07' N), Yunnan province, China. Based survey, were observed approximately 60% plants 5,700 m2 field. The initially appeared shrinking, yellowing, later...

Chinese rose (Rosa chinensis Jacq.) is cultivated for edible flowers in southwestern China (Zhang et al. 2014). In March 2020, a leaf spot disease was observed on about 3-5% leaves of cultivar 'Mohong' Guizhou Botanical Garden (26°37' 45'' N, 106°43' 10'' E), Guiyang, province, China. The symptomatic plants displayed circular, dark brown lesions with black conidiomata grey centers leaves, and samples were collected. After surface sterilization (0.5 min 75% ethanol 2 3% NaOCl, washed 3 times...