The using of biological preparations based on bacteria the genera Bacillus and Pseudomonas is one most promising directions in fight against pathogens plant diseases. Quite a lot these microorganisms are known, but some cases their application characterized by insufficient activity pathogens, as well reduction number saprophytic soil microbiota, which negatively affects phytosanitary state reduces plants’ productivity. aim work was to study antagonistic individual representatives from...

The marine ecosystem is a promising source for discovering the producers of totally new bioactive compounds. Among significant biodiversity, actinobacteria also are known by huge antagonistic potential. Aim. To study activity isolated from sponges Haliclona spp. against strains indicator prokaryotic and eukaryotic microorganisms. Methods. Antagonistic Staphylococcus aureus АТСС 25923, Micrococcus luteus 4698, Enterococcus faecalis 29212, Bacillus subtilis 6633, Kocuria rhizophila DSM 348,...

The Black Sea is a unique water basin consisting of thin superficial oxygenic layer with moderate salinity, and deep anoxic mass. microbiota the remains relatively understudied, which makes it interesting first all from most practical point view search for producers new biologically active compounds. A strain actinobacteria Streptomyces sp. ONU 561 was isolated surface mussel shells collected in coastal zone Odesa. It demonstrated wide range antagonistic activity, inhibiting growth set...

Today both academic and commercial interests in marine actinobacteria are growing. As they live a unique environment that promotes the synthesis of new biologically active metabolites. The aim work is isolation, primary identification study morphological, cultural, physiological biochemical properties actinobacteria, separate from mussels (Mytilus galloprovincialis) Odessa Bay Black Sea. Methods. Samples collected coastal zone were used as material for isolation actinobacteria. Isolation...

Currently, the search for new alternative effective sources obtaining compounds with antimicrobial activity does not lose its relevance. Aim. To investigate of oils obtained by cold pressing from pumpkin seeds, grape seeds and sea-buckthorn fruits. Methods. Antimicrobial fruits against strains indicator microorganisms Staphylococcus aureus АТСС 25923, Micrococcus luteus 4698, Enterococcus faecalis 29212, Bacillus subtilis 6633, Kocuria rhizophila DSM 3480, Escherichia coli 25922, Proteus...

As a result of military activities, soils are contaminated with substances from explosives and firearm mechanisms, fuel, lubricants, heavy metals, all which have detrimental effects on ecosystems. This article analyzes the main methods bioremediation for such soil. The use soil slurry reactors, land farming technique, composting, biostimulation, bioaugmentation, immobilized microorganisms can eliminate up to 99% toxic through natural or introduced microbiota. duration cleanup process ranges...

The biological method of plant protection is an environmentally safe and priority form in long-term programs to combat pathogens one the important tools for transition organic ecological agriculture Ukraine. Aim. To define antagonistic activity Black Sea actinobacteria against phytopathogenic microorganisms. Methods. 35 strains isolated from different biotopes Odesa Bay collection affected cereal plants microorganisms was determined by block after preliminary cultivation on Gause 2 medium 12...

SummaryThe rapid emergence of resistance by bacterial and fungal pathogens is a serious problem in the health care system, which causes search for new promising producers antimicrobial natural products various ecological niches. Aim. To determine antagonistic activity streptomycetes isolated from biological fouling shell rock mussels Odesa gulf Black Sea. Methods. The 19 14 strains gulf, respectively, were investigated. Streptomycetes pre-cultivated on agar media Gause 1, 2 oat with sea salt...

Aim of this study was to investigate the antagonistic effect bacilli isolated from surface plants and rhizosphere zone soil against pathogens barley wheat diseases. Materials methods. The material for affected samples used isolate phytopathogenic microorganisms, as well healthy bacteria genus Bacillus. Manipulations isolation investigation biological properties both phytopathogens were carried out by traditional microbiological activity strains studied block method. Results. From...

SummaryAim. To investigate the antagonistic properties of multistrain compositions and consortia created on basis probiotic strains bacteria genus Lactobacillus. Methods. A biocompatibility was investigated by co-cultivation lactobacilli MRS-agar medium. create used separately grown daily broth cultures in certain proportions, to - appropriate proportions were cultivated together MRS during 24 h. The activity combinations against 10 test determined well-diffusion method. Results. According...

The review presents data according to the modern sources of literature about bacteria genus Pantoea. general cultural, biochemical and morphological properties this are presented. genome genetic elements, their influence on growth behavior microorganisms considered in detail. It also describes association with Daktulosphaira vitifoliae, a pest grape, examines possible effects grape phylloxera. endophytic epiphytic features presented species illuminated. Pathogenic isolation Pantoea...

Aim. To isolate and investigate the biological properties of Pantoea agglomerans from internal environment grapes tumors selected vineyards Odessa region. Methods. Samples grapevines Arkadia, Moldova souvenirs varieties cut affected veins were presterilized, chopped into fragments, filled a distilled water flask stirred in shaker at 28 °C for 3 hours. It was made 10-times dilutions cultured on surface Petrie`s dishes with nutritients agar. Incubationon °С, 24–48 Conducted quantitative...

Aim. To study the resistance of lactobacilli strains to some aggressive factors gastrointestinal tract in vitro depending on primary isolation source. Methods. 13 isolated from self-fermenting vegetables, meat raw materials, children excrements were used this work. The stability bacteria metabolites digestive system was determined by their cultivation MRS medium with bile, sodium chloride and phenol. Results. vast majority tolerant at 2.5–5.0% NaCl. increasing NaCl concentration 7.5% has...

The aim of this work was determining the degree resistance to antibiotics and heavy metals lactic acid bacteria isolated from Black Sea sponges genus Haliclona sp. Methods. classical microbiological methods were used study cultural biochemical characteristics strains. According total obtained indices, strains assigned Lactobacillus. Identification species carried out by spectrum fatty acids using an automatic microorganism identification system MIDI Sherlock gas chromatography. Based on...

Loss prevention and food quality maintenance are primarily associated with protection against the negativeimpact of microorganisms their metabolites during manufacture storage. In this regard, in recent years, issue thegoods safety is top- priority production. Meat meat products most labour-intensive expensiveto manufacture. Their main components (protein, fat, etc.) a favourable environment for development variety microorganisms.This paper presents results biotechnological property research...

Studies of the activity and interaction marine microorganisms with other organisms provide an understanding functioning biogeochemical processes, food chains symbiosis. Marine microbiome members also have useful properties that can be used to search for produce new products develop processes in biotechnology. Representatives actinobacteria isolated from various sources environment are especially promising this aspect. The aim study was isolate biological overgrowth concrete structures...

Aim of the research was to study antagonistic activity lactobacilli isolated from different sources Southern region Ukraine. Methods. The experiments used 13 strains bacteria genus Lactobacillus sources. determined by well-diffusion method in agar column using test cultures pro- and eukaryotic microorganisms. Acid formation evaluated active titrated acidity when cultured milk. Results. All tested were antagonists against gram-negative Escherichia coli, Pseudomonas aeruginosa, Salmonella...

Aim. Determination of fatty acid composition actinobacteria isolated from the surfaces biological growth Odesa gulf Black Sea, and their identification. Methods. The 31 strains were grown in TSB at 28 ° C 150 rpm for 72 hours. Fatty methyl esters studied determined according to MIS Operating Manual on a gas chromatograph Agilent 7890, identification was performed using system microorganisms MIDI Sherlock. Results. Using chromatographic analysis acids, it found that 27 identified genus...

Aim. To determine the biotechnological potential of lactobacilli isolated from raw meat and collection strains compositions which were created on their bases. Methods. Lactobacilli samples through passages in skim milk sample, followed by MRS -agar broth. The previously precultured -broth with 5% glucose. acid production was determined active titratable acidity, activity coagulation – duration clot formation, antagonistic to opportunistic pathogenic bacteria well-diffusion method,...

Aim. The aim of the work was to study morphological characteristics and cytotoxic activity strain actinobacteria Streptomyces sp. Lim 10 isolated from natural fouling shell rock in Odesa Bay Black Sea. Materials methods. object a marine 10. morphology growth patterns were studied on media Gauze 2, Czapek, Eshbi, TSB ISP (ISP-1–ISP-7). SG medium used accumulate secondary metabolites actinobacteria. Extraction exometabolites culture liquid carried out with ethyl acetate followed by dissolution...

The aim of the work was to determine cytotoxic activity marine actinobacteria exometabolites on human larynx adenocarcinoma Hep-2 and rhabdomyosarcoma RD cell lines. Exometabolites 6 strains Streptomyces sp. were extracted with ethyl acetate their composition determined UltiMate™ 3000 BioRS UPLC System coupled mass spectrometer. Cytotoxic against cancer cells after 24 hours incubation by microscopic examination morphological changes degree monolayer degeneration. from Myt7b strain showed...

Biological features, pathogenesis, methods of detection, identification and control fungi the genus Fusarium, capable causing diseases in various types plants, barley wheat. Information is provided about main mycotoxins synthesized by fusaria, as well ways to reduce their concentration affected grain. The need for development implementation biological controlling fusarium pathogens noted.

Currently, due to climate change and a number of unfavorable environmental conditions, there is tendency decrease the global production wheat - one main grain crops. It undeniable that increase in closely related effectiveness controlling most harmful diseases crops Fusarium wilt, since are no varieties world completely resistance wilt. The development biotechnological approaches obtaining new microbial preparations protect winter from pathogens relevant for biological protection organic...