Abstract Endothelin-1 is a 21-amino acid potent vasoconstrictor peptide produced by vascular endothelial cells. We have cloned the whole length of human preproendothelin-1 (PPET-1) gene and corresponding cDNA determined complete nucleotide sequences. The 2026-nucleotide mRNA for PPET-1 (excluding polY(A) tail) encoded in five exons distributed over 6836 base pairs genome. 5'-flanking region contains (i) octanucleotide sequences phorbol ester-responsive elements, also known as binding...

Gonadotrophin-releasing hormone (GnRH) is considered to have an important role in the control of reproduction salmonid fish, although we do not any direct evidence. To clarify this problem by molecular techniques, first determined nucleotide sequence mRNA encoding precursor salmon-type GnRH (sGnRH) from masu salmon, Oncorhynchus masou. The salmon sGnRH was composed a signal peptide, and GnRH-associated peptide (GAP) which connected Gly-Lys-Arg sequence. amino acid were highly conserved when...

In Drosophila sensory organ development, the balance of activities between proneural genes and repressor defines a cluster as population competent cells for neural development. this study, we report isolation analysis tamou (tam) gene that encodes cell-cell junction-associated protein, which is homologous to mammalian ZO-1, member membrane-associated guanylate kinase homolog family. The tam mutation reduces transcription gene, extramacrochaetae, causes enlargement where supernumerary...

The pathophysiology and symptoms underlying Meniere's disease (MD) manifest as endolymphatic hydrops (EH), potentially impacting acoustic power absorbance in vestibular EH. longitudinal effects of middle ear pressure therapy (MEPT) conservative therapies for EH by magnetic resonance imaging (MRI) on wideband immittance (WAI) were evaluated, their changes compared with clinical symptoms.

The objective of this study was to investigate the relationship between endolymphatic hydrops (EH) and hearing level or vestibular symptoms by focusing on EH herniation into semicircular canal (SCC).Retrospective study.University hospital.The included 1,548 ears 775 patients who underwent magnetic resonance imaging (MRI) examination in our university hospital possible EH. MRI performed 4 hours after intravenous injection a standard dose gadodiamide hydrate and/or 24 intratympanic...

We investigated whether endothelial dysfunction might contribute to the exaggerated vasoconstriction that was induced by administration of norepinephrine at early stage one-kidney, one-clip renal hypertension (1K1C) in rats. also studied role renin-angiotension system this phenomenon. Male Wistar rats were killed 48 hours after induction artery stenosis or sham operation, and ring preparations thoracic aorta obtained. The isometric contraction relaxation aortic strips produced acetylcholine,...

The survey was designed to determine the trend of group B streptococcal (GBS) infection during last 15 years, as well update reality this disease.Questionnaires were administered every 5 years towards neonatal intensive care unit-equipped hospitals. first made from 1983 1987, second one 1988 1992 and third 1993 1997. 15-year questionnaire included a total 831 patients. changes in number patients, ages at onset, diagnosis, serotypes causative agents, presence or absence complicated delivery...

Human tubal epithelial cells in primary culture were transfected with simian virus 40 (SV40) large T antigen plasmid, and an immortalized ciliated cell line, named as NT/T-S, was established without crisis. Transmission electron microscopy proved that NT/T-S had cilia, microvilli, junctional complexes, rough endoplasmic reticula, free ribosomes microtubules. evaluated preliminarily on the basis of co-culture study using surplus embryos at 4- to 8-cell stage our IVF embryo transfer programme....

As a source of hematopoietic stem cells for transplantation, the use peripheral blood (PBSCs) has become routine and comparable to that bone marrow. Recently, elderly patients with hematological malignancies also have been allowed receive minitransplantations nonmyeloablative conditioning regimens under sufficient PBSC infusion. result these minitransplantations, donors chosen increasingly from siblings patients. We analyzed factors influencing condition CD34+ in first days collection 49...

Background: The presence of endolymphatic hydrops (EH) may cause hearing loss and affect the transmission acoustic energy to inner ear.Objectives: Acoustic absorbance on wideband immittance (WAI) was evaluated, focusing especially EH in vestibule.Material methods: A total 32 ears from 16 patients who underwent 3-T magnetic resonance imaging (MRI) evaluate were examined, retrospectively. degree vestibule classified into three grades (no, mild, significant), pure tone audiometry (PTA) WAI...

Background High signals in the inner ear of idiopathic sudden sensorineural hearing loss (ISSNHL) on magnetic resonance imaging (MRI) have been reported, but no quantitative evaluation has yet done.Objectives To evaluate outcomes and cochlear signal intensities 3-T heavily T2-weighted three-dimensional fluid-attenuated inversion recovery (hT2W-3D-FLAIR) patients with ISSNHL.Materials methods Twenty-nine ISSNHL were included. Patients underwent hT2W-3D-FLAIR intravenous gadolinium injection...

Otological diseases including Meniere's disease (MD) involve endolymphatic hydrops (EH), which can be visualized by magnetic resonance imaging (MRI) with gadolinium contrast agents, but the temporal changes of in inner ear have not been evaluated.We investigated permeability blood-perilymph barrier (BPB) ears EH to evaluate severity disturbances.The study included 32 from 16 patients or related who underwent MRI. The BPB was assessed signal-intensity ratio (SIR) at four-time points: before...

We evaluated the inner ear distribution of 17O-labeled saline administered to human tympanic cavity. Magnetic resonance imaging was performed after intratympanic administration in five healthy volunteers and one patient with cochlear endolymphatic hydrops. In all volunteers, water permeated basal turn vestibule at 30 min disappeared gradually within 2-4 h. All participants experienced positional vertigo lasting a few hours days. Visualization space posterior ampulla showed indistinct...

Cells bearing invariant Valpha19-Jalpha33 TCR alpha chains are believed to participate in the regulation of inflammatory autoimmune diseases. In this study, potential produce immunoregulatory cytokines by these cells was characterized order find mechanism underlying their functions. Serum levels IL-4, IL-10, transforming growth factor-beta, IFN-gamma and IL-17 increased mice over-expressing an transgene (Valpha19 Tg) response anti-CD3 antibody injection. NK1.1(+) Valpha19 Tg(+), but not...

BackgroundHigh signal intensity in the endolymphatic duct (ED) is occasionally observed on magnetic resonance imaging (MRI) ears that have otological disorders.ObjectiveThe (SI) ED post-contrast MRI was investigated subjects with various disorders, and meaning of high SI evaluated.Material methods392 patients disorders 21 controls without symptoms underwent 3 T MRI. The SIs cerebellum were measured, ratio (SIR) calculated, SIR ≥4 identified.ResultsA identified 3.7% affected by definite...

Background Listening difficulties (LiD) present in listening and paying attention to spoken information despite normal pure tone audiometry. Endolymphatic hydrops (EH) is a common inner ear condition associated with Ménière’s disease but may also be the asymptomatic ear.Objectives Using magnetic resonance imaging, we investigated EH patients LiD assessed whether severity of was related results auditory processing tests (APTs).Materials Methods 111 no abnormalities on audiometry, displaying...

Objective To summarize the pathophysiological analysis of idiopathic sudden sensorineural hearing loss (ISSNHL) by magnetic resonance imaging (MRI), focusing on findings high signal or endolymphatic hydrops (EH) in inner ear. Methods We published studies our research group regarding ISSNHL MRI and review related clinical articles that have reported significantly existence EH ears with ISSNHL. Results Pre-contrast may indicate minor hemorrhage increased permeability surrounding vessels to...

The potential mechanism of augmented response on cervical vestibular evoked myogenic (cVEMP) testing and its decrease following treatment in a patient with Meniere's disease (MD) are discussed. Changes static posturography cVEMP before after glycerol drip 69-year-old man unilateral MD, which significant endolymphatic hydrops (EH) was confirmed magnetic resonance imaging (MRI) the diseased side, were evaluated. Values total locus lengths, areas postural sway, their Romberg ratios decreased...

Clinical findings on cartilage conduction hearing aids (CCHAs) have gradually become clear; however, few reports include a large number of cases. This study included 91 ears from 69 patients who underwent CCHA fitting in our hospital. Their were divided into six groups (i.e., bilateral aural atresia or severe canal stenosis, unilateral chronic otitis media externa with otorrhea, sensorineural loss, mixed and conductive loss) according to their clinical diagnosis type loss. Most diagnoses...