A lignocellulose liquid hot water (LHW) pretreatment process was strengthened by the direct recycling of spent liquor. The use rich liquor and its byproduct acetic acid proposed as a method strengthening LHW pretreatments, which can reduce energy consumption while producing less wastewater. results showed that glucose yield increased from 80.82% to 85.44% during enzymatic hydrolysis after had been recycled three times, at point content 8.1 g/L. When reused furfural 5-hydroxymethyl (HMF)...

Bacteriophages are the most abundant entities in biosphere, and many genomes of rare novel bacteriophages have been sequenced to date. However, bacteriophage functional genomics has limited by a lack effective research methods. Clustered regularly interspaced short palindromic repeat/CRISPR-associated gene (CRISPR-Cas) systems provide with new mechanism for attacking host bacteria as well tools study genomics. It reported that not only driving elements evolution prokaryote CRISPR arrays but...

Abstract Ecto-5′-nucleotidase (NT5E) is a glycosylphosphatidylinositol anchored cell surface protein, and has been suggested to be dysregulated in most types of human cancer including gastric cancer. The aim the present study was more evidence about clinical prognostic value patients, preliminarily explore biological function cells. In our study, high expression observed tissues lines, respectively, compared with normal mucosa Meanwhile, TCGA database also indicated that levels were notably...

Gastric adenocarcinoma is an important death-related cancer. To find factors related to survival and prognosis, thus improve recovery prospects, a powerful signature needed. DNA methylation plays role in gastric processes development, here we report on the search for significant gene aid with earlier diagnosis of patients. A Cox proportional risk regression analysis random forest algorithm were used analyze patients' data from The Cancer Genome Atlas, public database. consisting five genes...

Abstract Background It is increasingly evidenced that long non-coding RNAs (lncRNAs) play an important role in various diseases. LncRNA LINC01194 acts as oncogene several cancer types. Nevertheless, the of lung adenocarcinoma (LUAD) has not yet been revealed. Methods qRT-PCR was used to detect expression LINC01194, miR-641 and SETD7 mRNA, while western blot exploited examine protein level. Cell proliferation detected by colony formation EdU assays. Transwell assays cell migration invasion....

To improve the utilization of phragmites in biorefineries, strengthening effect byproduct-organic acids on subcritical liquid hot water (SLHW) pretreatment was explored. The were accumulated during direct recycling pretreated liquid, which could reduce consumption. acids, including acetic acid and lactic acid, used to strengthen SLHW raw at different temperatures (160–200 °C) times (20–90 min) process. During pretreatment, high yield C-5 sugars 60.66% severity 4.85 (200 °C, 75 min). However,...

Parkinson's disease (PD) is a that involves brain damage and associated with neuroinflammation, mitochondrial damage, cell aging. However, the pathogenic mechanism of PD still unknown. Sequencing data proteomic can describe fluctuation molecular abundance in diseases at mRNA level protein level, respectively. In order to explore new targets pathogenesis PD, study analyzed changes from database by combining transcriptomic analysis. Differentially expressed genes differentially abundant...

Identifying cancer-related miRNAs (or microRNAs) that precisely target mRNAs is important for diagnosis and treatment of cancer. Creating novel methods to identify candidate becomes an imminent Frontier researches in the field. One major obstacle lies integration state-of-the-art databases. Here, we introduce a method, MIMRDA, which incorporates miRNA mRNA expression profiles predicting miRNA-disease associations key miRNAs. As proof-of-principle study, use MIMRDA method analyze TCGA...

Colon ascendens stent peritonitis (CASP) surgery induces a leakage of intestinal contents which may cause polymicrobial sepsis related to post-operative failure remote multi-organs (including kidney, liver, lung and heart) possible death from systemic syndromes. Mechanisms underlying such phenomena remain unclear. This article aims elucidate the mechanisms CASP-model by analyzing real-world GEO data (GSE24327_A, B C) generated mice spleen 12 hours after CASP-surgery in septic MyD88-deficient...

Background No attention has been paid on comparing a set of genome sequences crossing genetic components and biological categories with far divergence over large size range. We define it as the systematic comparative genomics aim to develop methodology. Results First, we create method, GenomeFingerprinter, unambiguously produce three-dimensional coordinates from sequence, followed by one plot six two-dimensional trajectory projections, illustrate fingerprint given sequence. Second, concepts...

Assessing correctness of an assembled chromosome architecture is a central challenge. We create geometric analysis method (called GenomeLandscaper) to conduct landscape genome-fingerprints maps (GFM), trace large-scale repetitive regions, and assess their impacts on the global architectures chromosomes. develop alignment-free for phylogenetics analysis. The human Y chromosomes (GRCh.chrY, HuRef.chrY YH.chrY) are analysed as proof-of-concept study. construct galaxy (GGFM) them, compatibility...

Systems characterization of immune landscapes in health, disease and clinical intervention cases is a priority modern medicine. High-throughput transcriptomes accumulated from gene-knockout (KO) experiments are crucial for deciphering target KO signaling pathways that impaired by genes at the systems-level. There demand integrative platforms. This article describes PathwayKO platform, which has integrated state-of-the-art methods pathway enrichment analysis, statistics visualizing analysis...

e15546 Background: Ovarian cancer typically has an excellent response to chemotherapy but a high rate of recurrence with patients ultimately dying from chemoresistance. The objective this study was investigate the relationship between histone modification, DNA methylation and damage repair that occur in platinum resistant ovarian cell lines treated epigenetic modifiers combination low dose cisplatin develop new approaches for cancer. Methods: We selected two common Trichostain A (TSA),...