A5052764415- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Food Allergy and Anaphylaxis Research
- Dermatology and Skin Diseases
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Transgenic Plants and Applications
- T-cell and B-cell Immunology
- Mast cells and histamine
- vaccines and immunoinformatics approaches
- Contact Dermatitis and Allergies
- Influenza Virus Research Studies
- IL-33, ST2, and ILC Pathways
- Cytokine Signaling Pathways and Interactions
- Nail Diseases and Treatments
- Phagocytosis and Immune Regulation
- Cell death mechanisms and regulation
- Biochemical effects in animals
- Probiotics and Fermented Foods
- Lung Cancer Research Studies
- Toxin Mechanisms and Immunotoxins
- RNA Research and Splicing
- Mycotoxins in Agriculture and Food
- Mycobacterium research and diagnosis
- Genetically Modified Organisms Research
State Key Laboratory of Respiratory Disease
2013-2024
Second Affiliated Hospital of Guangzhou Medical University
2013-2024
Guangzhou Medical University
2013-2024
Guangzhou Experimental Station
2016
Shanghai Jiao Tong University
2012
Cell-in-cell structures are created by one living cell entering another homotypic or heterotypic cell, which usually leads to the death of internalized specifically through caspase-dependent (emperitosis) lysosome-dependent (entosis). Although entosis has attracted great attention, its occurrence is controversial, because line used in study (MCF-7) deficient caspase-3. We investigated this issue using MCF-7 and A431 lines, often display cell-in-cell invasion, have different levels caspase-3...
A lower adherence rate existed in patients receiving allergen-specific immunotherapy due to its lengthy period and adverse effects even though it is the only curative treatment for IgE-mediated allergies. Therefore, exploring innovative routes necessary.
Abstract Multiple studies have addressed the vital role of Nod-like receptor protein 3(NLRP3)/caspase-1/IL-1β signaling in asthma. Yet, NLRP3/caspase-1 toluene diisocyanate (TDI)-induced asthma is still obscure. The aim this study to investigate axis TDI-induced Using an established murine model as described previously, we gave asthmatic mice a highly selective NLRP3 inhibitor, MCC950, well specific caspase-1 inhibitors VX-765 and Ac-YVAD-CHO for therapeutic purposes. Airway resistance was...
Abstract Asthma is a common allergic disease characterized by airway hypersensitivity and remodeling. Ferroptosis regulated death marked iron accumulation lipid peroxidation. Several environmental pollutants allergens have been shown to cause ferroptosis in epithelial cells, but the relationship between birch pollinosis asthma poorly defined. Here, for first time, we identified of type II alveolar cells mice with Bet v 1-induced asthma. Further analysis revealed that treatment ferrostatin-1...
Abstract Summary: SORTALLER is an online allergen classifier based on family featured peptide (AFFP) dataset and normalized BLAST E-values, which establish the vectors for support vector machine (SVM). AFFPs are allergen-specific peptides panned from irredundant allergens harbor perfect information with noise fragments eliminated because of their similarity to non-allergens. performed significantly better than other existing software reached a balance high specificity (98.4%) sensitivity...
The association of colitis with colorectal cancer has become increasingly clear mast cells being identified as important inflammatory in the process. In view relationship between and cancer, we studied effect mechanisms development colon cancer. Functional mechanistic insights were gained from ex vivo studies cell interactions CT26 cells. Further evidence was reversely obtained targeted Fcε-PE40 chimeric toxin. Experiments revealed could induce tumor proliferation invasion. Cancer...
Accumulating evidence has suggested that toll-like receptor 4 (TLR4) is critically involved in the pathogenesis of asthma. The aim this study was to investigate role TLR4 toluene diisocyanate (TDI)-induced allergic airway inflammation.TLR4-/- and wild-type (WT) C57BL/10J mice were sensitized challenged with TDI generate a TDI-induced asthma model. B-cell lymphoma 2 (Bcl-2) inhibitors, ABT-199 (4 mg/kg) ABT-737 mg/kg), intranasally given TDI-exposed TLR4-/- after each challenge.TDI exposure...
Vast amounts of allergen sequence data have been accumulated, thus complicating the identification specific allergenic proteins when performing diagnostic allergy tests and immunotherapy. This study aims to rank importance/potency allergens so as logically reduce number and/or sources. Meta-analysis 62 sources used for intradermal testing on 3,335 allergic patients demonstrated that in southern China, mite, sesame, spiny amaranth, Pseudomonas aeruginosa , house dust account 88.0% 100%...
Aim . To investigate differences in the efficacy of sublingual immunotherapy with Dermatophagoides farinae drops monosensitized and polysensitized allergic rhinitis patients. Methods The patients enrolled study were treated for more than one year by (SLIT) using divided into a group (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn>20</mml:mn></mml:math>) id="M2"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn>30</mml:mn></mml:math>)....
Staphylococcal enterotoxin A is well known as a superantigen and able to be used for cancer immunotherapy. In this study, recombinant was genetically conjugated epidermal growth factor produce chimeric protein A–epidermal expressed in Escherichia coli. The purified using Strep-Tactin affinity chromatography Endotoxin Removal Resin identified by sodium dodecyl sulfate-polyacrylamide gel electropheresis liquid chromatography-tandem mass spectrometry analysis. Furthermore, vitro experiments...
Peanut ( Arachis hypogaea ) is one of the most common food allergens that can induce fatal anaphylaxis, and Ara h 2 major allergen components involved in peanut allergy. The aim this study was to develop a quantitative method for detecting using monoclonal antibodies against 2. splenocytes immunized mice were fused with myeloma cells (SP2/0), stable mAb-producing clones obtained by limiting dilution. mAbs isolated from mouse ascites, specificity confirmed immunoblotting. Five high purity...
Monoclonal antibodies (mAbs) are needed for the quantitation of environmental allergens precise diagnosis and immunotherapy. In this study, we produced purified monoclonal against Der f 2, one major house dust mite Dermatophagoides farina, in order to develop an assay detection allergen. BALB/c mice were immunized four times with protein 2 together adjuvant after which splenocytes collected fused SP2/0 (myeloma cells) presence polyethylene glycol (PEG). The cells selected...
Staphylococcal enterotoxin A (SEA) is a well-known superantigen and stimulates human peripheral blood mononuclear cells (PBMCs) involving in the pathogenesis of inflammatory disorders cancer.To better understand biological activities SEA possible intracellular mechanisms by which plays its roles conditions like staphylococcal and/or autoimmune immunotherapy.Recombinant (rSEA) was expressed prokaryotic expression system effects on cytokine chemokine production examined Enzyme-linked...