A5031211693- Dermatology and Skin Diseases
- Allergic Rhinitis and Sensitization
- T-cell and B-cell Immunology
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Autoimmune and Inflammatory Disorders Research
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Urticaria and Related Conditions
- Hematopoietic Stem Cell Transplantation
- CAR-T cell therapy research
- Respiratory viral infections research
- Sepsis Diagnosis and Treatment
- Neonatal and Maternal Infections
- Ocular Diseases and Behçet’s Syndrome
- Long-Term Effects of COVID-19
- Immune cells in cancer
- Contact Dermatitis and Allergies
- Inflammatory Myopathies and Dermatomyositis
- Immunodeficiency and Autoimmune Disorders
- Mass Spectrometry Techniques and Applications
- interferon and immune responses
- Retinal and Optic Conditions
- Advanced Biosensing Techniques and Applications
University Medical Center Utrecht
2015-2025
Wilhelmina Children's Hospital
2011-2025
Utrecht University
2021-2024
Center for Translational Molecular Medicine
2020-2023
University Hospital Heidelberg
2018-2020
Heidelberg University
2018-2020
Boston Children's Hospital
2010
Boston University
2010
Leiden University Medical Center
2007-2008
The generation of naive T cells is dependent on thymic output, but in adults, the cell pool primarily maintained by peripheral proliferation. Naive have long been regarded as relatively quiescent cells; however, it was recently shown that IL-8 production a signatory effector function cells, at least newborns. How this functional signature relates to dynamics and aging unknown. Using cohort children adolescents who underwent neonatal thymectomy, we demonstrate CD4+ compartment healthy humans...
BackgroundAtopic dermatitis (AD) is a highly heterogeneous disease, both clinically and biologically, whereas patients are still being treated according to "one-size-fits-all" approach. Stratification of into biomarker-based endotypes important for future development personalized therapies.ObjectiveOur aim was confirm previously defined serum patient clusters in new cohort with AD.MethodsA panel 143 biomarkers measured by using Luminex technology samples from 146 severe AD (median Eczema...
Dupilumab, a mAb targeting IL-4 receptor alpha (IL-4Rα), markedly improves disease severity in patients with atopic dermatitis. However, the effect of IL-4Rα blockade on dynamics circulating skin-homing T cells, which are crucial players pathologic mechanism dermatitis, has not been studied yet. In addition, it remains unknown whether dupilumab treatment induces long-lasting T- and B-cell polarization. Therefore, we short- long-term effects expression T-cell cytokine production within total...
There is an urgent medical need to differentiate active tuberculosis (ATB) from latent infection (LTBI) and prevent undertreatment overtreatment. The aim of this study was identify biomarker profiles that may support the differentiation between ATB LTBI validate these signatures.The discovery cohort included adult individuals classified in four groups: (n = 20), without prophylaxis (untreated LTBI; n after completion (treated healthy controls (HC; 20). Their sera were analyzed for 40...
Atopic dermatitis (AD) is a chronic inflammatory skin disease for which new targeted therapies are currently available. Due to the increased rates of ocular surface (OSD) reported during treatment with these treatments, more insight into occurrence and pathomechanism OSD in moderate-to-severe AD patients needed. Therefore, this review's first part highlights that most already have characteristics before starting treatment. Remarkably, not all report symptoms. associated less conjunctival...
The proximity extension assay (PEA) enables large-scale proteomic investigations across numerous proteins and samples. However, discrepancies between measurements, known as batch-effects, potentially skew downstream statistical analyses increase the risks of false discoveries. While implementing bridging controls (BCs) on each plate has been proposed to mitigate these effects, a clear method for utilizing this strategy remains elusive. Here, we characterized batch effects in PEA proteomics...
Abstract Background Autologous hematopoietic stem cell transplantation (aHSCT) has emerged as an effective therapeutic strategy for patients with severe chronic inflammatory conditions that are refractory to conventional treatment, including Crohn’s disease. The success of aHSCT is thought be grounded in resetting the break immunological tolerance, but exact mechanisms underlying a favourable response remain incompletely understood. Methods We followed immune reconstitution 9 disease from...
<title>Abstract</title> Intramuscular monoclonal antibodies (mAbs) can prevent respiratory infections but are costly and not globally available. The development of intranasal mAbs offers a promising cost-reducing non-invasive alternative to systemic administration, though clinical evidence is lacking. Intranasal palivizumab prevented syncytial virus (RSV) infection in mice healthy infants. This proof-of-concept study evaluates efficacy RSV infection. In this randomized, double-blind,...
Global health clinical research is commonly led by high-income countries (HICs) as low- and middle-income (LMICs) face barriers to participate, including lack of financial human capacity environment. Respiratory syncytial virus (RSV) vaccine development also HICs, while LMICs carry the burden life-threatening disease. Representative trials strengthening in are needed ensure global access equity. This study aims transfer an RSV neutralisation assay, which uses live cells with inherent high...
Controlled human infection models (CHIMs) are an important tool for accelerating clinical development of vaccines. CHIM costs driven by quarantine facilities but may be reduced performing in the outpatient setting. Furthermore, CHIMs offer benefits beyond costs, such as a participant-friendly approach and increased real-world aspect. We analyze safety, logistic ethical risks respiratory syncytial virus (RSV) A review literature identified involving pathogens. RSV transmission risk was...
// Joshua N. Kellner 1 , Eveline M. Delemarre 2 Eric Yvon 3 Stefan Nierkens Jaap J. Boelens 4 Ian McNiece Amanda Olson Yago Nieto Ciurea Uday Popat Sairah Ahmed Richard Champlin Jennifer Ramos Mitsutaka Nishimoto 5 Hongbing Ma Zeng Ke Peter Thall 6 Joseph D. Khoury 7 Robert Negrin 8 Borje Andersson and Simrit Parmar 1, Department of Experimental Therapeutics, University Texas MD Anderson Cancer Center, Houston, TX, USA Laboratory Translational Immunology, Medical Center Utrecht,...
The patho-mechanism of ocular surface disease (OSD) in dupilumab-treated atopic dermatitis (AD) patients remains unclear. aim this study is to measure dupilumab levels tear fluid and serum, relate these findings the severity OSD during treatment AD patients.
Peptide-based immune tolerance induction is considered an attractive treatment option for autoimmune diseases. The authors have developed a novel method that can enhance the of protective peptide-specific T-cell responses, using rat arthritis model. focused on Toll-like receptor 9 ligand CpG, which was shown to stimulate regulatory proliferation when added plasmacytoid dendritic cells (pDC) in-vitro cultures.
During the past decade, new more targeted therapies for treatment of atopic dermatitis (AD) have been developed and are currently under investigation in clinical trials. However, comparison results from different studies remains challenging, given substantial variation clinician-rated outcome measures that used, high inter- intra-observer dissimilarities.1 An objective consistent measurement is hence needed to successfully compare options. Serum thymus activation-regulated chemokine...
Abstract Coronavirus disease 2019 (COVID-19) is a rapidly emerging pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Critical COVID-19 thought to be associated with hyper-inflammatory process that can develop into distress syndrome, critical normally mediated dysfunctional neutrophils. This study tested hypothesis whether neutrophil compartment displays characteristics of hyperinflammation in patients. Therefore, prospective was performed on all patients...
Despite intensive treatment, including consolidation immunotherapy (IT), prognosis of high-risk neuroblastoma (HR-NBL) is poor. Immune status patients over the course and thus immunological features potentially explaining therapy efficacy, are largely unknown. In this study, dynamics immune cell subsets their function were explored in 25 HR-NBL at diagnosis, during induction chemotherapy, before high-dose IT. The cells varied between patients. IL-2- GM-CSF-containing IT cycles resulted...
Abstract Signal inhibitory receptor on leukocytes-1 (SIRL-1) is an immune expressed human granulocytes and monocytes that dampens antimicrobial functions. We previously showed sputum neutrophils from infants with severe respiratory syncytial virus (RSV) bronchiolitis have decreased SIRL-1 surface expression compared blood rapidly lost in vitro activated neutrophils. This led us to hypothesize lose by ectodomain shedding. Here, we developed ELISA measured the concentration of soluble...
Severe uveitis is a rare complication of interleukin-4 receptor alpha blocking by dupilumab in topic dermatitis (AD) patients. The aim this study was to describe five moderate-to-severe AD patients who developed during treatment and compare the proteomic profile aqueous humor (AqH) dupilumab-associated (n=3/5 available samples) with non-infectious (n=27) cataract controls (n=11). Included were treated at University Medical Center Utrecht (the Netherlands). Active complicated serous...
Abstract Introduction Bacterial infections are frequently seen in the emergency department (ED), but can be difficult to distinguish from viral and some non-infectious diseases. Common biomarkers such as c-reactive protein (CRP) white blood cell (WBC) counts fail aid differential diagnosis. Neutrophil CD64 (nCD64), an IgG receptor, is suggested more specific for bacterial infections. This study investigated if nCD64 other infectious diseases ED. Methods All COVID-19 suspected patients who...