A5017038598- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Liver physiology and pathology
- Mesenchymal stem cell research
- Bone Tissue Engineering Materials
- Muscle Physiology and Disorders
- Angiogenesis and VEGF in Cancer
- Neurobiology and Insect Physiology Research
- Ion channel regulation and function
- Cell Adhesion Molecules Research
- Genomics and Chromatin Dynamics
- Organ Transplantation Techniques and Outcomes
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Osteoarthritis Treatment and Mechanisms
- Periodontal Regeneration and Treatments
- Biotin and Related Studies
- Pluripotent Stem Cells Research
- RNA modifications and cancer
- Signaling Pathways in Disease
- Pancreatic function and diabetes
- Silk-based biomaterials and applications
- Glycosylation and Glycoproteins Research
- Nasal Surgery and Airway Studies
Massachusetts General Hospital
2011-2023
Harvard University
2010-2023
Lemuel Shattuck Hospital
2017
Boston Children's Hospital
2016
Pfizer (United States)
2016
Syracuse University
2016
Draper Laboratory
2010-2016
Amgen (United States)
2016
Zero to Three
2015
Harvard University Press
2003
Clinical protocols utilize bone marrow to seed synthetic and decellularized allogeneic grafts for enhancement of scaffold remodeling fusion. Marrow-derived cytokines induce host neovascularization at the graft surface, but hypoxic conditions cause cell death core. Addition cellular components that generate an extensive primitive plexus-like vascular network would perfuse entire upon anastomosis could potentially yield significantly higher-quality grafts. We used a mouse model develop...
Abstract Poly(glycerol sebacate) (PGS), a promising scaffold material for soft tissue engineering applications, is soft, tough elastomer with excellent biocompatibility. However, the rapid in vivo degradation rate of PGS limits its use as material. To determine impact crosslink density on rate, family materials was synthesized by incrementally increasing curing time from 42 to 144 h, at 120°C and 10 mTorr vacuum. As expected, became stiffer, tougher, stronger time. disks were subcutaneously...
The initiation of mammalian puberty requires the activation hypothalamic neurons secreting neuropeptide luteinizing hormone-releasing hormone (LHRH). It is thought that this caused by changes in trans-synaptic input to LHRH neurons. More recently, it has been postulated pubertal increase secretion female animals also neuron-glia signaling mediated growth factors epidermal factor (EGF) family and their astrocytic erbB receptors. Although appears clear functional erbB1 receptors are necessary...
To study the specification of inflow structures in heart we generated transgenic animals harboring human alkaline phosphatase (HAP) gene driven by proximal 840 bp a quail SMyHC3 promoter. In mice, SMyHC3-HAP reporter was expressed posterior precursors at 8.25 dpc, sinus venosa and atrium 8.5 9.0 atria from 10.5 dpc onwards. transgene expression overlapped synthesis endogenous response to retinoic acid (RA) heart, as determined antibodies directed against key RA synthetic enzyme staining...
Human lung tumors PR310 and PR371 maintained in nude mice contain activated c-K-ras oncogenes detectable by the ability of their DNAs to induce morphological transformation NIH 3T3 mouse fibroblasts. Using phage libraries constructed with DNA from fibroblast transformants, we have isolated human sequences that span greater than 40 kilobase pairs oncogene. Based on conservation these conclude transforming oncogene resides within a 43- 46-kilobase-pair region. No clear differences were...
Approximately 10% of all bacterial genomes sequenced thus far contain a secondary replicon. This property populations vastly increases genomic diversity within phylogenetically narrow groups. Members the genus Variovorax have extensive heterogeneity in genome architecture, including isolates containing plasmids, megaplasmids, and chromids. Many NCBI database were generated using only short-read data assembled to permanent draft stage. We acquired set these used MinION long-read sequencing...
Advancement of engineered ear in clinical practice is limited by several challenges. The complex, largely unsupported, three-dimensional auricular neocartilage structure difficult to maintain. Neocartilage formation challenging an immunocompetent host due active inflammatory and immunological responses. large number autologous chondrogenic cells required for engineering adult human-sized presents additional challenge because primary chondrocytes rapidly dedifferentiate during vitro culture....
Availability of grafts and morbidity at the donor site limit autologous transplantation in patients requiring bone reconstruction. A tissue-engineering approach can overcome these limitations by producing bone-like tissue custom shape size from isolated cells. Several hydrogels facilitate osteogenesis on porous scaffolds; however, relative suitability various has not been rigorously assessed. Fibrin glue, alginate, collagen I were mixed with swine marrow–derived differentiated mesenchymal...
Decellularized extracellular matrix (ECM) biomaterials are increasingly used in regenerative medicine for abdominal tissue repair. Emerging ECM with greater compliance target surgical procedures like breast and craniofacial reconstruction to enhance aesthetic outcome. Clinical studies report improved outcomes newly designed scaffolds, but their comparative biological characteristics have received less attention. In this study, we investigated scaffolds derived from dermis (AlloDerm...
Small facial skeletal muscles often have no autologous donor source to effect surgical reconstruction. Autologously derived could be engineered for replacement tissue, but must vascularized and innervated functional. As a critical step, muscle mimic the morphology, protein gene expression, function of native muscle. This study utilized self-assembly process engineer three-dimensional (3D) from statically strained cell monolayer. Primary mouse myoblasts (PMMs) embryonic fibroblasts (MEFs)...
In Brief Objective: This study evaluates a novel liver-assist device platform with microfluidics-modeled vascular network in femoral arteriovenous shunt rats. Summary of Background Data: Liver-assist devices clinical trials that use pumps to force separated plasma through packed beds parenchymal cells exhibited significant necrosis negative impact on function. Methods: Microelectromechanical systems technology was used design and fabricate supports hepatic compartment nanoporous membrane....
Our goal was to engineer cartilage in vivo using auricular chondrocytes that underwent clinically relevant expansion and methodologies could be easily translated into health care practice.Sheep human were isolated from biopsies expanded vitro. To reverse dedifferentiation, cells either mixed with cryopreserved P0 at the time of seeding onto porous collagen scaffolds or proliferated basic fibroblast growth factor (bFGF). After 2-week vitro incubation, seeded implanted subcutaneously nude mice...
Adequate biomaterials for tissue engineering bone and replacement of in clinical settings are still being developed. Previously, the combination mesenchymal stem cells hydrogels calcium-based both vitro vivo experiments has shown promising results. However, results may be optimized by careful selection material combination.β-Tricalcium phosphate scaffolds were three-dimensionally printed with five different hydrogels: collagen I, gelatin, fibrin glue, alginate, Pluronic F-127. The had eight...
Myogenic factor genes were found to respond differentially electrical stimulation of denervated chick skeletal muscle. Myogenin gene activity declined rapidly ( t ½:~2 min), comparable the rate acetylcholine receptor (AChR) inactivation, while other myogenic bHLH either lost more slowly (MyoD) or not at all (myf5, herculin). Protein kinase C (PKC) is known couple membrane AChR inactivation; myogenin transcription was also blocked by PKC activator PMA, whereas electrostimulation remained...
Abstract Biological surgical scaffolds are used in plastic and reconstructive surgery to support structural reinforcement regeneration of soft tissue defects. Macrophage fibroblast cell populations heavily regulate scaffold integration into host following implantation. In the present study, biological response a commercially available (Meso BioMatrix Surgical Mesh (MBM)) was investigated for up 9 weeks after subcutaneous implantation; this promoted superior migration infiltration previously...