A5036352235- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Antimicrobial Peptides and Activities
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- SARS-CoV-2 detection and testing
- Probiotics and Fermented Foods
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Toxin Mechanisms and Immunotoxins
- MicroRNA in disease regulation
- Plant Virus Research Studies
- Salmonella and Campylobacter epidemiology
- Invertebrate Immune Response Mechanisms
- Gut microbiota and health
- Immune Response and Inflammation
- Clostridium difficile and Clostridium perfringens research
- T-cell and B-cell Immunology
- Retinal and Optic Conditions
- RNA Interference and Gene Delivery
- Aquaculture disease management and microbiota
- Viral Infectious Diseases and Gene Expression in Insects
- Immune responses and vaccinations
Centro de Investigación en Alimentación y Desarrollo
2013-2025
ORCID
2022
Porcine circovirus type 2 (PCV2) is a ubiquitous pathogen, and co-infections with the emerging PCV3 are increasingly reported. Both PCV2 have been implicated in reproductive failure, yet diagnostic criteria for remain under development. While fetal or neonatal antibody detection recognized indicator of transplacental infection multiple species, appears to be an exception due possible transfer maternal antibodies. This study evaluated IgG, IgA, IgM antibodies heart, kidney, lung, spleen...
Chagas disease is a neglected caused by the parasite Trypanosoma cruzi, public health problem in both endemic and non-endemic countries. In Mexico, southern region considered endemic, cases are frequently reported; however, northwestern region, only few confirmed annually. This study describes, for first time, Discrete Typing Unit (DTU) of cruzi volunteer blood donor rejected being reactive Mexico. Seroreactivity was using “in-house” ELISAs which employed three different antigens: total...
Abstract Food‐borne outbreaks caused by virus have been associated with the consumption of fresh produce. However, it is difficult to track source contamination due lack sensitive methods detection. In this study, we evaluated presence norovirus ( NoV ), rotavirus RV ) and hepatitis A HAV in produce from northwestern part M exico reverse transcription quantitative PCR RT ‐ qPCR ). Fresh was sampled field packinghouse facilities during different stages packing process. Surfaces equipment, as...
The SARS-CoV-2 virus was detected for the first time in December 2019 Wuhan, China. Currently, this has spread around world, and new variants have emerged. This pandemic provoked rapid development of diagnostic tools, therapies vaccines to control disease called COVID-19. Antibody detection by ELISA been broadly used recognize number persons infected with or evaluate response vaccinated individuals. As spread, questions arose, such as prevalence antibodies after natural infection induced...
This study reports the isolation and characterization of a human monoclonal antibody (mAb) called 19n01. mAb was isolated by using single-cell RNAseq B cells from donors infected with ancestral strain. possesses potent broad capacity to bind neutralize all previously circulating variants concern (VOCs), including Omicron sublineages BA.1, BA.2, BA.4/5. The pseudovirus neutralization assay revealed robust against G614 strain, BA.4/5, inhibitory concentration (IC50) values ranging 0.0035...
Objectives: This study investigated the dynamics of porcine circovirus type 2 (PCV2) and PCV3 on a commercial farm following PCV2 vaccination. Methods: Serum samples from 35 pigs, starting at 3 weeks age, were collected weekly until 21 age. Oral fluids six pens pigs same age also analyzed. Viral DNA was assessed in pooled sera individual oral fluid samples, while antibodies (IgG IgA) measured serum fluids. Productive parameters, including mortality cumulative mortality, evaluated. Results:...
Activation of the immune system using antigen targeting to dendritic cell receptor DEC205 presents great potential in field vaccination. The objective this work was evaluate immunogenicity and protectiveness a recombinant mouse x pig chimeric antibody fused with peptides structural nonstructural proteins porcine respiratory reproductive syndrome virus (PRRSV) directed DEC205+ cells. Priming booster immunizations were performed three weeks apart administered intradermally neck area. All pigs...
The purpose of this study was to evaluate the extent cytokine induction and T cell proliferation in lactic acid bacteria (LAB)-stimulated porcine peripheral blood mononuclear cells (PBMCs). Porcine PBMCs were stimulated with phytohaemagglutinin (PHA) then incubated Bifidobacterium angulatum, Enterococcus faecalis Lactobacillus reuteri. A decrease mRNA expression interferon-gamma (IFN-γ) detected; however, only a significant increase (P<0.05) interleukin (IL)-10 found E. faecalis. No changes...
The purpose of this study was to evaluate the humoral and cellular responses commercial multiparous hyper-immunized sows against peptides from non-structural (nsp) structural proteins porcine reproductive respiratory syndrome virus (PRRSV). We selected with different numbers parities a farm. Management practices on farm include use MLV vaccine four times per year, plus two vaccinations during acclimation period. response evaluated via antibody recognition nsp proteins, assessed by measuring...
Dendritic cell (DC) targeting by DEC205+ cells effectively promotes the internalization of antigens that may trigger a specific immune response. In this study, we evaluated ability recombinant antibody, anti-DEC205 (rAb ZH9F7), to cellular endocytosis in subpopulations DCs and targeted after intradermal injection subsequent migration toward lymph nodes. Furthermore, response was pigs application antigenized rAb ZH9F7 combined with porcine circovirus type 2 cap antigen ZH9F7-Cap). We...
Here, we performed single-cell RNA sequencing of S1 and receptor binding domain protein-specific B cells from convalescent COVID-19 patients with different clinical manifestations. This study aimed to evaluate the role developmental pathway atypical memory (MBCs) in response severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The results revealed a proinflammatory signature across cell subsets associated disease severity, as evidenced by upregulation genes such GADD45B,...
Porcine circovirus type 3 (PCV3) is a nonenveloped virus of the Circoviridae family. This has been identified in pigs different ages and with several clinical manifestations disease or even apparently healthy pigs. While PCV3 was first reported 2015, retrospective studies have before that year. The earliest report indicates circulated swine farms since 1996. In this study, we evaluated presence samples collected Mexico 2008, 2020, 2021. study assessed DNA by qPCR antibodies against CAP...
SARS-CoV-2 infects humans and a broad spectrum of animal species, such as pets, zoo animals, nondomestic animals. Monitoring infection in animals is important terms the risk interspecies transmission emergence new viral variants. Economical, fast, efficient, sensitive diagnostic tests are needed to analyze infection. Double-antigen sandwich ELISA has advantage being multispecies can be used for detecting infections caused by pathogens that infect several hosts. This study aimed develop...
Objective In this study, we investigated the impact of SARS-CoV-2 vaccination on seroprevalence in a cohort healthcare workers (HCW) at an ophthalmic medical center.Methods IgG antibodies against N, S1, and S2 antigens as well their serum neutralizing activity were determined.Results present observed that 98.4% HCW seropositive for S1/S2 proteins due to national program. Interestingly, 78.4% participants had anti-N protein antibodies, suggesting previous COVID-19 infection. We also evaluated...
The constant evolution of SARS-CoV-2 has provoked the emergence new variants with mutations that evade neutralizing response antibodies. last variant, Omicron, several mutations, which Spike protein is leading cause this evasion. Identifying monoclonal antibodies (mAbs) efficiently neutralize a variant will help generate efficient treatments based on mAbs and reveal unique virus characteristics. In study, we report isolation characterization human antibody called 19n01. This mAb was isolated...
Abstract Background: Previous work showed that the microRNA (miRNA) miR-671-5p was upregulated in monocyte-derived dendritic cells (moDCs) stimulated with Bifidobacterium animalis subsp. lactis BB12 (BB12) no increase IL-10 after six hours of stimulation. In this work, we performed an silico prediction genes targeted by and which are terms pathways involved it. Also, transiently downregulated to assess its effect on regulation. Methods Results: First, a Gene Ontology enrichment analysis...