A5055776492- Autoimmune Neurological Disorders and Treatments
- Ovarian cancer diagnosis and treatment
- Peripheral Neuropathies and Disorders
- Extracellular vesicles in disease
- Endometriosis Research and Treatment
- MicroRNA in disease regulation
- Gestational Diabetes Research and Management
- CRISPR and Genetic Engineering
- Genetic Neurodegenerative Diseases
- RNA regulation and disease
- Uterine Myomas and Treatments
- Prenatal Screening and Diagnostics
- Polyomavirus and related diseases
- Gestational Trophoblastic Disease Studies
- Ectopic Pregnancy Diagnosis and Management
- PARP inhibition in cancer therapy
- Birth, Development, and Health
- Endometrial and Cervical Cancer Treatments
- RNA Research and Splicing
- Hereditary Neurological Disorders
- Cancer, Hypoxia, and Metabolism
- CAR-T cell therapy research
- Genetics, Aging, and Longevity in Model Organisms
- Melanoma and MAPK Pathways
- Polish Law and Legal System
Poznan University of Medical Sciences
2015-2025
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2023-2025
Massachusetts General Hospital
2015-2019
Harvard University
2014-2019
Harvard NeuroDiscovery Center
2015-2018
Wroclaw Medical University
2014
The release of extracellular vesicles (EVs), including exosomes and microvesicles, is a phenomenon shared by many cell types as means communicating with other cells also potentially removing contents. cargo EVs includes the proteins, lipids, nucleic acids, membrane receptors from which they originate. released into space can enter body fluids reach distant tissues. Once taken up neighboring and/or distal cells, transfer functional that may alter status recipient thereby contributing to both...
Abstract Adeno-associated virus (AAV) vectors have shown promising results in preclinical models, but the genomic consequences of transduction with AAV encoding CRISPR-Cas nucleases is still being examined. In this study, we observe high levels integration (up to 47%) into Cas9-induced double-strand breaks (DSBs) therapeutically relevant genes cultured murine neurons, mouse brain, muscle and cochlea. Genome-wide mapping brain shows no overall increase except at CRISPR/Cas9 target site. To...
The APPswe (Swedish) mutation in the amyloid precursor protein (APP) gene causes dominantly inherited Alzheimer's disease (AD) as a result of increased β-secretase cleavage amyloid-β (Aβ) protein. This leads to abnormally high Aβ levels, not only brain but also peripheral tissues carriers. Here, we selectively disrupted human mutant APPSW allele using CRISPR. By applying CRISPR/Cas9 from Streptococcus pyogenes, generated allele-specific deletions either or APPWT. As measured by ELISA,...
Cancer stem cells (CSCs) may contribute to an increased risk of recurrence in ovarian cancer (OC). Further research is needed identify associations between CSC markers and OC patients’ clinical outcomes with greater certainty. If they prove be correct, the future, can used help predict survival indicate new therapeutic targets. This study aimed determine at mRNA protein levels their association presentation, outcome, HGSOC (High-Grade Serous Ovarian Cancer). TCGA (The Genome Atlas) database...
Analysis of cancer-derived extracellular vesicles (EVs) in biofluids potentially provides a source disease biomarkers. At present there is no procedure to systematically identify which antigens should be targeted differentiate from normal host cell-derived EVs. Here, we propose computational framework that integrates information about membrane proteins tumors and tissues databases: UniProt, The Cancer Genome Atlas, the Genotype-Tissue Expression Project, Human Protein Atlas. We developed two...
Gestational diabetes mellitus (GDM) is described as a glucose intolerance of variable severity which begun or was firstly recognized during gravidity. Two major metabolic disorders, insulin resistance and β-cell dysfunction, currently play role in pathogenesis GDM. Our intention to investigate total serum homocysteine vitamin B12 levels pregnant women with GDM non-diabetic gravid women.Serum were prospectively measured 79 women, 60 whom diagnosed GDM, 19 healthy controls. Serum analyzed by...
Do endometrial cancer patients benefit
Extracellular vesicles (EVs) released by cells play a role in intercellular communication. Reporter and targeting proteins can be modified exposed on the surface of EVs to investigate their half-life biodistribution. A characterization membrane-bound Gaussia luciferase (mbGluc) revealed that its signal was detected also form smaller than common (<70 nm). We demonstrated mbGluc initially EVs, likely undergoes proteolytic cleavage processed fragments protein are into extracellular space active...
Visfatin is an adipokine produced and secreted by the adipose tissue. It exerts insulin-like effect insulin receptor-1 has a hypoglycemic effect. We aimed to investigate how serum visfatin changes in women with gestational diabetes mellitus (GDM), whether it predictive of neonatal outcomes.Visfatin levels were prospectively measured peripheral blood enzyme immunoassay 210 pregnant women, 156 which diagnosed GDM, 18 suffered from pregnancy-induced hypertension (PIH) 36 healthy...
Bevacizumab is used in addition to standard, platinum-based chemotherapy treat advanced-stage ovarian cancer patients. Thrombosis a well-documented adverse effect of bevacizumab.The aim this study was identify predictive parameters for thromboembolic events patients and explain how bevacizumab increases the risk these events.Fifty-seven FIGO stage III who underwent cytoreductive surgery were identified included retrospective study. Twenty-six treated with carboplatin paclitaxel (CP) only...
Taurine, a non-proteogenic amino acid and commonly used nutritional supplement, can protect various tissues from degeneration associated with the action of DNA-damaging chemotherapeutic agent cisplatin. Whether how taurine protects human ovarian cancer (OC) cells DNA damage caused by cisplatin is not well understood. We found that OC ascites-derived contained significantly more intracellular than cell culture-modeled OC. In culture, elevation concentration to ascites-cell-associated levels...
Abstract Background Paraneoplastic neurological syndromes (PNS) may coexist with ovarian or lung cancers. Some tumors coexisting PNS are smaller and have a better prognosis than without PNS. constitute an opportunity to observe natural immune antitumor response. We aimed investigate cytotoxic response by measuring granzyme B (GrB) in peripheral blood mononuclear cells (PBMC) patients affected malignancy, accompanying Methods enrolled with: nonmalignant lesions ( n = 21), cancer 19), 57),...
Abstract Introduction Systemic malignant diseases cause the induction of autoimmunity, for example, paraneoplastic syndromes. There are no proofs syndromes in primary brain tumors. The aim study was to evaluate involvement peripheral nervous system, together with an assessment onconeuronal and antineural antibodies as indicators humoral immune response against system patients Materials Methods Clinical examinations, electrophysiological studies nerves (motor sensory conduction velocity...
Endometrial cancer is the most common gynecological worldwide. Classifying endometrial into low- or high-risk groups based on following features recommended: tumor grade, lymphovascular space invasion, myometrial involvement, and non-endometrioid histology. Despite recent progress in molecular profiling of cancer, a substantial group patients are misclassified current criteria. This study aimed to identify proteins that could be used as biomarkers for stratification groups. The proteomic...
Numerous studies suggest that cyclooxygenase-2 (COX-2) is overexpressed in cancer. Our objective was to investigate the relationship between COX-2 expression ovarian carcinoma and clinicopathological factors. An emphasis put on association with new pattern of tumorigenesis divides tumors into type I--less aggressive, II--more aggressive one. The prognostic significance evaluated.Ovarian cancer tissues were obtained from 65 patients FIGO III stage (23 I 42 II cancer). evaluated by...
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