A5072004178- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- Voice and Speech Disorders
- Dysphagia Assessment and Management
- Parkinson's Disease Mechanisms and Treatments
- Zoonotic diseases and public health
- Chemical Looping and Thermochemical Processes
- Hemoglobinopathies and Related Disorders
- Orthopedic Infections and Treatments
- Yersinia bacterium, plague, ectoparasites research
- Hepatitis Viruses Studies and Epidemiology
- Global Maternal and Child Health
- Streptococcal Infections and Treatments
- Pharmacovigilance and Adverse Drug Reactions
- Drug-Induced Adverse Reactions
- Child Nutrition and Feeding Issues
- Burkholderia infections and melioidosis
- Pharmaceutical Quality and Counterfeiting
- Pharmaceutical Economics and Policy
- Bacterial Identification and Susceptibility Testing
- Contact Dermatitis and Allergies
- Malaria Research and Control
Leiden University Medical Center
2022-2023
Instituut voor Tropische Geneeskunde
2015-2019
KU Leuven
2016-2018
Abstract Bloodstream infections by Salmonella enterica serovar Typhimurium constitute a major health burden in sub-Saharan Africa (SSA). These invasive non-typhoidal (iNTS) are dominated isolates of the antibiotic resistance-associated sequence type (ST) 313. Here, we report emergence ST313 sublineage II.1 Democratic Republic Congo. Sublineage exhibits extensive drug resistance, involving combination multidrug extended spectrum β-lactamase production and azithromycin resistance. lineage...
Background. This study reports the microbiological landscape of Salmonella Typhi and invasive nontyphoidal (iNTS) in Democratic Republic Congo (DRC). Methods. Blood cultures obtained from hospital-admitted patients suspected bloodstream infection (BSI) 4 11 provinces DRC (Kinshasa, Bas-Congo, Equateur, Orientale) were processed. Sampling had started 2007; results for period 2011–2014 are reported. Results. iNTS cultured 194 (1.4%) 840 (5.9%), respectively, 14 110 BSI episodes ranked first...
Background Enteric fever remains a major public health problem in low resource settings and antibiotic resistance is increasing. In Asia, an increasing proportion of infections caused by Salmonella enterica serovar Paratyphi A, which for long time was assumed to cause milder clinical syndrome compared Typhi. Methodology A retrospective chart review study conducted 254 unique cases blood culture confirmed enteric who presented at referral adult hospital Phnom Penh, Cambodia between 2008 2015....
Objectives/Hypothesis This study explores whether changes in voice‐ and swallow‐related QoL are associated with progression of idiopathic Parkinson's disease (IPD). Furthermore, it examines the relationship between patients' perception both voice swallowing disorders IPD. Study Design Prospective clinical study, quality life (QoL). Methods One‐hundred mentally competent IPD patients complaints were asked to answer four questionnaires (Voice Handicap Index, MD Anderson Dysphagia Inventory,...
In 2013, an unusual increase in the number of Salmonella enterica serotype Paratyphi A (Salmonella A) infections was reported patients Phnom Penh, Cambodia, and European, American Japanese travellers returning from Cambodia. Epidemiological investigations did not identify a common source exposure. To analyse population structure genetic diversity these isolates, we used whole-genome sequencing on 65 isolates collected 1999 to 2014: 55 acquired Cambodia 10 other countries Asia, Africa Europe....
In sub-Saharan Africa, non-typhoidal Salmonella (NTS) can cause bloodstream infections, referred to as invasive disease (iNTS disease); it occur in outbreaks and is often preceded by malaria. Data from Central Africa limited.Clinical, microbiological molecular findings of NTS recovered a blood culture surveillance project (2009-2014) were analyzed.In March-July 2012 there was an epidemic increase malaria infections the Oriental Province Democratic Republic Congo (DRC). one referral hospital,...
Cambodia has experienced an impressive economic growth in the last two decades that not been matched by equal investments public health care. In combination with other historical and sociocultural factors, this led to a set of private care practices divert from standards clinical good practice. Based on qualitative data collected malaria (para)typhoid fever projects, we will describe falsified laboratory test results, dubious diagnostic both unlicensed licensed doctors, sales substandard...
In daily clinical practice, patients are frequently asked about their swallowing as part of the patient-clinician interview. This study compares diagnostic performance a single open question ‘What swallowing?’ (usual care) with Eating Assessment Tool (EAT-10) reference test in screening for oropharyngeal dysphagia (OD). 303 outpatients at risk OD were recruited three university hospitals: 162 men and 141 women mean age 70 years. All data retrieved by phone. To identify dysphagia, two...
To assess the diagnostic and operational performance of InBiOS AMD rapid test (RDT) (Seattle, USA) for detection B. pseudomallei in grown blood culture broth. The RDT is a lateral flow immunoassay strip format detecting capsular polysaccharide fluids, marketed research only. Broth bottles (BacT/Alert, bioMérieux, Marcy L'Etoile, France) sampled adult patients at Sihanouk Hospital Center HOPE, Phnom Penh, Cambodia, during 2010–2017 stored − 80 °C was tested. They included samples with (n =...
ObjectiveAzithromycin is an alternative to treat invasive non-typhoidal Salmonella (iNTS) infections. We determined its epidemiological cut-off (ECOFF) and compared azithromycin susceptibility testing methods for iNTS.MethodsWe used EUCAST ECOFFinder determine the minimum inhibitory concentrations (MIC; obtained by broth microdilution) ECOFF corresponding disk zone diameters of 515 iNTS from blood cultures in Democratic Republic Congo, Burkina Faso, Rwanda, Cambodia. Transferable resistance...
Background In low resource settings, Salmonella serovars frequently cause bloodstream infections. This study investigated the diagnostic performance of immunochromatographic rapid tests (RDTs), which detect antigens, when applied to stored grown blood culture broth. Material/Methods The SD Bioline One Step Typhi Ag Rapid Detection Kit (Standard Diagnostics, Republic Korea), marketed for detection enterica serovar (Salmonella Typhi) in stool and Test (Creative USA), all serotypes stool, were...
Plasmodium falciparum infection may cause severe anaemia, particularly in children. When planning a diagnostic study on children suspected of malaria sub-Saharan Africa, it was questioned how much blood could be safely sampled; intended volumes (blood cultures and EDTA blood) were 6 mL (children aged <6 years) 10 (6–12 years). A previous review [Bull World Health Organ. 89: 46–53. 2011] recommended not to exceed 3.8 % total volume (TBV). In simulation exercise using data previously enrolled...
Enteric fever is a systemic bacterial infection in humans that endemic Cambodia and for which antibiotic resistance increasingly reported. To guide public health programs, this qualitative study sought to explore community perceptions on transmission treatment. Participant observation was carried out hospital settings, pharmacies, at level Phnom Penh. In-depth interviews 39 one focus group discussion were with blood culture–confirmed enteric patients purposively selected key informants....
Abstract A 23-year-old man presented with a painful swelling of his left shin approximately 5 weeks after very mild trauma. He had not experienced fever, abdominal pain, or diarrhea. Magnetic resonance images were compatible chronic osteomyelitis. The cultures the bone biopsy showed growth Salmonella enterica serovar Enteritidis. Blood remained sterile, there no signs carrier state, and immune deficiency could be found. After surgical debridement, patient was treated ceftriaxone...