A5039705298- Autophagy in Disease and Therapy
- Renal Diseases and Glomerulopathies
- Epigenetics and DNA Methylation
- Chronic Kidney Disease and Diabetes
- Congenital heart defects research
- Vascular Malformations and Hemangiomas
- Cancer-related gene regulation
- Renal and related cancers
- Extracellular vesicles in disease
- Mitochondrial Function and Pathology
- Parkinson's Disease Mechanisms and Treatments
- Cancer, Hypoxia, and Metabolism
- Vascular Malformations Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Endoplasmic Reticulum Stress and Disease
- Genetic and Kidney Cyst Diseases
- RNA Interference and Gene Delivery
- Vascular Anomalies and Treatments
- Ubiquitin and proteasome pathways
- Genetic Syndromes and Imprinting
- Genetics and Neurodevelopmental Disorders
- Reconstructive Surgery and Microvascular Techniques
- Mosquito-borne diseases and control
- Developmental Biology and Gene Regulation
- Reconstructive Facial Surgery Techniques
Wenzhou Medical University
2018-2025
First Affiliated Hospital of Wenzhou Medical University
2018-2025
Zhejiang Lab
2025
University of Maryland, Baltimore
2009-2024
Wuhan University
2011-2022
Hubei Zhongshan Hospital
2021-2022
Union Hospital
2019-2022
Huazhong University of Science and Technology
2019-2022
Hertie Institute for Clinical Brain Research
2013-2021
University of Tübingen
2013-2021
The applications of nanomaterials in regenerative medicine encompass a broad spectrum. functional nanomaterials, such as Prussian blue and its derivative nanoparticles, exhibit potent anti-inflammatory antioxidant properties. By combining it with the corresponding scaffold carrier, fusion biotherapy can be achieved, thereby providing potential avenue for clinical treatment. present study demonstrates fabrication Mesoporous nanoparticles (MPBN) functionalized Inverse Opal Film (IOF)...
Steroid-resistant nephrotic syndrome (SRNS) is a frequent cause of progressive renal function decline and affects millions people. In recent study, 30% SRNS cases evaluated were the result monogenic mutations in 1 27 different genes. Here, using homozygosity mapping whole-exome sequencing, we identified recessive kidney ankyrin repeat-containing protein (KANK1), KANK2, KANK4 individuals with syndrome. an independent functional genetic screen Drosophila cardiac nephrocytes, which are...
Cell-derived microvesicles (MVs), which are biogenic nanosized membrane-bound vesicles that convey bioactive molecules between cells, have recently received attention for use as natural therapeutic platforms. However, the medical applications of MV-based delivery platforms limited by lack effective methods efficient isolation MVs and convenient tuning their targeting properties. Herein, we report development magnetic folate (FA)-modified based on a donor cell-assisted membrane modification...
Abstract Previous studies have implicated that hypoxic stress could enhance osteoclast differentiation; however, the underlying mechanism remains poorly understood. Autophagy is a dynamic lysosomal degradation process has emerged as an important regulator under environment. In present study, we demonstrate for first time autophagy regulates hypoxia‐induced osteoclastogenesis in vitro. We found exposure of RAW264.7 cells to hypoxia (0.2% oxygen) resulted enhanced differentiation, accompanied...
Birth defects are the leading cause of infant mortality, and they caused by a combination genetic environmental factors. Environmental risk factors may contribute to birth in genetically susceptible infants altering critical molecular pathways during embryogenesis, but experimental evidence for gene-environment interactions is limited. Fetal hyperglycemia associated with maternal diabetes results 5-fold increased congenital heart disease (CHD), basis this correlation unknown. Here, we show...
People of African ancestry carrying certain APOL1 mutant alleles are at elevated risk developing renal diseases. However, the mechanisms underlying APOL1-associated diseases unknown. Because gene is unique to humans and some primates, new animal models needed understand function in vivo We generated transgenic Drosophila fly lines expressing human wild type allele (G0) or predominant (G1) different tissues. Ubiquitous expression G0 G1 induced lethal phenotypes, was more toxic than G0....
Histone acetylations are important epigenetic markers for transcriptional activation in response to metabolic changes and various stresses. Using the high-throughput SEquencing-Based Yeast replicative Lifespan screen method yeast knockout collection, we demonstrate that HDA complex, a class-II histone deacetylase (HDAC), regulates aging through its target of acetylated H3K18 at storage carbohydrate genes. We find that, addition longer lifespan, disruption results resistance DNA damage...
Abstract Seeking for effective drugs which are beneficial to facilitating axonal regrowth and elongation after peripheral nerve injury ( PNI ) has gained extensive attention. Fibroblast growth factor 21 FGF 21) is a metabolic that regulates blood glucose lipid homeostasis. However, there little concern the potential protective effect of on regeneration revealing related molecular mechanisms. Here, we firstly found exogenous administration remarkably promoted functional morphologic recovery...
Genomic sequencing has implicated large numbers of genes and de novo mutations as potential disease risk factors. A high throughput in vivo model system is needed to validate gene associations with pathology. We developed a Drosophila -based functional screen candidate identified from Congenital Heart Disease (CHD) patients. 134 were tested the heart using RNAi-based silencing. Quantitative analyses multiple cardiac phenotypes demonstrated essential structural, functional, developmental...
Abstract SARS-CoV-2 infection causes COVID-19, a severe acute respiratory disease associated with cardiovascular complications including long-term outcomes. The presence of virus in cardiac tissue patients COVID-19 suggests this is direct, rather than secondary, effect infection. Here, by expressing individual proteins the Drosophila heart, we demonstrate interaction Nsp6 host MGA/MAX complex (MGA, PCGF6 and TFDP1). Complementing transcriptomic data from fly heart reveal that blocks...
Abstract CD44 is a glycosylated adhesion molecule and osteopontin one of its ligand. undergoes alternative splicing to produce variant isoforms. Our recent studies have shown an increase in the surface expression isoforms (sCD44 v4–v10 CD44) prostate cancer cells over‐expressing (PC3/OPN). Formation CD44/MMP9 complex on cell indispensable for MMP9 activity. In this study, we characterized using RT‐PCR, labeling with NHS–biotin, immunoblotting. Expression encompassing sCD44 at mRNA protein...
Mortalin is an essential component of the molecular machinery that imports nuclear-encoded proteins into mitochondria, assists in their folding, and protects against damage upon accumulation dysfunctional, unfolded aging mitochondria. dysfunction associated with Parkinson's disease (PD) increases vulnerability cultured cells to proteolytic stress leads changes mitochondrial function morphology. To date, Drosophila melanogaster has been successfully used investigate pathogenesis following...
SARS-CoV-2 causes COVID-19 with a widely diverse disease profile that affects many different tissues. The mechanisms underlying its pathogenicity in host organisms remain unclear. Animal models for studying the of proteins are lacking.Using bioinformatic analysis, we found 90% virus-host interactions involve human conserved Drosophila. Therefore, generated series transgenic fly lines individual genes, and used Gal4-UAS system to express these viral genes Drosophila study their...
Many genetic mutations have been identified as monogenic causes of nephrotic syndrome (NS), but important knowledge gaps exist in the roles these genes kidney cell biology and renal diseases. More animal models are needed to assess functions vivo, determine how they cause NS a timely manner. Drosophila nephrocytes human podocytes share striking similarities, what degree known play conserved remains unknown. Here we systematically studied 40 associated with NS, including 7 that not previously...
Studies have identified mutations in >50 genes that can lead to monogenic steroid-resistant nephrotic syndrome (SRNS). The NUP160 gene, which encodes one of the protein components nuclear pore complex nucleoporin 160 kD (Nup160), is expressed both human and mouse kidney cells. Knockdown impairs podocytes cell culture. Recently, siblings with SRNS proteinuria a nonconsanguineous family were found carry compound-heterozygous NUP160.We by whole-exome Sanger sequencing genomic DNA from young...
The pathogenesis of infantile haemangiomas (IHs) is still far from clear despite the fact that they are common vascular tumours distinctive for their perinatal presentation, rapid growth during first year life and subsequent slow involution.To determine role M2-polarised macrophages in IHs.M2-polarised were initially identified 20 specimens IHs by both immunochemistry immunofluorescence CD68 CD163. immunopositive different phases quantified, further analysed correlations with expression...
Background Previous studies have implicated vascular destabilization and changes in extracellular matrix (ECM) composition venous malformations (VMs). Objectives To evaluate the expression levels of connective tissue growth factor (CCN) family matricellular proteins VMs explore their association with destabilization. Methods The CCNs 1–6, transforming (TGF)-β, phosphorylated Tie2 platelet-derived receptor β normal human skin tissues were detected by immunohistochemistry. Correlation between...
ABSTRACT Diabetes is a metabolic disorder characterized by high blood glucose levels and leading cause of kidney disease. Diabetic nephropathy has been attributed to dysfunctional mitochondria. However, many questions remain about the exact mechanism. The structure, function molecular pathways are highly conserved between mammalian podocytes Drosophila nephrocytes; therefore, we used flies on high-sucrose diet model type 2 diabetic nephropathy. nephrocytes from showed significant functional...
miR-1 is a small noncoding RNA molecule that modulates gene expression in heart and skeletal muscle. Loss of Drosophila produces defects somatic muscle embryonic development, which have been partly attributed to directly targeting Delta decrease Notch signaling. Here, we show overexpression the fly wing can paradoxically increase activity independently its effects on Delta. Analyses potential targets revealed regulates 3′UTR E3 ubiquitin ligase Nedd4. Analysis adult Nedd4 protein development...