The composition of bacterial communities in Lake Baikal different hydrological periods and at depths (down to 1515 m) has been analyzed using pyrosequencing the 16S rRNA gene V3 variable region. Most resulting 34 562 reads Bacteria domain have clustered into 1693 operational taxonomic units (OTUs) classified with phyla Proteobacteria, Actinobacteria, Chloroflexi, Bacteroidetes, Firmicutes, Acidobacteria Cyanobacteria. It found that their family level relative contributions distributed over...

There are more than ten mitochondrial genetic codes known in eukaryotes. Genetic code changes also called as codon reassignment may occur through the mechanism including tRNA gene remolding (changing identity mutations anticodon). Our recent study showed that genomes of some amphipod species from Lake Baikal possess genes copies which underwent along with appropriate canonic genes. Three cases were revealed genome Acanthogammarus victorii and by one case shown Eulimnogammarus vittatus...

Phytoplankton is the basis of food web and an indicator environmental change in aquatic ecosystems. assessment uses microscopy, which estimates composition, absolute abundance biomass taxa, metabarcoding, high richness diversity, relative taxa. Problems remain with consistency results from these two methods quantification metabarcoding. Using 18S rRNA metabarcoding microscopy we compared or phytoplankton taxa (class genus/species) south basin Lake Baikal spring over 3 years. Absolute...

Background . The study of recombination processes in the CRISPR-Cas loci Salmonella enterica can help investigate fundamental evolutionary mechanisms system to better understand acquisition phage resistance. aim To proteincoding regions genomes serovars Enteritidis, Infantis, and Typhimurium using silico methods. Materials methods genomic sequences were downloaded from NCBI GenBank database. coding cas genes extracted aligned according codon position. Recombination events identified...

Fragilaria and Ulnaria are two closely related diatom genera for which the delimitation circumscription of several species is unclear. We studied strains isolated from Lake Baikal compared them with freshwater reservoirs in Europe Asia using phylogenetic methods, microscopy interclonal crossing experiments. The results analyses fragments rbcL 18S rRNA genes revealed that baikalian F. radians clade was independent representatives genus other localities. Among we found following tree groups at...

Ural genetic family is a part of the Euro-American lineage Mycobacterium tuberculosis and endemic in Northern Eurasia (former Soviet Union [FSU]). These strains were long described as drug susceptible low virulence, but recent studies reported an increasing circulation multidrug-resistant (MDR) extensively drug-resistant (XDR) strains. Here, we analyzed all publicly available whole genome sequence data genotype isolates, order to elucidate their phylogenomic diversity with special focus on...

Lake Baikal is a unique oligotrophic freshwater lake with unusually cold conditions and amazing biological diversity. Studies of the lake’s viral communities have begun recently, their full diversity not elucidated yet. Here, we performed DNA metagenomic analysis on integral samples from four different deep-water shallow stations southern central basins lake. There was strict distinction in areas environmental conditions. Comparative other lakes revealed highest similarity viromes those...

Lake Baikal was studied as a model for elucidating the general pattern of o-phthalic acid diester (PAE) distributions in surface waters with background pollution levels. The influence factors including congeners, concentrations, sampling points, seasons, years, and potential sources considered environmental risk various hydrobionts established. Priority PAEs are represented by dimethyl phthalate (DMP), diethyl phthalates (DEP), di-n-butyl (DnBP) di-(2-ethylhexyl)phthalate (DEHP)....

We evaluated the temporal signal and substitution rate of tick-borne encephalitis virus (TBEV) using 276 complete open reading frame (ORF) sequences with known collection dates. According to a permutation test, TBEV Siberian subtype (TBEV-S) data set has no structure cannot be applied for estimation without other subtypes. The obtained suggests that common clade (TBEV-common), including all subtypes louping-ill (LIV), is characterized by lowest (1.87 × 10-5 substitutions per site year...

Silica-scaled chrysophytes are a widespread group of microeukaryotes, an important component aquatic habitats. They belong to different evolutionary lineages and they characterized by the presence siliceous scales, but differ in trophic mode. We studied diversity these organisms months ice cover period two subarctic lakes Yakutia, Labynkyr Vorota. chrysophytes, due various modes, have competitive advantage conditions long cover. Statistical analysis has shown relationship between relative...

The measured concentrations of inorganic pollutants, such as ozone (2015–2018), sulfur, and nitrogen oxides (2012–2018) at air monitoring sites in the south Eastern Siberia were sampled, following passive sampling method, analyzed. spatial inhomogeneity atmospheric gas is presented. concentration lower urban areas than those rural background level. However, sulfur oxide are higher atmosphere over city site. seasonal dependence was determined using its maximum (March–April) minimum...

The present study examined the taxonomic diversity of silica-scaled chrysophytes in estuaries Arctic watershed Yakutia context global climate change, as these aquatic organisms are highly sensitive to environmental changes. Previously, 41 species were recorded waters Yakutia. In study, we supplemented this list with 55 species. We observed a high richness (82 taxa) area. Of these, eight Russia for first time. At present, area represents northernmost habitat most during study. rivers is...

In this study, we analysed the diversity and composition of double-stranded DNA viral bacterial communities within sample surface coastal water Southern Baikal through metagenomics deep sequencing 16S ribosomal RNA gene, respectively. The rRNA gene analysis has revealed 14 phyla dominance 'Actinobacteria' (43.6%), 'Proteobacteria' (25.2%) 'Bacteroidetes' (11.5%). was similar to that obtained previously in Lake littoral zone. Out 1 030 169 processed virome reads, 37.4% sequences (385 421)...

Until 2020, there were only three original complete genome (CG) nucleotide sequences of Omsk hemorrhagic fever virus (OHFV) in GenBank. For this reason, the evolutionary rate and divergence time assessments reported literature based on E gene sequences, but notably without temporal signal evaluation, such that their reliability is unclear. As July 2022, 47 OHFV CG have been published, which enables testing data inferring unbiased reliable substitution values. Regression analysis TempEst...

There are more than 350 species of amphipods (Crustacea) in Lake Baikal, which have emerged predominantly through the course endemic radiation. This group represents a remarkable model for studying various aspects evolution, one is evolution mitochondrial (mt) genome architectures. We sequenced and assembled mt pelagic Baikalian amphipod Macrohectopus branickii. The revealed to an extraordinary length (42,256 bp), deviating significantly from genomes other majority animals. M. branickii has...

In this study, we present results on fatty acid analysis of phytoplankton Lake Baikal, the world’s deepest lake, which differs from other lakes by its oceanic features. Since used a large-mesh net, net sample were primarily represented large elongated diatom Synedra acus. subsp. radians (Kützing) Skabichevskij. The similar algae composition samples spring season collected at different sites lake allows us to compare these samples. diversity sedimentation was contrary 32 species. There are...

Sponges (type Porifera) are multicellular organisms that give shelter to a variety of microorganisms: fungi, algae, archaea, bacteria, and viruses. The studies concerning the composition viral communities in sponges have appeared rather recently, diversity role viruses sponge holobionts remain largely undisclosed. In this study, we assessed DNA associated community Baikal endemic sponge, Baikalospongia bacillifera, using metagenomic approach, compared virome data from samples water (control...