A5032946856- Mitochondrial Function and Pathology
- Microtubule and mitosis dynamics
- Cancer Cells and Metastasis
- Plant biochemistry and biosynthesis
- Natural product bioactivities and synthesis
- Epigenetics and DNA Methylation
- Advanced Fluorescence Microscopy Techniques
- Chemotherapy-induced cardiotoxicity and mitigation
- Helminth infection and control
- 3D Printing in Biomedical Research
- Cancer, Hypoxia, and Metabolism
- Berberine and alkaloids research
- Parasite Biology and Host Interactions
- Cardiac Ischemia and Reperfusion
- Carbon Nanotubes in Composites
- ATP Synthase and ATPases Research
- Cell death mechanisms and regulation
- RNA Research and Splicing
- Bioactive Compounds and Antitumor Agents
- Genetic and phenotypic traits in livestock
- Ion channel regulation and function
- Pluripotent Stem Cells Research
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Neuroscience and Neural Engineering
- Urticaria and Related Conditions
University of Minnesota, Duluth
2010-2025
Duluth Clinic
2021
University of Minnesota Medical Center
2018
University of Minnesota
1998-2015
University of Minnesota System
2003
Mount Desert Island Biological Laboratory
1997
University of Wisconsin System
1994
University of Wisconsin–Madison
1987-1991
Thomas Jefferson University
1989
Charles University
1975
In the present work, lipophilic caffeic and ferulic acid derivatives were synthesized, their cytotoxicity on cultured breast cancer cells was compared. A total of six compounds initially evaluated: (CA), hexyl caffeate (HC), caffeoylhexylamide (HCA), (FA), ferulate (HF), feruloylhexylamide (HFA). Cell proliferation, cell cycle progression, apoptotic signaling investigated in three human lines, including estrogen-sensitive (MCF-7) insensitive (MDA-MB-231 HS578T). Furthermore, direct...
The invasive nature of cancer cell lines is thought to correlate with their metastatic potential. Most traditional assays, however, do not examine these features in a three-dimensional environment and the resulting data suffer from reduced biological applicability. Here an approach presented visualize ability physiologically relevant setting. spheroid invasion assay first utilizes gravity generate spheroids within drops media that hang lid culture dish. Next, are embedded 3D matrix...
Berberine [Natural Yellow 18, 5,6-dihydro-9,10-dimethoxybenzo(<i>g</i>)-1,3-benzodioxolo(5,6-<i>a</i>)quinolizinium] is an alkaloid present in plant extracts and has a history of use traditional Chinese Native American medicine. Because its ability to arrest the cell cycle cause apoptosis several malignant lines, it received attention as potential anticancer therapeutic agent. Previous studies suggest that mitochondria may be important target berberine, but relatively little known about...
Ovarian cancer is a complex disease marked by tumor heterogeneity, which contributes to difficulties in diagnosis and treatment. New molecular targets better profiles defining subsets of patients are needed. tRNA fragments (tRFs) offer recently identified group noncoding RNAs that often as abundant microRNAs cells. Initially their presence deep sequencing data sets was attributed the breakdown mature tRNAs, however, it now clear they actively generated function multiple regulatory events....
When exposed to oxidative conditions, cells suffer not only biochemical alterations, but also morphologic changes. Oxidative stress is a condition induced by some pro-oxidant compounds, such as tert-butylhydroperoxide (tBHP) and can be in vivo ischemia/reperfusion which very common cardiac tissue. The cell line H9c2 has been used an vitro cellular model for both skeletal muscle. Understanding how these respond agents may furnish novel insights into tissues conditions. objective of this work...
The goal of this investigation was to determine whether chenodeoxycholic acid (CDCA)-induced apoptosis is prevented by ursodeoxycholic (UDCA) or tauroursodeoxycholic (TUDC) and characterize the involvement mitochondria in process. Cultured human HepG2 cells were treated a dose- time-dependent protocol order establish sufficiently low exposure CDCA that causes but not necrosis. Low-dose induced an S-phase block G2 arrest cell cycle, as determined flow cytometry. As result, proliferation...
Abstract Background The quaternary isoquinoline alkaloid sanguinarine is receiving increasing attention as a potential chemotherapeutic agent in the treatment of cancer. Previous studies have shown that this DNA-binding phytochemical can arrest number different types transformed cells G0/G1, and upregulate CKIs p21 p27 while downregulating multiple cyclins CDKs. To more closely examine responses some these cell cycle regulatory molecules to sanguinarine, we used immunocytochemical methods...
Potent and dual monocarboxylate transporter (MCT) 1 4 inhibitors have been developed for the first time as potential anticancer agents based on α-cyanocinnamic acid structural template. Candidate 1-9 evaluated in vitro cell proliferation against MCT1 MCT4 expressing cancer lines. Potential binding interactions of lead compound 9 studied through homology modeling molecular docking prediction. In effects extracellular flux via glycolysis mitochondrial stress tests suggest that candidate...
Abstract Curcumin is a dietary phytochemical associated with anti‐tumorigenic effects, but the mechanisms by which it inhibits cancer cell growth and metastasis are not completely understood. For example, little information available regarding effects of curcumin on cytoskeletal organization function. In this study, time‐lapse video immunofluorescence labeling methods were used to demonstrate that significantly alters microfilament motility in PC‐3 LNCaP human prostate cells vitro. rapidly...
The objective of this study was to analyze the immune response mice larval stages Brugia malayi. Male BALB/c were inoculated with 3 doses irradiated third-stage larvae (L-3) B. malayi and subsequently challenged L-3 implanted ip within diffusion chambers. After weeks, recovered determine their viability, length, stage development. A significant reduction in parasite survival observed immunized mice. Furthermore, from significantly shorter than control All L-3, whereas 96% controls...
ABSTRACT Most studies using intrinsic NAD(P)H as biomarkers for energy metabolism and mitochondrial anomalies have been conducted in routine two‐dimensional (2D) cell culture formats. Cellular behavior, however, can be significantly different 2D cultures from that vivo. As a result, there are emerging interests integrating noninvasive, quantitative imaging techniques of with vivo‐like three‐dimensional (3D) models. The overall features metabolic responses the murine breast cancer cells line...
Given the relatively long life of stem cells (SCs), efficient mechanisms quality control to balance cell survival and resistance external internal stress are required. Our objective was test relevance for SCs maintenance, differentiation death. We compared in P19 (P19SCs) before after (P19dCs). Differentiation P19SCs resulted alterations parameters involved protein homeostasis, including redox system, cardiolipin lipid profiles, unfolded response, ubiquitin-proteasome lysosomal systems,...
This article studies the adhesion, growth and differentiation of stem cells on carbon nanotube matrices. Glass coverslips were coated with multi-walled (MWNT) thin films using layer-by-layer self-assembling techniques. Pluripotent P19 mouse embryonal carcinoma seeded onto uncoated or MWNT-coated glass either maintained in an undifferentiated state induced to differentiate by addition retinoic acid. The authors found that cell adhesion was increased surfaces, expression patterns some markers...