A5022559731- Aquaculture Nutrition and Growth
- Metabolomics and Mass Spectrometry Studies
- Aquaculture disease management and microbiota
- Mitochondrial Function and Pathology
- Diet and metabolism studies
- Physiological and biochemical adaptations
- Diet, Metabolism, and Disease
- Metabolism and Genetic Disorders
- Liver Disease Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Glycogen Storage Diseases and Myoclonus
- Autophagy in Disease and Therapy
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Advanced MRI Techniques and Applications
- Lipid metabolism and biosynthesis
- Birth, Development, and Health
- Sirtuins and Resveratrol in Medicine
- Meat and Animal Product Quality
- Pregnancy and preeclampsia studies
- Epigenetics and DNA Methylation
- Fish biology, ecology, and behavior
- Reproductive biology and impacts on aquatic species
- ATP Synthase and ATPases Research
- MRI in cancer diagnosis
University of Coimbra
2014-2025
Escola Universitária Vasco da Gama
2021-2025
Centro de Neurociências e Biologia Celular
2022
Biocant
2016-2021
Non-alcoholic fatty liver disease (NAFLD) is a health concern affecting 24% of the population worldwide. Although pathophysiologic mechanisms underlying are not fully clarified, mitochondrial dysfunction and oxidative stress key players in progression. Consequently, efforts to develop more efficient pharmacologic strategies targeting mitochondria for NAFLD prevention/treatment underway. The conjugation caffeic acid anti-oxidant moiety with an alkyl linker triphenylphosphonium cation (TPP+),...
The de novo synthesis of triglyceride (TG) fatty acids (FA) and glycerol can be measured with stable isotope tracers. However, these methods typically do not inform the contribution a given substrate to specific pathways on synthetic processes. We integrated deuterated water (2H2O) measurement lipogenesis (DNL) glycerol-3-phosphate (GLY) from all substrates 13C nuclear magnetic resonance (NMR) method that quantifies TG FA enrichment [U-13C]precursor. This allowed [U-13C]precursor DNL GLY...
// Rute Loureiro 1 , Silvia Magalhães-Novais 1, 2 Katia A. Mesquita Ines Baldeiras 3 Isabel S. Sousa Ludgero C. Tavares Barbosa Paulo J. Oliveira Ignacio Vega-Naredo 4 CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Portugal Department Life Sciences, School Medicine, Morphology Oviedo, Spain Correspondence to: Vega-Naredo, e-mail: naredo@gmail.com Keywords: melatonin, mitochondria, metabolism, dichloroacetate, cancer stem cells Received: January 19, 2015 Accepted: May...
Early life malnutrition results in structural alterations the kidney, predisposing offspring to later renal dysfunction. Kidneys of adults who were growth restricted at birth have substantial variations nephron endowment. Animal models indicated and functional consequences exposed suboptimal intrauterine nutrition. Mitochondrial bioenergetics play a key role energy metabolism, growth, function. We hypothesized that moderate maternal nutrient reduction (MNR) would adversely impact fetal...
Abstract Poor maternal nutrition in pregnancy affects fetal development, predisposing offspring to cardiometabolic diseases. The role of mitochondria during development on later-life cardiac dysfunction caused by nutrient reduction (MNR) remains unexplored. We hypothesized that MNR gestation causes bioenergetic deficits, compromising mitochondrial metabolism and reserve capacity. To enable human translation, we developed a primate baboon model (Papio spp.) moderate which mothers receive 70%...
The replacement of fishmeal in aquafeeds is essential to the sustainability aquaculture. Besides procurement alternative protein sources, fish can also be selected for better performance on plant-based diets. Rainbow trout (Oncorhynchus mykiss) one such species which strain ARS-Sel has been higher growth and enhanced utilization when fed soy-based aim this study was compare plasma digesta metabolomes between two commercial strains (CS-1 CS-2), plant-protein (PM) fishmeal-based (FM) diets,...
Deuterated water (2H2O) is widely used for measuring de novo lipogenesis (DNL). 2H incorporated into fatty acids via exchange between body and the hydrogens of acetyl-CoA, malonyl-CoA, NADPH. Previous studies concluded that these exchanges are incomplete; therefore, acid enrichment requires correcting. In mice, we measured positions 2 3 methyl from [U-2H7]glucose to determine transfer glucose NADPH, respectively. Positional enrichments were compared with 13C same sites an equivalent amount...
Background: In MASLD (formerly called NAFLD) mouse models, oversupply of dietary fat and sugar is more lipogenic than either nutrient alone. Fatty acids suppress de novo lipogenesis (DNL) from sugars, while DNL inhibits fatty acid oxidation. How such factors interact to impact hepatic triglyceride levels are incompletely understood. Methods: Using deuterated water, we measured in mice fed 18-weeks with standard chow (SC), SC supplemented 55/45-fructose/glucose the drinking water at 30% (w/v)...
The sustainable growth of fish aquaculture will require the procurement non-marine feed sources. Glycerol is a potential supplement whose metabolism may spare catabolism dietary amino acids, thereby extending use protein to other physiological functions such as growth. In present study, effects glycerol supplementation on muscle and liver metabolomes rainbow trout (Oncorhynchus mykiss) European seabass (Dicentrarchus labrax) were evaluated. Fish juveniles fed diets with 0%, 2.5%, 5%...
Practical diets for commercial barramundi production rarely contain greater than 10% starch, used mainly as a binding agent during extrusion. Alternative ingredients such digestible starch have shown some capacity to spare dietary protein catabolism generate glucose. In the present study, carnivorous fish species, Asian seabass (Lates calcarifer) was subjected two with same energy: Protein (P) - high content (no starch); and Starch (S) (pregelatinized) content. The effects of diet on hepatic...
Abstract Barramundi ( Lates calcarifer ) are a highly valued aquaculture species, and, as obligate carnivores, they have demonstrated preference for dietary protein over lipid or starch to fuel energetic growth demands. In order investigate how carnivorous fish regulate nutritional cues, we examined the metabolic effects of feeding two isoenergetic diets that contained different proportions digestible energy. Fish fed high proportion energy had higher liver SFA, but showed no change in...
Intra-uterine growth restriction (IUGR) is a common cause of fetal/neonatal morbidity and mortality associated with increased offspring predisposition for cardiovascular disease (CVD) development. Mitochondria are essential organelles in maintaining cardiac function, thus, fetal mitochondria could be responsive to the IUGR environment. In this study, we investigated whether utero mitochondrial programming can detectable an early stage pregnancy. Using well-established nonhuman primate model,...
13 C NMR isotopomer analysis was used to characterize intermediary metabolism in three colorectal cancer cell lines (WiDr, LS1034, and C2BBe1) determine the “metabolic remodeling” that occurs under hypoxia. Under normoxia, present high rates of lactate production can be seen as “Warburg” like cells independently substrate availability, since such profile dominant at both low glucose media contents. The LS1034 less glycolytic most affected by event hypoxia, raising abruptly consumption...
Dietary glucose and fructose are both efficiently assimilated by the liver but a comprehensive measurement of this process starting from their conversion to sugar phosphates, involvement pentose phosphate pathway (PPP), glycogen lipid storage products, remains incomplete. Mice were fed chow diet supplemented with 35 g/100 mL drinking water 55/45 fructose/glucose mixture for 18 weeks. On final night, was enriched either [U-13C]glucose or [U-13C]fructose, deuterated (2H2O) also administered....
The pharmacological interest in mitochondria is very relevant since these crucial organelles are involved the pathogenesis of multiple diseases, such as cancer. In order to modulate cellular redox/oxidative balance and enhance mitochondrial function, numerous polyphenolic derivatives targeting have been developed. Still, due drug resistance emergence several cancer therapies, significant efforts being made develop drugs that combine induction metabolic reprogramming with ability generate...
Inflammatory bowel disease is characterized by chronic relapsing idiopathic inflammation of the gastrointestinal tract and persistent inflammation. Studies focusing on immune-regulatory function reactive oxygen species (ROS) are still largely missing. In this study we analysed a ROS-deficient mouse model leading to colon adenocarcinoma. Colitis was induced with dextran sulfate sodium (DSS) supplied via drinking water in wild-type (WT) Ncf1-mutant (Ncf1) B10.Q mice using two different...