A5062321417- Cellular transport and secretion
- Sperm and Testicular Function
- Sexual Differentiation and Disorders
- Angiogenesis and VEGF in Cancer
- Urological Disorders and Treatments
- Lymphatic System and Diseases
- HER2/EGFR in Cancer Research
- Cell Adhesion Molecules Research
- S100 Proteins and Annexins
- Axon Guidance and Neuronal Signaling
- Reproductive Biology and Fertility
- Monoclonal and Polyclonal Antibodies Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Renal and related cancers
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Multiple Myeloma Research and Treatments
- Apelin-related biomedical research
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Genetic and Kidney Cyst Diseases
- Protease and Inhibitor Mechanisms
- Genomics and Chromatin Dynamics
- Glycosylation and Glycoproteins Research
- Bone Metabolism and Diseases
Aichi Gakuin University
2024-2025
Kansai Medical University
2019-2022
Vanderbilt University
2018-2019
Ehime University
2009-2016
Kumamoto University
2008-2011
Center for Agricultural Resources Research
2008
Molina Center for Energy and the Environment
2008
Abstract The exocyst is a conserved octameric complex that tethers exocytic vesicles to the plasma membrane prior fusion. Exocyst assembly and delivery mechanisms remain unclear, especially in mammalian cells. Here we tagged multiple endogenous subunits with sfGFP or Halo using Cas9 gene-editing, create single double knock-in lines of mammary epithelial cells, interrogated dynamics by high-speed imaging correlation spectroscopy. We discovered comprised tetrameric subcomplexes can associate...
The epididymis is a male accessory organ and functions for sperm maturation storage under the control of androgen. development also androgen dependent. Wolffian duct (WD), anlagen epididymis, formed in both female embryos; however, it stabilized only embryos by testicular Androgen drives subsequent differentiation WD into epididymis. Although essential roles masculinization epididymal function have been established, little known about cellular events regulated precisely signaling during...
Class 3 semaphorins are axon guidance factors implicated in tumor and vascular biology, including invasive activity. Recent studies indicate that semaphorin 3F (SEMA3F) is a potent inhibitor of metastasis; however, its functional role breast cancer not fully understood. We found exogenous SEMA3F inhibited phosphorylation Akt mTOR downstream kinase S6K MDA-MB-231 MCF7 cells via neuropilin-2 (NRP2) receptor. also examined the effect on progression vivo allograft model. The mouse 4T1 or...
Split hand/foot malformation (SHFM) is syndromic ectrodactyly often associated with mental retardation and/or craniofacial defects. Several clinical reports previously described urogenital dysplasia such as micropenis, hypospadias, and small testis in SHFM patients. Genetic lesions the Dlx5 Dlx6 (Dlx5/6) locus are human genetic disorder type 1. Although Dlx5/6 expressed testis, their possible function of during differentiation has not been described. In this study, we show that fetal Leydig...
A disintegrin and metalloproteinase (ADAM) is a family of enzymes involved in ectodomain shedding various membrane proteins. However, the molecular mechanism underlying substrate recognition by ADAMs remains unknown. In this study, we successfully captured analyzed cell surface transient assemblies between transmembrane amphiregulin precursor (proAREG) ADAM17 during an early phase, which enabled identification annexins as components their complex. Annexin members annexin A2 (ANXA2), A8, A9...
Abstract Regulating the amount of proteins in living cells is a powerful approach for understanding functions proteins. Immunomodulatory drugs (IMiDs) induce degradation neosubstrates by interacting with celebron (CRBN) cullin E3 ubiquitin ligase complex (CRL4 CRBN ). Here, we developed IMiD-dependent Sal-like protein 4 (SALL4) degron (S4D) system chemical knockdown. In transient assays, an N- or C-terminal S4D tag induced localized to various subcellular compartments, including plasma...
ABSTRACT Androgen plays a crucial role in initiating and maintaining the expression of male sexual characteristics mammals. In humans mice, any defects along pathway androgen functions result congenital urogenital abnormalities. The genital tubercle (GT), an anlage external genitalia, differentiates into penis males clitoris females. Although masculinization genitalia is androgen‐dependent, molecular its potential downstream genes largely unclear. To identify involved mouse GT...
Epithelial cell plasticity is controlled by extracellular cues, but the underlying mechanisms remain to be fully understood. Epidermal growth factor (EGF) and amphiregulin (AREG) are high- low-affinity ligands for EGF receptor (EGFR), respectively. EGFR signaling known promote epithelial-mesenchymal transition (EMT) activation of ERK induction an EMT transcription factor, ZEB1. Here, we demonstrate that ligand-switching between AREG at equivalent molarity reversibly interconverts epithelial...
HASPIN is a serine/threonine kinase that regulates mitosis by phosphorylating histone H3 at threonine 3. The expression levels of in various cancers are associated with tumor malignancy and poor survival, suggesting inhibition may suppress cancer growth. As mRNA elevated human breast tissues compared adjacent normal tissues, we examined the growth-suppressive effects CHR-6494, potent inhibitor, cell lines vitro vivo . We found was expressed cells all molecular subtypes, as well immortalized...
The exocyst is a conserved octameric complex that tethers exocytic vesicles to the plasma membrane prior fusion. Exocyst assembly and delivery mechanisms remain unclear, especially in mammalian cells. Here we tagged multiple endogenous subunits with sfGFP or Halo using Cas9 gene editing, create single double knock-in lines of mammary epithelial cells, interrogated dynamics by high-speed imaging correlation spectroscopy. We discovered comprised tetrameric subcomplexes that, unexpectedly, can...
HASPIN acts in chromosome segregation via histone phosphorylation. Recently, inhibitors have been shown to suppress growth of various cancer cells. Pancreatic has no symptom the early stages and may progress before detection. So, 5-year survival rate is low. Here, we reported that administration inhibitor, CHR-6494, mice bearing pancreatic BxPC-3-Luc cells significantly suppressed CHR-6494 might be a useful agent for treating cancer.