A5007146552- Neurogenesis and neuroplasticity mechanisms
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- HER2/EGFR in Cancer Research
- MicroRNA in disease regulation
- Axon Guidance and Neuronal Signaling
- Angiogenesis and VEGF in Cancer
- S100 Proteins and Annexins
- Pluripotent Stem Cells Research
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Proteoglycans and glycosaminoglycans research
- Cell death mechanisms and regulation
- Immune Response and Inflammation
- Wnt/β-catenin signaling in development and cancer
- Fibroblast Growth Factor Research
- Tissue Engineering and Regenerative Medicine
- bioluminescence and chemiluminescence research
- Immune Cell Function and Interaction
- Protein Kinase Regulation and GTPase Signaling
- Gut microbiota and health
- Melanoma and MAPK Pathways
- Wound Healing and Treatments
Aichi Gakuin University
2021-2025
Ehime University
2011-2022
Vanderbilt University Medical Center
2020-2021
RELX Group (United States)
2017
RIKEN Center for Integrative Medical Sciences
2009-2014
Yokohama City University
2009-2014
Keio University
2014
RIKEN
2012
Kumamoto University
2003-2007
Fc receptors specifically bind to the region of Igs mediate unique functions each class Igs. To identify a novel receptor for IgM, we searched expressed sequence tag database molecules containing Ig domains with homology those known Fcα/μR and polymeric receptor. As result, identified TOSO/Fas apoptotic inhibitory molecule 3 (FAIM3) as possible IgM. HeLa cells transfected TOSO/FAIM3-expression vector bound IgM but not IgG were able internalize IgM-conjugated beads IgG-conjugated beads,...
Astrocytes play important roles in brain development and injury response. Transcription factors STAT3 Smad1, activated by leukemia inhibitory factor (LIF) bone morphogenetic protein 2 (BMP2), respectively, form a complex with the coactivator p300 to synergistically induce astrocytes from neuroepithelial cells (NECs) (K. Nakashima, M. Yanagisawa, H. Arakawa, N. Kimura, T. Hisatsune, Kawabata, K. Miyazono, Taga, Science 284:479-482, 1999). However, mechanisms that govern astrogliogenesis...
Abstract Trehalose is the nonreducing disaccharide of glucose, evolutionarily conserved in invertebrates. The living skin equivalent (LSE) an organotypic coculture containing keratinocytes cultivated on fibroblast-populated dermal substitutes. We demonstrated that human primary fibroblasts treated with highly concentrated trehalose promote significantly extensive spread epidermal layer LSE without any deleterious effects. RNA-seq analysis trehalose-treated 2D and 3D at early time points...
Cutaneous hyaluronan (HA) is depolymerized to intermediate sizes in the extracellular matrix, and further fragmented regional lymph nodes. Previously, we showed that HA-binding protein involved HA depolymerization (HYBID), also known as KIAA1199/CEMIP, responsible for first step of depolymerization. Recently, mouse transmembrane 2 (mTMEM2) with high structural similarity HYBID was proposed be a membrane-bound hyaluronidase. However, knockdown human TMEM2 (hTMEM2) conversely promoted normal...
Class 3 semaphorins are axon guidance factors implicated in tumor and vascular biology, including invasive activity. Recent studies indicate that semaphorin 3F (SEMA3F) is a potent inhibitor of metastasis; however, its functional role breast cancer not fully understood. We found exogenous SEMA3F inhibited phosphorylation Akt mTOR downstream kinase S6K MDA-MB-231 MCF7 cells via neuropilin-2 (NRP2) receptor. also examined the effect on progression vivo allograft model. The mouse 4T1 or...
Significance The tumor suppressive function of p53 is tightly regulated by its posttranslational modifications. Although Ser46 phosphorylation a critical modification for apoptosis induction, molecular mechanism which Ser46-phosphorylated induces remains unclear. Here, we clarify that amphiregulin (AREG) specifically induced in phosphorylation-specific manner. Notably, AREG colocalizes with DEAD-box RNA helicase p68 (DDX5) the nucleus and regulates microRNA biogenesis response to DNA damage....
A disintegrin and metalloproteinase (ADAM) is a family of enzymes involved in ectodomain shedding various membrane proteins. However, the molecular mechanism underlying substrate recognition by ADAMs remains unknown. In this study, we successfully captured analyzed cell surface transient assemblies between transmembrane amphiregulin precursor (proAREG) ADAM17 during an early phase, which enabled identification annexins as components their complex. Annexin members annexin A2 (ANXA2), A8, A9...
The acidic (leucine-rich) nuclear phosphoprotein 32 family member B (ANP32B), a highly conserved of the (ANP32) family, is critical for development normal tissue. However, its role in hepatocellular carcinoma (HCC) controversial. In this study, we elucidated ANP32B HCC cell lines and tissues. expression was modulated using siRNA plasmids lentiviruses. levels apoptosis-related proteins were analyzed by real-time RT-PCR Western blotting. tissues from patients with investigated...
Abstract Basic fibroblast growth factor (bFGF) is commonly used to enrich and maintain neural stem cells in vitro. Olig2 an essential transcription for oligodendrocyte lineage specification expressed predominantly ventral neuroepithelial the medial lateral ganglionic eminence (GE), where progenitors originate. Here we report significant induction of expression dorsal neuroepithelium‐derived cultured presence bFGF, which Olig2‐expressing were initially negligible. Among appearing after a...
Amphiregulin ( AR ) is derived from a membrane‐anchored form (pro by ectodomain shedding, and ligand that activates epidermal growth factor receptor EGFR ). We have recently shown pro translocates the plasma membrane to nucleus after truncation of 11 amino acids at C ‐terminus, which independent conventional signaling pathway. Although immunoreactivity has reportedly been detected in cancer cells, its biological meaning never investigated. This study was performed investigate roles nuclear...
Membrane-anchored heparin-binding epidermal growth factor-like factor (proHB-EGF) yields soluble HB-EGF, which is an receptor (EGFR) ligand, and a carboxy-terminal fragment of HB-EGF (HB-EGF-CTF) after ectodomain shedding. We previously reported that HB-EGF-CTF unshed proHB-EGF has the cytoplasmic domain (HB-EGF-C), translocate from plasma membrane to nucleus regulate cell cycle shedding stimuli. However, significance nuclear exported HB-EGF-C in human gastric cancer unclear. investigated...
Epithelial cell plasticity is controlled by extracellular cues, but the underlying mechanisms remain to be fully understood. Epidermal growth factor (EGF) and amphiregulin (AREG) are high- low-affinity ligands for EGF receptor (EGFR), respectively. EGFR signaling known promote epithelial-mesenchymal transition (EMT) activation of ERK induction an EMT transcription factor, ZEB1. Here, we demonstrate that ligand-switching between AREG at equivalent molarity reversibly interconverts epithelial...
To understand host gastrointestinal response after exposure to probiotic Lactobacillus acidophilus L-92, microarray analysis of cultured epithelial Caco-2 cells was performed. Of the 187 genes down-regulated 4 h treatment with 25 were involved in RNA splicing; 12, cell cycle; 8 transcriptional regulators; 2 ubiquitin proteolysis; 2, adhesion; meiosis; and encoding cytokines. In splicing group, small nuclear RNAs, pore complex interacting proteins, binding motif SMG1 homologs...
Understanding the mechanism of tumor necrosis factor (TNF)-α shedding is important because TNF-α triggers inflammatory bowel disease development. A disintegrin and metalloproteinase (ADAM) 17 a key enzyme for not only type 1 membrane-anchored protein, amphiregulin, but also 2 TNF-α. However, detailed by which ADAM17 cleaves proteins unclear. Annexin (ANX) A2 involved in ADAM17-mediated amphiregulin shedding. In this study, we examined whether ANX We prepared U937, HT29, HCT116 cells...
An asymmetric synthesis of two analogues SL0101 (1) has been achieved. The effort is aimed at the discovery inhibitors p90 ribosomal S6 kinase (RSK) with improved bioavailability. route relies upon use Taylor catalyst to regioselectively install C-3″ acetyl or carbamate functionality. This study led identification a third-generation analogue C-4″ n-Pr-carbamate and acetate RSK inhibitory activity.