A5011980870- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Thyroid Cancer Diagnosis and Treatment
- Photodynamic Therapy Research Studies
- Medical Imaging and Pathology Studies
- Cancer Cells and Metastasis
- Ovarian cancer diagnosis and treatment
- Cell Image Analysis Techniques
- Molecular Biology Techniques and Applications
- AI in cancer detection
- Thyroid and Parathyroid Surgery
- Lung Cancer Diagnosis and Treatment
- Advanced Biosensing Techniques and Applications
- Atomic and Subatomic Physics Research
- Radiomics and Machine Learning in Medical Imaging
- Cellular Mechanics and Interactions
- Ultrasound Imaging and Elastography
- Spectroscopy Techniques in Biomedical and Chemical Research
- Health and Medical Research Impacts
- Connective tissue disorders research
- Nanoplatforms for cancer theranostics
- Single-cell and spatial transcriptomics
- Thyroid Disorders and Treatments
- Cervical Cancer and HPV Research
Vrije Universiteit Amsterdam
2019-2024
Amsterdam University Medical Centers
2020-2023
Amsterdam UMC Location Vrije Universiteit Amsterdam
2023
Netherlands Institute for Radio Astronomy
2020
During lung cancer operations a rapid and reliable assessment of tumor tissue can reduce operation time potentially improve patient outcomes. We show that third harmonic generation (THG), second (SHG) two-photon excited autofluorescence (2PEF) microscopy reveals relevant, histopathological information within seconds in fresh unprocessed human samples. used compact, portable microscope recorded images 1 to 3 using power 5 mW. The generated THG/SHG/2PEF tumorous nontumorous tissues are...
Distinguishing tumors from normal brain cells is important but challenging in glioma surgery due to the lack of clear interfaces between two. The ability label-free third harmonic generation (THG) microscopy combination with automated image analysis quantitatively detect infiltration fresh, unprocessed tissue real time assessed. THG images reveal increased cellularity grades II-IV samples 23 patients, as confirmed by subsequent hematoxylin and eosin histology. An quantification workflow...
Real‐time assessment of excised tissue may help to improve surgical results in breast tumor surgeries. Here, as a step towards this purpose, the potential second and third harmonic generation (SHG, THG) microscopy is explored. SHG THG are nonlinear optical microscopic techniques that do not require labeling generate 3D images with intrinsic depth‐sectioning at sub‐cellular resolution. Until now, technique had been applied on fixated or visualize stroma only, whereas most tumors start lobules...
During thyroid surgery fast and reliable intra-operative pathological feedback has the potential to avoid a two-stage procedure significantly reduce health care costs in patients undergoing diagnostic hemithyroidectomy (HT). We explored higher harmonic generation (HHG) microscopy, which combines second (SHG), third (THG), multiphoton excited autofluorescence (MPEF) for this purpose. With compact, portable HHG microscope, images of freshly excised healthy tissue, benign nodules (follicular...
"Rapid On-Site Pathology Visualization of COVID-19 Characteristics Using Higher Harmonic Generation Microscopy." American Journal Respiratory and Critical Care Medicine, 0(ja), pp.
In diseases such as interstitial lung (ILDs), patient diagnosis relies on diagnostic analysis of bronchoalveolar lavage fluid (BALF) and biopsies. Immunological BALF includes differentiation leukocytes by standard cytological techniques that are labor-intensive time-consuming. Studies have shown promising leukocyte identification performance blood fractions, using third harmonic generation (THG) multiphoton excited autofluorescence (MPEF) microscopy.To extend to samples THG/MPEF microscopy,...
Abstract In many diseases such as interstitial lung (ILDs), patient diagnosis relies on diagnostic analysis of bronchoalveolar lavage fluid (BALF) and biopsies. BALF the differentiation neutrophils, eosinophils, lymphocytes, macrophages can contribute to diagnose underlying ILD entity. To analyze standard cytological techniques are labor-intensive time-consuming. Studies have shown promising cell identification performance blood fractions analyzed by third harmonic generation (THG)...
Abstract During thyroid surgery fast and reliable intra-operative pathological feedback has the potential to avoid a two-stage procedure significantly reduce health care costs in patients undergoing diagnostic hemithyroidectomy (HT). However, with current techniques, assessment of histopathological characteristics is not always within operation time. Therefore, there an urgent need for techniques that provide surgeon better during operation. As step towards this purpose, higher harmonic...
During thyroid surgery fast and reliable intra-operative pathological feedback has the potential to avoid a two-stage procedure significantly reduce health care costs in patients undergoing diagnostic hemithyroidectomy (HT). We explored higher harmonic generation (HHG) microscopy, which combines second (SHG), third (THG), multiphoton excited autofluorescence (MPEF) for this purpose. With compact, portable HHG microscope, images of freshly excised healthy tissue, benign nodules (follicular...
Third and second harmonic generation (THG/SHG) microscopy is a novel imaging technique that has been suggested as promising clinical tool, mainly for cancer diagnosis.Here, we test the first time compact, transportable ex-vivo THG/SHG microscope (Tritos Diagnostics B.V.) use.In lung surgery, immediate feedback on nature of excised tissue important.We will present results an ongoing study in which image both tumor immediately after excision frozen section biopsies, compare images to golden...
<b>Rationale:</b> Cryobiopsy is increasingly used for patients with interstitial lung disease (ILD) when biopsy indicated. However, conceivably due to sampling error, diagnostic yield 15% less than surgical biopsy. Higher harmonic generation (HHG) microscopy a laser imaging technique scan specimens resulting in submicron resolution images without need processing or staining tissue. Hereby HHG allow instant identification of tissue morphology and cellular structures. <b>Methods:</b> ILD an...
The inherited bone disease Osteogenesis Imperfecta (OI) caused by genetic mutation can lead to brittle bones that fracture easily and serious skeletal deformities due collagen defects. To understand this disease, in vitro models of 2D monolayer fibroblast cultures obtained from subdermal skin biopsies were developed worldwide research groups, nowadays extended 3D further mimic disease. allow for studying the formation possible abnormalities extracellular matrix (ECM) will enable studies...
<b>Introduction:</b> Treatment for lung cancer patients requires a tissue diagnosis. To date, the absence of on-site feedback on quality histology samples regularly results in non-diagnostic and repeat procedures. Higher harmonic generation microscopy (HHGM) provides rapid histological images unprocessed tissue. <b>Aim:</b> Assess feasibility HHGM instant bronchoscopic pleural biopsy pathological assessment. <b>Methods:</b> Fresh biopsies undergoing diagnostic bronchoscopy or thoracoscopy...
<b>Introduction:</b> For ILD patients, TBCB is the suggested initial technique when lung biopsy indicated. However, its diagnostic yield less than surgical due to smaller and sometimes inadequate samples. Higher harmonic generation microscopy (HHGM) provides instant histological images of unprocessed tissue with potential asses sample adequacy. <b>Aim:</b> Feasibility HHGM assess <b>Methods:</b> Observational study in patients undergoing TBCB. Fresh samples were imaged mobile (Flash...
<b>Introduction:</b> Lung biopsy in ILD comes with considerable morbidity and includes the risk of sampling error. Higher harmonic generation (HHG) microscopy is a laser-based imaging technique, that provides real-time images tissue submicron resolution without need for processing or staining tissue. <b>Methods:</b> patients undergoing bronchoscopic lung cryobiopsy were included. Following extraction, unprocessed was imaged mobile HHG microscope. Subsequently, biopsies processed according to...
We show a portable third and second harmonic generation microscope in the clinic generating 3D real-time high resolution images of fresh lung tumor tissue providing immediate pathological feedback for clinicians potentially reducing endoscopy/operation time.