A5080767698- Nuclear Structure and Function
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Nanoparticle-Based Drug Delivery
- Tissue Engineering and Regenerative Medicine
- Phagocytosis and Immune Regulation
- Electrospun Nanofibers in Biomedical Applications
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- bioluminescence and chemiluminescence research
- Photoreceptor and optogenetics research
- Cancer, Stress, Anesthesia, and Immune Response
- Renal and related cancers
- Laser Applications in Dentistry and Medicine
- 3D Printing in Biomedical Research
- Bacteriophages and microbial interactions
- DNA and Nucleic Acid Chemistry
- Anesthesia and Neurotoxicity Research
- Plant and Biological Electrophysiology Studies
- Sperm and Testicular Function
- Neuropeptides and Animal Physiology
- Reproductive System and Pregnancy
- Cell death mechanisms and regulation
Technion – Israel Institute of Technology
2010-2025
University of California, Davis
2018-2019
Rappaport Family Institute for Research in the Medical Sciences
2013-2015
Overexpressed extracellular matrix (ECM) in pancreatic ductal adenocarcinoma (PDAC) limits drug penetration into the tumor and is associated with poor prognosis. Here, we demonstrate that a pretreatment based on proteolytic-enzyme nanoparticle system disassembles dense PDAC collagen stroma increases tumor. More specifically, collagozome, 100 nm liposome encapsulating collagenase, was rationally designed to protect collagenase from premature deactivation prolonged its release rate at target...
Abstract Monoclonal antibodies (mAbs) hold promise in treating Parkinson's disease (PD), although poor delivery to the brain hinders their therapeutic application. In current study, it is demonstrated that brain‐targeted liposomes (BTL) enhance of mAbs across blood‐brain‐barrier (BBB) and into neurons, thereby allowing intracellular extracellular treatment PD brain. BTL are decorated with transferrin improve targeting through overexpressed transferrin‐receptors on BBB during PD. loaded...
Abstract Development of regulated cellular processes and signaling methods in synthetic cells is essential for their integration with living materials. Light an attractive tool to achieve this, but the limited penetration depth into tissue visible light restricts its usability in-vivo applications. Here, we describe design implementation bioluminescent intercellular intracellular mechanisms cells, dismissing need external source. First, engineer generating SCs optimized lipid membrane...
Progress in bottom-up synthetic biology has stimulated the development of cells (SCs), autonomous protein-manufacturing particles, as dynamic biomimetics for replacing diseased natural and addressing medical needs. Here, we report that SCs genetically encoded to produce proangiogenic factors triggered physiological process neovascularization mice. The were constructed giant lipid vesicles optimized facilitate enhanced protein production. When introduced with appropriate genetic code,...
The 5' untranslated region of the FMR1 gene which normally includes 4-55 d(CGG) repeats expands to > 55-200 in carriers fragile X syndrome premutation. Although levels premutation mRNA carrier cells are 5-10-fold higher than normal, amount product FMR protein is unchanged or reduced. We demonstrated previously that r(CGG)(n) tracts formed quadruplex structures impeded translation and lowered efficiency synthesis. Normal could be restored vivo by destabilizing action CBF-A hnRNP A2 proteins....
Throughout the female menstrual cycle, physiological changes occur that affect biodistribution of nanoparticles within reproductive system. We demonstrate a 2-fold increase in nanoparticle accumulation murine ovaries and uterus during ovulation, compared to nonovulatory stage, following intravenous administration. This pattern had positive or negative effects when drug-loaded nanoparticles, sized 100 nm smaller, were used treat different cancers. For example, treating ovarian cancer with...
Multiple myeloma (MM) poses a significant therapeutic challenge due to its persistent progression and low survival rate. Although the proteasome inhibitor bortezomib has revolutionized MM treatment, aggressiveness drug resistance remain critical concerns. To tackle this problem, we developed AMD3100-targeted Bortezomib Liposomes (ATBL) designed for targeted delivery of cells. Uptake ATBL into cells was dependent on CXCR4 enhanced compared nontargeted liposomes, both in vitro vivo. Treating...
Abstract Synthetic cells (SCs), developed through bottom‐up synthetic biology, hold great potential for biomedical applications, with the promise of replacing malfunctioning natural and treating diseases spatiotemporal control. Currently, most SC synthesis characterization processes are manual, limiting scalability efficiency. In this study, an automated method is large‐scale production protein‐producing SCs therapeutic applications. The optimized process, compatible a robotic liquid...
Targeting neurons in breast cancer with anesthetic nanoparticles inhibits nerve-cancer stimulation and tumor progression.
Gene promoters are enriched in guanine clusters that potentially fold into quadruplex structures.Such quadruplexes were implicated the regulation of gene expression, plausibly by interacting with transcription factors.We showed previously homodimers myogenic factor MyoD bound vitro most tightly bimolecular promoter sequences musclespecific genes.By contrast, MyoD-E47 heterodimers formed tighter complexes d(CANNTG) E-box motifs govern muscle expression.Here, we show DNA enhance vivo and...
Glial phagocytosis of superfluous neurons and damaged or aberrant neuronal material is crucial for normal development maintenance the CNS. However, molecular mechanisms underlying relationship between death glial are poorly understood. We describe a novel mechanism that able to synchronize cell dying in Drosophila embryonic This involves c-Jun N-terminal kinase (JNK) signaling, which required developmental apoptosis specific during embryogenesis. demonstrate dJNK pathway gain-of-function...
Males and females respond differently to medications due physiologic, metabolic, genetic factors. At times, sex‐related differences cannot be mitigated by dose adjustment body mass, are evident from the tissue level single cell. The rising number of clinically approved nanotechnologies calls for assessing how their activity is affected patient's sex. Herein, sex in nanotechnology scoped, with emphasis on molecular considerations. Sex‐specific pharmacokinetics nanocarriers influenced...
Four myogenic regulatory factors (MRFs); MyoD, Myf-5, MRF4 and Myogenin direct muscle tissue differentiation. Heterodimers of MRFs with E-proteins activate muscle-specific gene expression by binding to E-box motifs d(CANNTG) in their promoters or enhancers. We showed previously that contrast the favored MyoD-E47 heterodimers, homodimeric MyoD associated preferentially quadruplex structures sequences genes. To inquire whether other shared DNA preferences affinities hetero- homo-dimeric were...
New techniques able to monitor the maturation of tissue engineered constructs over time are needed for a more efficient control developmental parameters. Here, label‐free fluorescence lifetime imaging (FLIm) approach implemented through single fiber‐optic interface is reported nondestructive in situ assessment vascular biomaterials. Recellularization processes antigen removed bovine pericardium scaffolds with endothelial cells and mesenchymal stem were evaluated on serous fibrous sides...
Abstract Mechanisms regulating nuclear organization control fundamental cellular processes, including the cell and chromatin organization. Their disorganization, aberrant architecture, has been often implicated in transformation. Here, we identify Lamin A, among proteins essential for as SPANX (sperm protein associated with nucleus on X chromosome), a cancer testis antigen previously linked to invasive tumor phenotypes, interacting melanoma. interaction A was mapped immunoglobulin fold-like...
Abstract Grapevine leafroll disease (GLD) is a globally spreading viral infection that causes major economic losses by reducing crop yield, plant longevity, and berry quality, with no effective treatment. associated virus‐3 (GLRaV‐3) the most severe, prevalent GLD strain affecting wine production. Here, ability of RNA interference (RNAi), non‐GMO gene‐silencing pathway, to treat GLRaV‐3 in infected Cabernet Sauvignon grapevines evaluated. Lipid‐modified polyethylenimine (lmPEI) synthesized...