- Research in Cotton Cultivation
- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant Molecular Biology Research
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Genetic diversity and population structure
- Plant Ecology and Taxonomy Studies
- Plant Taxonomy and Phylogenetics
- Botany, Ecology, and Taxonomy Studies
- Genomics and Phylogenetic Studies
- Ecology and Vegetation Dynamics Studies
- Plant and Fungal Species Descriptions
- Chromosomal and Genetic Variations
- Rangeland and Wildlife Management
- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Botanical Research and Applications
- Bioenergy crop production and management
- Biofuel production and bioconversion
- Plant and animal studies
- CRISPR and Genetic Engineering
- solar cell performance optimization
- Plant and Biological Electrophysiology Studies
- Environmental DNA in Biodiversity Studies
Texas A&M University
2013-2023
Mitchell Institute
2011-2023
Academy of Sciences Republic of Uzbekistan
2007
United States Department of Agriculture
2007
Agricultural Research Service
2007
Salk Institute for Biological Studies
1993-1998
University of California, Davis
1987-1995
Summary PCR‐based detection of single nucleotide polymorphisms is a powerful tool for the plant geneticist. Cleaved amplified polymorphic sequence analysis most widely used approach polymorphisms. However, this technique limited to mutations which create or disrupt restriction enzyme recognition site. This paper presents modification where mismatches in PCR primer are polymorphism based on target mutation. useful following known segregating populations and genetic mapping isolated DNAs...
Abstract High-throughput genotyping arrays provide a standardized resource for plant breeding communities that are useful breadth of applications including high-density genetic mapping, genome-wide association studies (GWAS), genomic selection (GS), complex trait dissection, and studying patterns diversity among cultivars wild accessions. We have developed the CottonSNP63K, an Illumina Infinium array containing assays 45,104 putative intraspecific single nucleotide polymorphism (SNP) markers...
Genetic linkage maps play fundamental roles in understanding genome structure, explaining formation events during evolution, and discovering the genetic bases of important traits. A high-density cotton (Gossypium spp.) map was developed using representative sets simple sequence repeat (SSR) first public set single nucleotide polymorphism (SNP) markers to genotype 186 recombinant inbred lines (RILs) derived from an interspecific cross between Gossypium hirsutum L. (TM-1) G. barbadense (3-79)....
The Cotton Microsatellite Database (CMD) http://www.cottonssr.org is a curated and integrated web-based relational database providing centralized access to publicly available cotton microsatellites, an invaluable resource for basic applied research in breeding. At present CMD contains publication, sequence, primer, mapping homology data nine major microsatellite projects, collectively representing 5,484 microsatellites. In addition, displays three of the projects that have been screened...
In seeds and other parts of cultivated, tetraploid cotton (Gossypium hirsutum L.), multicellular groups cells lysigenously form dark glands containing toxic terpenoids such as gossypol that defend the plant against pests pathogens. Using RNA-seq analysis embryos from near-isogenic glanded (Gl2 Gl2 Gl3 ) versus glandless (gl2 gl2 gl3 plants, we identified 33 genes expressed exclusively or at higher levels in just prior to gland formation plants. Virus-induced gene silencing three pairs led...
Cotton germplasm resources contain beneficial alleles that can be exploited to develop adapted emerging environmental and climate conditions. Accessions lines have traditionally been characterized based on phenotypes, but phenotypic profiles are limited by the cost, time, space required make visual observations measurements. With advances in molecular genetic methods, genotypic increasingly able identify differences among accessions due larger number of markers measured. A combination both...
The genus Boechera is a widespread North American group with great potential for studies of ecology and evolution: closely related to Arabidopsis exhibits different ecological reproductive strategies. stricta (previously Arabis drummondii) morphologically genetically well-defined, perennial crucifer species. Fifteen natural populations diploid individuals from the Rocky Mountains were analysed using 21 microsatellite loci. In accordance our expectation this predominately inbreeding species,...
There are two enolase genes, ENO1 and ENO2, per haploid yeast genome. Expression of the gene is quantitatively similar in cells grown on glucose or gluconeogenic carbon sources. In contrast, ENO2 expression induced more than 20-fold as source. cis-Acting regulatory sequences were mapped within 5'-flanking region constitutively expressed ENO1. A complex positive was located 445 base pairs (bp) upstream from transcriptional initiation site which required for glycolytic negative 160 bp site....
Light regulation of seedling morphogenesis is mediated by photoreceptors that perceive red, far-red, blue and UV light. Photomorphogenetic mutants Arabidopsis have identified several the primary photoreceptors, as well a set negative regulators photomorphogenesis, including DET1, appear to act downstream photoreceptors. To study regulatory context in which DET1 acts repress we used simple morphological screen isolate extragenic mutations six loci, designated ted (for reversal det phenotype),...
Abstract To investigate the evolution of clinal variation in an invasive plant, we compared cold hardiness introduced saltcedar ( Tamarix ramosissima , chinensis and hybrids) native plains cottonwood Populus deltoides subsp. monilifera ). In a shadehouse Colorado (41°N), grew plants collected along latitudinal gradient central United States (29–48°N). On 17 occasions between September 2005 June 2006, determined killing temperatures using freeze‐induced electrolyte leakage direct observation....
Imperata cylindrica (Cogongrass, Speargrass) is a diploid C4 grass that noxious weed in 73 countries and constitutes significant threat to global biodiversity sustainable agriculture. We used cost-effective genotyping-by-sequencing (GBS) approach identify the reproductive system, genetic diversity geographic origins of invasions south-eastern United States. In this work, we demonstrated advantage employing closely related, fully sequenced crop species Sorghum bicolor (L.) Moench as proxy...
Karenia brevis is a harmful algal species that blooms in the Gulf of Mexico and produces brevetoxins cause neurotoxic shellfish poisoning. Elevated brevetoxin levels K. cells have been measured during laboratory hypo-osmotic stress treatments. To investigate mechanisms underlying osmoacclimation osmoregulation establish valuable resource for gene discovery, we assembled reference transcriptomes three clones: Wilson-CCFWC268, SP3, SP1 (a low-toxin producing variant). were annotated with...