A5026050012- Peripheral Neuropathies and Disorders
- Hereditary Neurological Disorders
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Cellular Mechanics and Interactions
- Cardiac Fibrosis and Remodeling
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Cardiovascular Function and Risk Factors
- Myasthenia Gravis and Thymoma
- Virology and Viral Diseases
- Cardiac Ischemia and Reperfusion
- Nerve injury and regeneration
- Animal Disease Management and Epidemiology
- Cell Adhesion Molecules Research
- Clusterin in disease pathology
- Immune Response and Inflammation
- Pluripotent Stem Cells Research
- Heme Oxygenase-1 and Carbon Monoxide
- Neuroinflammation and Neurodegeneration Mechanisms
- CRISPR and Genetic Engineering
- Neuroscience and Neural Engineering
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Cell Function and Interaction
- Hemoglobinopathies and Related Disorders
Boehringer Ingelheim (Austria)
2023-2025
Ludwig-Maximilians-Universität München
2012-2018
Technical University of Munich
2015-2017
Klinikum rechts der Isar
2016-2017
German Centre for Cardiovascular Research
2014-2015
Max Planck Institute of Neurobiology
2012-2015
Max Planck Institute of Biochemistry
2015
Munich Cluster for Systems Neurology
2015
Harvard University
2015
German Center for Neurodegenerative Diseases
2015
We asked whether autoantibodies against neurofascin (NF)186 or NF155, both localized at the nodes of Ranvier, are present in serum patients with inflammatory neuropathy, and NF-specific monoclonal antibodies pathogenic vivo.We cloned human NF155 NF186, developed an ELISA cell-based assay to screen for NF a total 434 donors including 294 Guillain-Barré syndrome variants acute demyelinating polyneuropathy (AIDP), motor axonal chronic (CIDP). characterized reactive samples by isotyping, tissue...
Disrupting the HNP1 and CCL5 heteromer between neutrophils platelets blocks monocyte recruitment to inflammatory sites.
Cardiomyocytes (CMs) generated from human induced pluripotent stem cells (hiPSCs) are increasingly used in disease modelling and drug evaluation. However, they typically a heterogeneous mix of ventricular-, atrial-, nodal-like based on action potentials (APs) gene expression. This heterogeneity the paucity methods for high-throughput functional phenotyping hinder full exploitation their potential. We aimed at developing method rapid, sequential, subtype-specific hiPSC-CMs with respect to AP...
To characterize phenotypes of T cells that accumulated in multiple sclerosis (MS) lesions, to compare the lesional T-cell receptor (TCR) repertoire subsets peripheral blood, and identify paired α β chains from single CD8(+) an index patient who we followed for 18 years.We combined immunohistochemistry, laser microdissection, single-cell multiplex PCR subtypes TCRα TCRβ individual brain-infiltrating frozen brain sections. The TCR repertoires were analyzed by pyrosequencing.We found a Vβ1(+)...
Recently, IgG autoantibodies against different paranodal proteins have been detected and this has led to important advances in the management of inflammatory neuropathies. In contrast, not much is known on IgM proteins.In present study, we screened a large cohort patients (n=140) with neuropathies for neurofascin-155, neurofascin-186 or contactin-1.IgM neurofascin-155 were by ELISA five patients, four demyelinating polyradiculoneuropathy (CIDP) one Guillain-Barré syndrome (GBS), confirmed...
Multifocal motor neuropathy is an immune mediated disease presenting with multifocal muscle weakness and conduction block. IgM auto-antibodies against the ganglioside GM1 are detectable in about 50% of patients. Auto-antibodies paranodal proteins contactin-1 neurofascin-155 nodal protein neurofascin-186 have been detected subgroups patients chronic inflammatory demyelinating polyneuropathy. Recently, gliomedin were described more than 60% neuropathy. In current study, we aimed to validate...
Objective: The actin polymerization regulator Thymosin β4 (Tβ4) has been shown to be involved in angiogenesis, wound healing, cell survival and anti-inflammatory responses. We have previously that Tβ4 is capable of recruiting pericytes, thus stabilizing the endothelial barrier function. Here, we analyzed whether treatment with able reduce pericytes loss lipopolysaccharides (LPS)-induced sepsis improve hemodynamic function C57BL/6 mice.Methods: Fourteen days before LPS injection, mice were...
Summary Advanced glycation end-products (AGEs) interact with their receptor RAGE, leading to an inflammatory state. We investigated the role of RAGE in postischaemic leukocyte adhesion after myocardial infarction and its effect on function. Wildtype (WT), ICAM-1-/-, RAGE-/- or ICAM-1/RAGE-/- mice underwent 20 minutes (min) LAD-occlusion followed by 15 min reperfusion. applied vivo fluorescence microscopy visualising Rhodamine-6G labelled leukocytes. To differentiate between endothelial we...
Virus particle (VP) quantification plays a pivotal role in the development of production processes VPs for virus-based therapies. The yield based on total VP count serves as process performance indicator evaluating efficiency and consistency. Here, label-free method enveloped was developed, with potential applications oncolytic virotherapy, vaccine development, gene therapy. comprises size-exclusion chromatography (SEC) separation using high-performance liquid (HPLC) instruments. Ultraviolet...
Abstract A new β°‐ halassemia mutation, a frameshift mutation with an insertion of single cytosine nucleotide in codon 27–28, is described. The propositus, who compound heterozygous for this and the IVSII‐654 C→T β°‐thalassemia has phenotype severe β‐thalassemia major.