Abstract The present study describes the distribution and morphological characteristics of neurons nerve fibers containing catecholamine‐synthesizing enzymes, tyrosine hydroxylase dopamine‐β‐hydroxylase, in sheep brainstem diencephalon on basis immunohistochemical procedures. Neurons were considered to be dopaminergic if they showed antityrosine immunoreactivity, without corresponding anti‐dopamine‐β‐hydroxylase immunoreactivity. structures labeled with both antisera noradrenergic or...

In anoestrous ewes, male chemosignals elicit rapid increases in luteinizing hormone (LH) secretion that can ultimately lead to ovulation. To assess the possible involvement of accessory (vomeronasal) olfactory system mediation those chemical cues, we destroyed this pathway by vomeronasal organ electrocauterization (Exp. I) and nerve section II). Neither these lesions inhibited LH response ewes odour male. These results suggest is not necessary mediate neuroendocrine ewe odour. As both...

Separate studies with ewes have shown that catecholamines play an inhibitory role in the control of LH secretion during anestrus, and there are structures lateral retrochiasmatic area (L-RCh), which could be involved regulation gonadotrophin secretion. These observations led to hypothesis catecholaminergic L-RCh mediate inhibition pulsatile by estradiol anestrous ewe. We tested this injecting 6-hydroxydopamine (60H-DA) into ovariectomized season, comparing prolactin these animals sham...

Objective: To compare the efficacy and tolerability of a new ionic silver alginate matrix (Askina Calgitrol Ag) with that standard silver-free dressing (Algosteril). Method: Patients locally infected chronic wounds (pressure ulcers, venous or mixed aetiology leg diabetic foot ulcers) acute were eligible for this prospective, open-label, controlled randomised trial. to receive one two dressings two-week period. Criteria based on evolution, from day 1 15, local signs infection using clinical...

The preoptic area contains most of the luteinizing hormone releasing immunoreactive neurons and numerous monoaminergic afferents whose cell origins are unknown in sheep. Using tract tracing methods with a specific retrograde fluorescent tracer, fluorogold, we examined cells origin to medial Among retrogradely labeled neurons, immunohistochemistry for tyrosine hydroxylase, dopamine-beta-hydroxylase, phenylethanolamine N-methyltransferase, serotonin was used characterize catecholamine...

As in other species, exogenous administration of ghrelin, an endogenous ligand for the growth hormone (GH) secretagogue receptors can stimulates feeding behaviour and GH secretion sheep. However, importance ghrelin these two functions as well its central or peripheral origin remained to be established. In present study, cerebrospinal fluid (CSF) concentrations were measured five anoestrous ewes found more than 1000-fold lower circulating plasma levels, keeping with even concentration...

Abstract During development, GnRH‐1 neurons differentiate extracerebraly from the nasal placode and migrate vomeronasal organ to forebrain along terminal nerves. Numerous studies have described influence of different molecules on migration neurons, however, role microenvironment cells remains poorly understood. This study used GFAP‐GFP transgenic mice detect glial at early developmental stages. Using explant cultures, comigration with was clearly demonstrated. in vitro approach showed that...

Invariant natural killer T (iNKT) cells represent a particular subset of lymphocytes capable producing several cytokines, which exert regulatory or effector functions, following stimulation the cell receptor. In this study, we investigated influence iNKT on development experimental anti-glomerular basement membrane glomerulonephritis (anti-GBM GN). After injection anti-GBM serum, number kidney rapidly increased. cell-deficient mice (Jα18−/−) injected with serum demonstrated worse renal...

Kisspeptins, encoded by the Kiss1 gene, play a key role in regulation of reproductive function, although very little is known about ontogenesis this system. The present study aimed to determine period arcuate nucleus (ARC) kisspeptin cell birth and embryonic stage neuroanatomical sites onset immunoreactivity. Bromodeoxyuridine (BrdU) was administered female rats at various gestational stages double immunohistochemistry against BrdU performed on brain sections from their offspring....

Postnatal treatment with bacterial endotoxin lipopolysaccharide (LPS) changes the activity of hypothalamic-pituitary-gonadal (HPG) axis and gonadotropin-releasing hormone (GnRH) surge in rats. Exposure to an immune challenge critical periods development has profound long-lasting effects on stress response, immune, metabolic, reproductive functions. Prenatal LPS delays migration GnRH neurons associated increased cytokine release maternal fetal compartments. We investigated a single exposure...

Estrogen exerts important feedback effects upon the biosynthetic and secretory behavior of gonadotropin-releasing hormone (GnRH) neurons to control reproductive functioning. The mechanism estrogen action these is unclear seems likely involve transsynaptic regulation GnRH neurons. objective present study was identify estrogen-receptive neural populations which project general vicinity perikarya in rostral preoptic area diagonal band Broca (rPOA/DBB) ewe. Intact breeding-season ewes received...

Several neurotransmitters are implicated in the photoperiodic regulation of prolactin and luteinising hormone (LH) secretion ewe. This work investigated whether catecholamines, γ ‐amino butyric acid (GABA), excitatory amino acids serotonin diencephalic contents affected by photoperiod how such changes relate to seasonal effects on LH secretions. Moreover, determine can influence catecholamine biosynthesis, activity its rate limiting enzyme, tyrosine hydroxylase (TH) was also investigated. TH...

Abstract The hypothalamus plays an important role in the control of food intake different species, but there is little relevant information for ruminants like sheep. In order to study putative several hypothalamic nuclei sheep, Fos expression, a marker cellular activity, was compared by immunohistochemistry between fed and unfed ewes. expression protein stimulated supraoptic nucleus ewes, whereas it increased paraventricular animals. latter nucleus, immunoreactivity mainly localized close...

Characterization of secretogranin II (SgII) mRNA in various vertebrates has revealed selective conservation the amino acid sequences two regions protein, i.e., bioactive peptide secretoneurin and a flanking novel that we named EM66. To help elucidate possible role EM66, examined occurrence as well cellular subcellular distribution EM66 rat pituitary adrenal glands by using polyclonal antibody raised against recombinant human peptide. High-performance liquid chromatography (HPLC) analysis...