A5079428350- Cellular transport and secretion
- Neuropeptides and Animal Physiology
- Hypothalamic control of reproductive hormones
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Adrenal and Paraganglionic Tumors
- Hormonal Regulation and Hypertension
- Cancer, Hypoxia, and Metabolism
- Reproductive biology and impacts on aquatic species
- Regulation of Appetite and Obesity
- Neurobiology and Insect Physiology Research
- Pancreatic function and diabetes
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Neuroendocrine regulation and behavior
- Selenium in Biological Systems
- Retinal Development and Disorders
- Protein Hydrolysis and Bioactive Peptides
- Pituitary Gland Disorders and Treatments
- Vitamin D Research Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Endoplasmic Reticulum Stress and Disease
- Metabolism, Diabetes, and Cancer
- Mitochondrial Function and Pathology
- Aquaculture Nutrition and Growth
- Apelin-related biomedical research
Différenciation et Communication Neuronale et Neuroendocrine
2008-2021
Neuroendocrine, Endocrine and Germinal Differentiation Communication
2020-2021
Université de Rouen Normandie
2007-2020
Inserm
2007-2020
Normandie Université
2003-2020
Institute for Research and Innovation in Biomedicine
2013-2020
Germinal (United Kingdom)
2020
Instituto Maimónides de Investigación Biomédica de Córdoba
2013
University of Córdoba
2013
Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition
2013
Selenoproteins contain the essential trace element selenium, deficiency of which is associated with cancer or accelerated aging. Although selenoproteins are thought to be instrumental for effects biological function many these proteins remains unknown. Here, we studied role selenoprotein T (SelT), a selenocysteine (Sec) -containing protein no known function, have identified as novel target gene neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) during PC12 cell...
The effect of steroids on the two gonadotropin-releasing hormone (GnRH) forms present in eel (mammalian GnRH, mGnRH and chicken GnRH-II, cGnRH-II), as well gonadotropin (GTH), was studied using specific radioimmunoassays. Female silver eels received chronic treatments with various (estradiol, testosterone, androstenedione, 5 alpha-androstane-3 beta, 17 beta-diol). Estradiol or combination estradiol androgens induced increases brain pituitary levels GTH level, whereas given alone had no...
The complementary DNA encoding pituitary adenylate cyclase-activating polypeptide (PACAP) has been cloned from two species of teleost fishes, the Sockeye salmon and Thai catfish, amino acid sequence PACAP determined in another teleost, stargazer. However, to date, detailed distribution immunoreactivity never investigated fish brain. In present study, we have localization PACAP-immunoreactive neurons central nervous system a primitive fish, European eel Anguilla anguilla, using an antiserum...
Chromogranin A (CgA) has been proposed to play a major role in the formation of dense-core secretory granules (DCGs) neuroendocrine cells. Here, we took advantage unique features frog CgA (fCgA) assess this granin and its potential functional determinants hormone sorting during DCG biogenesis. Expression fCgA constitutively secreting COS-7 cells induced mobile vesicular structures, which contained cotransfected peptide hormones. The hormones coexpressed newly formed vesicles could be...
A number of incidentally discovered pheochromocytomas are not associated with hypertension. The characteristics normotensive (NIPs) poorly known.The purpose this work was to assess the clinical, hormonal, histological, and molecular features NIPs.This a retrospective cohort recruited from 2001 2011 in 2 tertiary care medical departments.Clinical, biological, radiological investigations performed 96 consecutive patients sporadic unilateral were examined; 47 had overt responsible for Among...
Glycerophospholipids (GPLs) play important roles in cellular compartmentalization and signaling. Among them, phosphatidic acids (PA) exist as many distinct species depending on acyl chain composition, each one potentially displaying unique signaling function. Although the functions of PA have already been demonstrated multiple processes, specific individual remain obscure due to a lack appropriate tools. Indeed, current synthetic analogues fail preserve all natural PA. To circumvent these...
Processes underlying the formation of dense core secretory granules (DCGs) neuroendocrine cells are poorly understood. Here, we present evidence that DCG biogenesis is dependent on protein secretogranin (Sg) II, a member granin family pro-hormone cargo DCGs in cells. Depletion SgII expression PC12 leads to decrease both number and size impairs trafficking other regulated hormones. Expression fusion proteins secretory-deficient variant rescues pathway. SgII-containing vesicles share...
Specific forms of fatty acids are well known to have beneficial health effects, but their precise mechanism action remains elusive. Phosphatidic acid (PA) produced by phospholipase D1 (PLD1) regulates the sequential stages underlying secretory granule exocytosis in neuroendocrine chromaffin cells, as revealed pharmacological approaches and genetic mouse models. Lipidomic analysis shows that plasma membranes display distinct specific composition PA. Secretagogue-evoked stimulation triggers...
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Urotensin II (UII) and UII-related peptide (URP) are paralog neuropeptides whose existence distribution in mouse have not yet been investigated. In this study, we showed by HPLC/RIA analysis that the UII-immunoreactive molecule brain corresponds to a new UII(17) isoform. Moreover, calcium mobilization assays indicated URP were equally potent stimulating UII receptor (UT receptor). Quantitative RT-PCR situ hybridization revealed CNS mRNAs predominantly expressed brainstem spinal motoneurons....
Chromogranins are a family of acidic glycoproteins that play an active role in hormone and neuropeptide secretion through their crucial secretory granule biogenesis neuroendocrine cells. However, the molecular mechanisms underlying granulogenic activity still not fully understood. Because we previously demonstrated expression major component granules, chromogranin A (CgA), is able to induce formation granules nonendocrine COS-7 cells, decided use this model dissect triggered by CgA leading...
Chromogranin A (CgA) is a key luminal actor of secretory granule biogenesis at the trans-Golgi network (TGN) level but molecular mechanisms involved remain obscure. Here, we investigated possibility that CgA acts synergistically with specific membrane lipids to trigger formation. We show preferentially interacts anionic glycerophospholipid phosphatidic acid (PA). In accordance, bioinformatic analysis predicted PA-binding domain (PABD) in sequence effectively bound PA (36:1) or (40:6) models....
Characterization of secretogranin II (SgII) mRNA in various vertebrates has revealed selective conservation the amino acid sequences two regions protein, i.e., bioactive peptide secretoneurin and a flanking novel that we named EM66. To help elucidate possible role EM66, examined occurrence as well cellular subcellular distribution EM66 rat pituitary adrenal glands by using polyclonal antibody raised against recombinant human peptide. High-performance liquid chromatography (HPLC) analysis...
Abstract We have previously demonstrated that measurement of tissue concentration the novel secretogranin II‐derived peptide EM66 may help to discriminate between benign and malignant pheochromocytomas. The aim present study was characterize in plasma urine healthy volunteers pheochromocytoma patients, order further evaluate usefulness this as a circulating marker for management tumors. HPLC analysis samples EM66‐immunoreactive material coeluted with recombinant peptide. In volunteers,...
Hormone secretion relies on secretory granules which store hormones in endocrine cells and release them upon cell stimulation. The molecular events leading to hormone sorting granule formation at the level of TGN are still elusive. Our proteomic analysis purified whole or membranes uncovered their association with actomyosin components myosin 1b, actin nucleation complex Arp2/3. We found that 1b controls associated regulated both neuroendocrine chromogranin A-expressing COS7 used as a...
Abstract It is well recognized that the world population ageing rapidly. Therefore, it important to understand processes at cellular and molecular levels predict onset of age‐related diseases prevent them. Recent research has focused on identification biomarkers, including those associated with properties Golgi apparatus. In this context, Golgi‐mediated glycosylation proteins been characterized. Additionally, other studies show secretion many compounds, pro‐inflammatory cytokines...