A5042399385- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Adrenal and Paraganglionic Tumors
- Cancer, Hypoxia, and Metabolism
- Cellular transport and secretion
- Hemoglobinopathies and Related Disorders
- Hormonal Regulation and Hypertension
- Pancreatic function and diabetes
- Regulation of Appetite and Obesity
- Birth, Development, and Health
- Iron Metabolism and Disorders
- Mitochondrial Function and Pathology
- Neurobiology and Insect Physiology Research
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Pituitary Gland Disorders and Treatments
- Legionella and Acanthamoeba research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer, Lipids, and Metabolism
- Lipoproteins and Cardiovascular Health
- Blood Coagulation and Thrombosis Mechanisms
- Neuroblastoma Research and Treatments
- Hypothalamic control of reproductive hormones
- Diet and metabolism studies
- Trace Elements in Health
Centre International de Recherche en Infectiologie
2020-2023
École Normale Supérieure de Lyon
2022-2023
Université Claude Bernard Lyon 1
2020-2023
Inserm
2003-2023
Centre National de la Recherche Scientifique
2003-2023
Institut des Maladies Métaboliques et Cardiovasculaires
2022
Université Jean Monnet
2022
Différenciation et Communication Neuronale et Neuroendocrine
2003-2019
Normandie Université
2019
Université de Rouen Normandie
2003-2019
Epidemiological studies suggest that maternal undernutrition predisposes the offspring to development of energy balance metabolic pathologies in adulthood. Using a model prenatal 70% food-restricted diet (FR30) rats, we evaluated peripheral parameters involved nutritional regulation, as well hypothalamic appetite-regulatory system, nonfasted and 48-h-fasted adult offspring. Despite comparable glycemia both groups, mild glucose intolerance, with defect glucose-induced insulin secretion, was...
Significance The 7-membered proprotein convertase (PC) family of basic amino acid-specific proteases is implicated in the cleavage-activation secretory precursor proteins. Although vivo functions most members have been well studied, those seventh member PC7 are largely unknown. Herein, we find that participates generation mature BDNF both cells and specific brain areas such as hippocampus amygdala. Indeed, exerts a critical role brain: results various behavioral tests normal mice compared...
Abstract The first seven members of the proprotein convertase (PC) family activate protein precursors by cleavage after basic residues. While PC7 has no known specific substrates, it shows redundancy with other PCs. A genome-wide association study suggested that circulating levels shed human transferrin receptor 1 (hTfR1) are regulated PC7. We thus examined whether hTfR1 constitutes a substrate for Coexpression PCs in several cell lines indicated is only sheds this into medium. Site-directed...
Amyloid precursor-like protein 2 (APLP2) and sortilin were reported to individually bind the proprotein convertase subtilisin/kexin type 9 (PCSK9) regulate its activity on low-density lipoprotein receptor (LDLR). The data presented herein demonstrate that mRNA knockdowns of APLP2, sortilin, or both in human hepatocyte cell lines HepG2 Huh7 do not affect ability extracellular PCSK9 enhance degradation LDLR. Furthermore, mice deficient APLP2 exhibit significant changes liver LDLR plasma total...
Pheochromocytomas are catecholamine-producing tumors that generally benign but can also present as or develop into malignancy. Occurrence of malignant pheochromocytomas only be asserted by imaging metastatic lesions.We conducted a gene expression profiling and to identify signature would allow us discriminate from gain better understanding tumorigenic pathways associated with malignancy.A total 36 patients pheochromocytoma was studied retrospectively. There were 18 (nine nine malignant) used...
Insulin-like growth factor 2 (IGF2) overexpression is an important molecular marker of adrenocortical carcinoma (ACC), which a rare but devastating endocrine cancer. It not clear whether IGF2 modifies the biology and this cancer, thus more studies are required before can be considered as major therapeutic target. We compared phenotypical, clinical, biological, characteristics ACC with or without IGF2, to address these issues. also carried out similar analysis in cell line (H295R) expression...
établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics privés.
Recently, secondary organic aerosols (SOAs) emerged as a predominant component of fine particulate matter. However, the pathogenic mechanism(s) SOAs are still poorly understood. Herein, we show that chronic exposure mice to resulted in lung inflammation and tissue destruction. Histological analyses found airspace enlargement associated with massive inflammatory cell recruitment predominated by macrophages. Concomitant such influx, our results changes levels series mediators response SOA....
The gastroprokinetic agent metoclopramide is known to stimulate catecholamine secretion from pheochromocytomas. aim of the study was investigate mechanism action and expression serotonin type 4 (5-HT(4)) receptors in pheochromocytoma tissues. Tissue explants, obtained 18 pheochromocytomas including tumor removed a 46-year-old female patient who experienced life-threatening hypertension crisis after administration 17 additional (9 benign 8 malignant) were studied. Cultured cells derived...
Abstract Transcriptional cofactors YAP/TAZ have recently been found to support autophagy and inflammation, which are part of cell-autonomous immunity critical in antibacterial defense. Here, we studied the role YAP against Staphylococcus aureus using CRISPR/Cas9-mutated HEK293 cells a primary cell-based organoid model. We that S. infection increases transcriptional activity, is required reduce intracellular replication. A 770-gene targeted transcriptomic analysis revealed upregulates genes...
Characterization of secretogranin II (SgII) mRNA in various vertebrates has revealed selective conservation the amino acid sequences two regions protein, i.e., bioactive peptide secretoneurin and a flanking novel that we named EM66. To help elucidate possible role EM66, examined occurrence as well cellular subcellular distribution EM66 rat pituitary adrenal glands by using polyclonal antibody raised against recombinant human peptide. High-performance liquid chromatography (HPLC) analysis...
Legionnaires' Disease (LD) is a severe pneumonia mainly caused in Europe by Legionella pneumophila serogroup 1 (Lp1). Sequence-based typing methods reveal that some sequence types (ST) are overrepresented clinical samples such as ST1 and ST47, suggesting strains more fit for infection than others. In the present study, collection of 108 Lp1 isolates were used to evaluate strain-dependent immune responses from human macrophages. Clinical induced differential TNFα secretion significantly...
Abstract We have previously demonstrated that measurement of tissue concentration the novel secretogranin II‐derived peptide EM66 may help to discriminate between benign and malignant pheochromocytomas. The aim present study was characterize in plasma urine healthy volunteers pheochromocytoma patients, order further evaluate usefulness this as a circulating marker for management tumors. HPLC analysis samples EM66‐immunoreactive material coeluted with recombinant peptide. In volunteers,...
Pheochromocytomas are catecholamine-producing tumors which generally benign, but can also present as or develop into malignancy. Molecular pathways of malignant transformation remain poorly understood. express various trophic peptides may influence tumoral cell behavior. Here, we investigated the expression amidated peptides, including pituitary adenylate cyclase-activating polypeptide (PACAP), neuropeptide Y (NPY), and adrenomedullin (AM), their receptors in benign pheochromocytomas order...
Abstract: Pheochromocytomas (PHEOs) are rare catecholamine‐producing neoplasias that arise from chromaffin cells of the adrenal medulla or extra‐adrenal locations. These neuroendocrine tumors usually benign, but may also present as develop into a malignancy. There currently no means to predict cure malignant which have poor prognosis. We recently validated several assays for measurement peptides derived chromogranin A (CgA) and secretogranin II (SgII) in order determine whether these...
Granins and their derived peptides are valuable circulating biological markers of neuroendocrine tumors. The aim the present study was to investigate tumoral chromogranin A (CgA)-derived peptide WE-14 potential advantage combine plasma detection with EM66 assay existing current CgA for diagnosis pheochromocytoma. Compared healthy volunteers, levels were 5.4-fold higher in patients pheochromocytoma, but returned normal values after surgical resection tumor. Determination concentrations same...
Scope Iron overload contributes to the pathogenesis of atherosclerosis and iron chelators are beneficial through their antioxidant properties. Hepatic loading increases cholesterol synthesis. Whether depletion could affect hepatic metabolism is unknown. Methods results We examined effect chelator deferoxamine (DFO) on mRNA expression genes involved in and/or uptake. Our revealed that DFO LDL receptor (LDLR) levels human hepatocyte‐derived cell lines HepG2 Huh7 cells, K562 cells. In we...
Abstract: The aim of the present study was to compare expression levels secretogranin II (SgII), prohormone convertases (PC)1 and PC2, proteolytic processing SgII in benign versus malignant pheochromocytomas. Quantitative (Q)‐PCR experiments indicated that SgII, PC1, PC2 mRNAs were overexpressed pheochromocytoma compared non‐tumoral chromaffin cells ( P < 0.001) tumors 0.01). Western blot analysis using a human antiserum revealed occurrence 97‐kDa band corresponding expected size with...
The neuroendocrine protein secretogranin II is the precursor of several neuropeptides, including secretoneurin and a novel 66-amino acid peptide, EM66, sequence which has been highly conserved across vertebrae phylum. presence EM66 detected in adult fetal human adrenal gland, as well rat pituitary glands. present study aimed to explore possible role by analysing its occurrence distribution within jerboa hypothalamus, potential implication control feeding behaviour. High-performance liquid...
Introduction Severe Legionnaires’ disease (LD) can lead to multi-organ failure or death in 10%–30% of patients. Although hyper-inflammation and immunoparalysis are well described sepsis associated with high severity, little is known about the immune response LD. This study aimed evaluate status patients LD its association severity. Methods A total 92 hospitalized were included; 19 plasmatic cytokines pulmonary Legionella DNA load measured 84 on day inclusion (day 0, D0). Immune functional...
We have previously demonstrated that measurement of tissue concentrations the secretogranin II (SgII or SCG2 as listed in HUGO database)-derived peptide EM66 may help to discriminate between benign and malignant pheochromocytomas represents a sensitive plasma marker pheochromocytomas. Here, we investigated gene expression protein production SgII 13 normal adrenal glands, 35 16 with goal examine molecular mechanisms leading marked variations tumoral chromaffin tissue. levels were 16-fold...