A5024019018- Legionella and Acanthamoeba research
- Immune Response and Inflammation
- Sepsis Diagnosis and Treatment
- Reproductive tract infections research
- Urinary Tract Infections Management
- Adrenal Hormones and Disorders
- Immune Cell Function and Interaction
- Antifungal resistance and susceptibility
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- HIV, Drug Use, Sexual Risk
- Bacterial Identification and Susceptibility Testing
- Pneumocystis jirovecii pneumonia detection and treatment
- Infectious Encephalopathies and Encephalitis
- Actinomycetales infections and treatment
- Streptococcal Infections and Treatments
- Nosocomial Infections in ICU
- Syphilis Diagnosis and Treatment
- Antibiotic Resistance in Bacteria
- Long-Term Effects of COVID-19
- Pneumonia and Respiratory Infections
- Antimicrobial Resistance in Staphylococcus
- Infective Endocarditis Diagnosis and Management
- Heme Oxygenase-1 and Carbon Monoxide
Hospices Civils de Lyon
2016-2024
Centre International de Recherche en Infectiologie
2019-2024
Université Claude Bernard Lyon 1
2017-2024
Hôpital de la Croix-Rousse
2023-2024
Centre National de la Recherche Scientifique
2022-2024
Inserm
2019-2024
École Normale Supérieure de Lyon
2022-2024
bioMérieux (France)
2017-2019
Hôpital Edouard Herriot
2017-2019
Abstract Background Legionnaires’ disease (LD) is a common but under-diagnosed cause of community-acquired pneumonia (CAP), although rapid detection urine antigen testing (UAT) and advances in molecular have improved the diagnosis. LD entails intensive care unit (ICU) admission almost one-third cases, mortality rate ranges from 4% to 40%. This review aims discuss recent study this condition provide an update on diagnosis, pathogenesis management severe LD. Results The overall incidence has...
Disk diffusion testing is widely used to detect methicillin resistance in staphylococci, and cefoxitin currently considered the best marker for mecA-mediated resistance. In low-inoculum (colony suspension at 106 CFU/ml), addition of moxalactam combination with has been reported improve on alone detection methicillin-heteroresistant staphylococci. However, absent from EUCAST CLSI guidelines, which use high-inoculum 108 calling into question potential interest including their recommendations....
Legionnaires' Disease (LD) is a severe pneumonia mainly caused in Europe by Legionella pneumophila serogroup 1 (Lp1). Sequence-based typing methods reveal that some sequence types (ST) are overrepresented clinical samples such as ST1 and ST47, suggesting strains more fit for infection than others. In the present study, collection of 108 Lp1 isolates were used to evaluate strain-dependent immune responses from human macrophages. Clinical induced differential TNFα secretion significantly...
Abstract Objectives Implementation of an antibiotic resistance detection tool in Legionella daily surveillance at the French National Reference Centre for Legionella. Methods Systematic WGS pneumophila isolates and bioinformatics specific mutations linked to resistance. Phenotypic validation detected by was performed broth microdilution method. Results More than 3000 L. strains were screened A macrolide resistance-associated A2052G mutation 23S rRNA gene identified genome eight from a hotel...
Sepsis is the leading cause of mortality for critically ill patients worldwide. Patients develop T lymphocyte dysfunctions to T-cell exhaustion associated with increased risk death. As interleukin-7 (IL-7) currently tested in clinical trials reverse these dysfunctions, it important evaluate expression its specific CD127 receptor on surface septic shock. Moreover, CD127lowPD-1high phenotype has been proposed as a marker chronic viral infections but never evaluated sepsis. The objective this...
Abstract We describe a March 2020 co-occurrence of Legionnaires' disease (LD) and coronavirus in France. Severe acute respiratory syndrome 2 co-infections were identified 7 49 patients from LD case notifications. Most elderly men with underlying conditions who had contracted severe pneumonia, illustrating the relevance co-infection screening.
Introduction Severe Legionnaires’ disease (LD) can lead to multi-organ failure or death in 10%–30% of patients. Although hyper-inflammation and immunoparalysis are well described sepsis associated with high severity, little is known about the immune response LD. This study aimed evaluate status patients LD its association severity. Methods A total 92 hospitalized were included; 19 plasmatic cytokines pulmonary Legionella DNA load measured 84 on day inclusion (day 0, D0). Immune functional...
Abstract Background International guidelines recommend the systematic screening for Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT) infections in all men who have sex with (MSM) engaged unprotected sex. However, optimal strategy remains unclear. We developed a modeling approach to optimize NG/CT MSM. Methods A compartmental model of infection was implemented. anal, pharyngeal, urine (APU) samples from MSM attending sexually transmitted clinic were used estimate rate, prevalence,...
ABSTRACT Nocardia recovery from pulmonary samples is challenging due to the lack of a specific medium and abundance overgrown respiratory flora. This study aimed compare amoeba plate test (APT), an amoebic coculture with Acanthamoeba castellanii , axenic culture recover samples. serial dilutions ( n = 15 strains seven species, concentrations ranging: 10 7 –10 2 CFU/mL) in water spiked sputa 8) were simultaneously plated on agar monolayer (APT) without (control). Culture positivity rates,...
Background: Legionnaires’ disease (LD) is a pneumonia with course that not easily predictable. This study aimed to evaluate the prognostic value of 3 Legionella pneumophila (Lp)-specific biomarkers in prospective multicentre cohort hospitalised LD patients.Methods: Lp pulmonary and serum DNA, as well lipopolysaccharide (LPS) were measured at baseline (D0) for 108 patients. The outcome criterion was mechanical ventilation (MV) status on D8. Sequential Organ Failure Assessment (SOFA) score,...
ABSTRACT Functional testing protocols are thought to be the gold standard for exploration of immune system. However, in terms routine analysis, they present numerous drawbacks and consequently their use is mainly limited research applications. In clinical context septic shock, characterized by marked lymphocyte alterations, a new approach intracellular cytokine measurement whole blood upon was evaluated proof-of-concept study. Following activation, simultaneous labeling Interferon-γ (IFN-γ),...