A5033001152- Sarcoidosis and Beryllium Toxicity Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Air Quality and Health Impacts
- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Air Quality Monitoring and Forecasting
- Plant Pathogens and Fungal Diseases
- Trace Elements in Health
- Cell Adhesion Molecules Research
- Genomics and Rare Diseases
- Essential Oils and Antimicrobial Activity
- Indoor Air Quality and Microbial Exposure
- Electron Spin Resonance Studies
- Inhalation and Respiratory Drug Delivery
- Protease and Inhibitor Mechanisms
- Protein Interaction Studies and Fluorescence Analysis
- Nanoparticle-Based Drug Delivery
- Bee Products Chemical Analysis
- Advanced biosensing and bioanalysis techniques
- Insect Pest Control Strategies
- Climate Change and Health Impacts
- Immune cells in cancer
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Mast cells and histamine
Université Claude Bernard Lyon 1
2015-2025
Hospices Civils de Lyon
2020-2023
Centre de Recherche en Cancérologie de Lyon
2023
Centre International de Recherche en Infectiologie
2016-2018
Centre National de la Recherche Scientifique
2015-2016
Inserm
2015-2016
Hôpital Lyon Sud
2016
Nanoparticles are nowadays largely investigated in the field of drug delivery. Among nanoparticles, protein-based particles paramount importance since they natural, biodegradable, biocompatible, and nontoxic. There several methods to prepare proteins containing but only a few studies have been dedicated preparation protein- based nanoparticles. Then, aim this work was report on bovine serum albumin (BSA)-based nanoparticles using well-defined nanoprecipitation process. Special attention has...
In the last few years, essential oils (EOs) derived from plants have aroused great interest due to their well-known antimicrobial activity. Unfortunately, they present several limitations in use, such as photosensitivity, temperature sensitivity, high volatility, and poor water solubility. The encapsulation technique represents a good solution these problems ensures protection of functional properties oils. this work, bergamot oil (BEO) sweet orange (OEO) loaded-Eudragit® RS 100 (EuRS100)...
In acutely injured lungs, massively recruited polymorphonuclear neutrophils (PMNs) secrete abnormally neutrophil elastase (NE). Active NE creates a localized proteolytic environment where various host molecules are degraded leading to impairment of tissue homeostasis. Among the hallmarks neutrophil-rich pathologies is disrupted epithelium characterized by loss cell-cell adhesion and integrity. Epithelial-cadherin (E-cad) represents one most important intercellular junction proteins. E-cad...
Histopathology is the gold standard for fungal infection (FI) diagnosis, but it does not provide a genus and/or species identification. The objective of present study was to develop targeted next-generation sequencing (NGS) on formalin-fixed tissue samples (FTs) achieve integrated histomolecular diagnosis. Nucleic acid extraction optimized first group 30 FTs with Aspergillus fumigatus or Mucorales by macrodissecting microscopically identified fungal-rich area and comparing Qiagen Promega...
Abstract Background In patients with chronic obstructive pulmonary disease (COPD), a sensitization to A. fumigatus has been related decline in lung function, but the role of fungal agents pathogenesis remains unclear. The main purpose present study was investigate whether cell inflammation could worsen after exposure spores vitro and then, mice, following cigarette smoke mimicking COPD. Methods inflammatory response alone or investigated culture models murine macrophages alveolar epithelial...
Sarcoidosis (OMIM 181000) is a multi-systemic granulomatous disorder of unknown origin. Despite multiple genome-wide association (GWAS) studies, no major pathogenic pathways have been identified to date. To find out relevant sarcoidosis predisposing genes, we searched for de novo and recessive mutations in 3 young probands with their healthy parents using whole-exome sequencing (WES) methodology. From the SARCFAM project based on national network collecting familial cases sarcoidosis,...
Recently, secondary organic aerosols (SOAs) emerged as a predominant component of fine particulate matter. However, the pathogenic mechanism(s) SOAs are still poorly understood. Herein, we show that chronic exposure mice to resulted in lung inflammation and tissue destruction. Histological analyses found airspace enlargement associated with massive inflammatory cell recruitment predominated by macrophages. Concomitant such influx, our results changes levels series mediators response SOA....
The airway exposure to Aspergillus fumigatus spores (AFsp) is associated with an inflammatory response, potentially leading allergic and/or chronic pulmonary aspergillosis. aim of our study better understand the host first in vitro, then vivo, following mice AFsp. We investigated response AFsp cell mono- and co-culture systems murine macrophages alveolar epithelial cells. were subjected two intranasal instillations using 105 Their lungs processed for histopathological analyses. In culture,...
Cryptococcus neoformans/gattii and Histoplasma capsulatum var. may present atypical histopathological features inducing diagnostic errors. We aimed to estimate the frequency of these in formalin-fixed tissue (FT) samples assess relevance an integrated histomolecular diagnosis using specific H. PCR panfungal followed by Sanger sequencing and/or targeted massive parallel (MPS). A total 27 FT from 23 patients with a cryptococcosis (n = 16 13 patients) or histoplasmosis 11 10 were...
Pulmonary inflammation usually involves strong neutrophil recruitment with a marked release of proteases such as elastase (NE). Noninvasive in vivo assessment unregulated activity the lungs would provide valuable diagnostic tool. Here, it is proposed to use Overhauser-enhanced magnetic resonance imaging (OMRI) mice where was induced by instillation lipopolysaccharide (LPS). OMRI contrast generated dedicated NE free radical substrate. The decayed more rapidly LPS-treated mouse than control...
Human neutrophil elastase is a proteolytic enzyme secreted by during inflammation and infection in lung environment.It has an important anti-bacterial activity through different pathways.However, it also action against lung's extracellular matrix which lead to disease related acute injuries.secretory leukocyte proteinase inhibitor (SLPI) naturally produced protein with inhibition main role.Different diseases were linked the imbalance of elastase/SLPI ratio environment.In addition, SLPI...
Secondary organic aerosols (SOAs) have emerged recently as a major component of fine particulate matter. Cell culture studies revealed role for SOAs in cell oxidative stress, toxicity and inflammation only few investigated short-term SOA exposure animal models. Here, mice were chronically exposed to naphthalene-derived one two months. Weight monitoring indicated marked mass loss, especially females, following chronic SOAs. Significantly, cytokine antibody microarray approach SOA-induced...