A5060653900- Advanced MRI Techniques and Applications
- Electron Spin Resonance Studies
- Advanced NMR Techniques and Applications
- Lanthanide and Transition Metal Complexes
- Cardiovascular Function and Risk Factors
- Mitochondrial Function and Pathology
- Cardiac Imaging and Diagnostics
- Atomic and Subatomic Physics Research
- Nanoparticle-Based Drug Delivery
- Cardiovascular and exercise physiology
- MRI in cancer diagnosis
- Muscle metabolism and nutrition
- Ultrasound Imaging and Elastography
- NMR spectroscopy and applications
- Adipose Tissue and Metabolism
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Antimicrobial Peptides and Activities
- Metabolism and Genetic Disorders
- Advanced Neuroimaging Techniques and Applications
- Ultrasonics and Acoustic Wave Propagation
- Lipid Membrane Structure and Behavior
- Liver Disease Diagnosis and Treatment
- Ultrasound and Hyperthermia Applications
- Nanoplatforms for cancer theranostics
Centre National de la Recherche Scientifique
2012-2024
Université de Bordeaux
2012-2024
Centre de Résonance Magnétique des Systèmes Biologiques
2012-2024
Galen University
2013-2016
Victor (Japan)
2013-2016
Aix-Marseille Université
2014-2015
Advanced Imaging Research (United States)
2011
Centre Hospitalier Universitaire de Bordeaux
1996-2001
Hôpital Xavier Arnozan
1996-2001
Hôpital Cardiologique du Haut-Lévêque
1998
Hydrophobically modified maghemite (γ-Fe2O3) nanoparticles were encapsulated within the membrane of poly(trimethylene carbonate)-b-poly(l-glutamic acid) (PTMC-b-PGA) block copolymer vesicles using a nanoprecipitation process. This formation method gives simple access to highly magnetic (MNPs) (loaded up 70 wt %) together with good control over size (100−400 nm). The simultaneous loading and doxorubicin was also achieved by nanoprecipitation. deformation vesicle under an applied field has...
With aging, most skeletal muscles undergo a progressive loss of mass and strength, process termed sarcopenia. Aging-related defects in mitochondrial energetics have been proposed to be causally involved However, changes muscle oxidative phosphorylation with aging remain highly controversial issue, creating pressing need for integrative approaches determine whether bioenergetics are impaired aged muscle. To address this was first investigated vivo the gastrocnemius adult (6 months) (21 male...
Multifunctional polymersomes loaded with maghemite nanoparticles and grafted an antibody, directed against human endothelial receptor 2, are developed as novel MRI contrast agents for bone metastasis imaging. Upon administration in mice bearing tumor grown from breast cancer cells, MR images show targeting enhanced retention of antibody-labeled at the site.
Lactate metabolism in the adult rat brain was investigated relation with concept of lactate trafficking between astrocytes and neurons. Wistar rats were infused intravenously a solution containing either [3‐ 13 C]lactate (534 m M ) or both glucose (750 ). The time courses concentration C enrichment blood determined. data indicated occurrence recycling through liver gluconeogenesis. yield labeling was, however, reduced when using glucose‐containing infusate. After 20‐min 1‐h infusion,...
In this study, we examined the effect of creatine ingestion on muscle power output, phosphocreatine resynthesis, inorganic phosphate and pH during repeated brief bouts maximal exercise. Nine healthy males performed plantar flexion before after (20 g ·day -1 for 6 days). The experimental protocol consisted five 8 s (bouts 1-5) interspersed with 30 recovery, followed by (8 s) 7 (16 separated 1 2 min, respectively. Muscle phosphocreatine, were estimated every 16 31 P magnetic resonance...
Staphylococcal δ‐toxin, a synthetic analogue and fragment were studied in order to determine their structure solution bound lipids. In solution, self‐association process is observed. Analytical ultracentrifuge quasi‐elastic light‐scattering experiments suggest an isodesmic aggregation the high concentration domain above 2 μM up very large asymmetrical species. Decreasing concentrations below of δ‐toxin allows dissociation, probably into monomers. The self‐associated species are essentially...
The improvement of commonly used Gd3+-based MRI agents requires the design new systems with optimized in vivo efficacy, pharmacokinetic properties, and specificity. To these contrast agents, two parameters are usually considered: increasing number coordinated water molecules or rotational correlation time by molecular weight size. This has been achieved noncovalent covalent binding low-molecular Gd3+ chelates to macromolecules polymers. grafting high-spin paramagnetic gadolinium on metal...
Proteolysis, involved in many processes living organisms, is tightly regulated space and time under physiological conditions. However deregulation can occur with local persistent proteolytic activities, e.g. inflammation, cystic fibrosis, tumors, or pancreatitis. Furthermore, little known about the role of proteases, hence there a need new imaging methods to visualize specifically normal disease-related proteolysis intact bodies.In this paper, concept for non invasive proposed....
The mitochondrial uncoupling protein of brown adipose tissue (UCP1) was expressed in skeletal muscle and heart transgenic mice at levels comparable with the amount found mitochondria. These have a lower body weight, when related to food intake energy expenditure are increased. A specific reduction mass observed but varied according contractile activity muscles. Heart soleus unaffected, indicating that muscles undergoing regular contractions, therefore continuous ATP production, protected. In...
Theranostics combines therapeutic and diagnostic or drug deposition monitoring abilities of suitable molecules. Here we describe the first steps building an alkoxyamine-based theranostic agent against cancer. The labile alkoxyamine ALK-1 (t(1/2) = 50 min at 37 °C) cleaves spontaneously to generate (1) a highly reactive free alkyl radical used as agents induce cell damages leading death (2) stable nitroxide contrast for Overhauser-enhanced magnetic resonance imaging (OMRI). toxicity was...
Abstract In vivo investigations of enzymatic processes using non‐invasive approaches are a long‐lasting challenge. Recently, we showed that Overhauser‐enhanced MRI is suitable to such purpose. A β‐phosphorylated nitroxide substrate prototype exhibiting keto–enol equilibrium upon activity has been prepared. Upon hydrolysis, large variation the phosphorus hyperfine coupling constant (Δ P =4 G) was observed. The activities several enzymes were conveniently monitored by electronic paramagnetic...
There is an increasing interest in developing novel imaging strategies for sensing proteolytic activities intact organisms vivo . Overhauser‐enhanced MRI (OMRI) offers the possibility to reveal proteolysis of nitroxide‐labeled macromolecules thanks a sharp decrease rotational correlation time nitroxide moiety upon cleavage. In this paper, concept illustrated at 0.2 T using elastin orally administered mice. vitro , derivative was OMRI‐visible and gave rise high Overhauser enhancements...
Background: Metabolic alterations in skeletal muscle associated with malnutrition and the potential reversibility of such during refeeding are not fully understood. Objective: We characterized early changes malnourished, hospitalized elderly subjects. Design: Muscle function, metabolism, mass were evaluated 24 clinically stable patients (11 malnourished) by using isokinetic plantar flexor torque measurements nuclear magnetic resonance (NMR) imaging for medial gastrocnemius assessment 31P 13C...
Abstract Purpose To examine the ability of TrueFISP imaging for evaluating tumor size in mouse brain at high field. Materials and Methods Brains healthy glioma‐implanted nude mice were imaged 4.7T 9.4T. 3D was performed with T2w‐RARE. Radiofrequency pulses alternating phase sum‐of‐squares reconstruction method used imaging. The contrast‐to‐noise ratio (CNR) evaluated tumoral regions. Results Banding artifact correctly suppressed images thanks to used. signal‐to‐noise (SNR) CNR comparable...
Overhauser‐enhanced MRI (OMRI) offers the potentiality of detecting low‐concentrated species generated by specific biological processes. However molecular imaging applications OMRI need significant improvement in spatial localization. Here it is shown that 3D‐OMRI a free radical injected tumor‐bearing mice can be performed at high anatomical resolution constant field. A 30 mm cavity operating 5.43 GHz was inserted C‐shaped magnet for proton 0.194 T. Nude with or without brain‐implanted C6...
A knowledge of the spatial localization cell vehicles used in gene therapy against glioma is necessary before launching therapy. For this purpose, MRI tracking performed by labeling with contrast agents. In context, goal study was to follow noninvasively chemoattraction therapeutic microglial cells a human model triggering Silica nanoparticles grafted gadolinium were label microglia. These vehicles, expressing constitutively thymidine kinase suicide fused green fluorescent protein gene,...
Staphylococcal δ‐toxin, a 26‐residue amphiphilic peptide is lytic for cells and phospholipid vesicles assumed to insert as an amphipathic helix oligomerize in membranes. For the first time, relationship between these properties toxin structure investigated by means of eight synthetic peptides, one identical sequence natural toxin, five analogues two shorter peptides corresponding residues 1–11 11–26. These were designed Edmundson wheel axial projection order maintain: (a)...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructural characterization of membrane insertion M13 procoat, coat, and Pf3 coat proteinsE. Thiaudiere, M. Soekarjo, E. Kuchinka, A. Kuhn, H. VogelCite this: Biochemistry 1993, 32, 45, 12186–12196Publication Date (Print):November 1, 1993Publication History Published online1 May 2002Published inissue 1 November 1993https://pubs.acs.org/doi/10.1021/bi00096a031https://doi.org/10.1021/bi00096a031research-articleACS PublicationsRequest reuse...
Abstract Blood flow velocity is a functional parameter of fundamental importance in diagnosis and follow‐up various vascular diseases. Vascular pathologies can be efficiently studied animal models, especially small rodents. ECG‐gated magnetic resonance imaging (MRI) assessment blood animals challenge because limited spatial resolution high‐frequency physiological parameters. Here it shown that bright‐blood cine‐3D‐MRI method used to measure at specific times the cardiac cycle mouse pulmonary...
Hypoxic states of the cardiovacular system are undoubtedly associated with most frequent diseases modern time. Therefore, understanding hypoxic resistance encountered after physiological adaptation such as chronic hypoxia, is crucial to better deal insult. In this study, we examine role energetic modifications induced by hypoxia (CH) in higher tolerance oxygen deprivation.
Abstract Proton magnetization transfer contrast (MTC) imaging, using continuous wave off‐resonance irradiation, was performed on the rat brain in vivo at 4.7 Tesla. The observed MTC studied three different regions: corpus callosum , basal ganglia, and temporal lobe. By systematically varying offset frequency amplitude of RF signal intensities for each region interest were modeled a system including free water pool protons with restricted motions (R. M. Henkelman, X. Huang, Q. Xiang, G. J....
Heart contraction is characterized by the absence of changes in energetic intermediates response to a large increase activity. Until now no experimental approach could address this question concerning intact beating heart. Ca(2+) plays crucial role excitation-contraction coupling, and vitro studies have evidenced that may also directly activate mitochondrial oxidative phosphorylation. We applied our new situ modular control regulation analysis on isolated rat heart perfused under two...