A5068750276- Insect and Pesticide Research
- Plant and animal studies
- Insect and Arachnid Ecology and Behavior
- Gut microbiota and health
- Bacteriophages and microbial interactions
- SARS-CoV-2 detection and testing
- Salivary Gland Disorders and Functions
- Insect symbiosis and bacterial influences
- Bee Products Chemical Analysis
- Biosensors and Analytical Detection
- Viral gastroenteritis research and epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Plant Virus Research Studies
- Oral microbiology and periodontitis research
- Cystic Fibrosis Research Advances
- Respiratory viral infections research
- Advanced biosensing and bioanalysis techniques
- Genetics, Aging, and Longevity in Model Organisms
- Pediatric health and respiratory diseases
- Tryptophan and brain disorders
- Metabolomics and Mass Spectrometry Studies
- Mycobacterium research and diagnosis
- Insect-Plant Interactions and Control
- Birth, Development, and Health
- Insect Utilization and Effects
University of California, Irvine
2019-2024
University of California, Riverside
2017-2024
Bioscience Research
2023-2024
UCLA Health
2017
California State Polytechnic University
2013
Abstract The honey bee, Apis mellifera , pollinates a wide variety of essential crops in numerous ecosystems around the world but faces many modern challenges. Among these, microsporidian pathogen Nosema ceranae is one primary detriments to bee health. infects gut, which harbors highly specific, coevolved microbiota heavily involved immune function and nutrition. Here, we extend previous work investigating interactions between gut microbiome N . by studying experimentally infected bees that...
Municipal wastewater provides an integrated sample of a diversity human-associated microbes across sewershed, including viruses. Wastewater-based epidemiology (WBE) is promising strategy to detect pathogens and may serve as early warning system for disease outbreaks. Notably, WBE has garnered substantial interest during the coronavirus 2019 (COVID-19) pandemic track burden through analyses severe acute respiratory syndrome 2 (SARS-CoV-2) RNA. Throughout COVID-19 outbreak, tracking SARS-CoV-2...
Bumble bees are important and widespread insect pollinators who face many environmental challenges. For example, exposed to the metalloid selenate when foraging on pollen nectar from plants growing in contaminated soils. As it has been shown that microbiome of animals reduces toxicity, we assayed ability bee increase survivorship against challenge. We uninoculated or microbiota-inoculated Bombus impatiens workers a field-realistic dose 0.75 mg l-1 found survive slightly but significantly...
Honey bees are important insect pollinators used heavily in agriculture and can be found diverse environments. Bees may encounter toxicants such as cadmium selenate by foraging on plants growing contaminated areas, which result negative health effects. known to have a simple consistent microbiome that conveys many benefits the host, toxicant exposure impact this symbiotic microbial community. We 16S rRNA gene sequencing assay effects sublethal treatments had over 7 days both significantly...
Gram-stain-positive, rod-shaped, non-spore forming bacteria have been isolated from flowers and the guts of adult wild bees in families Megachilidae Halictidae. Phylogenetic analysis 16S rRNA gene indicated that these belong to genus Lactobacillus, are most closely related honey-bee associated Lactobacillus kunkeei (97.0 % sequence similarity) apinorum similarity). analyses genes six single-copy protein coding genes, situ silico DNA-DNA hybridization, fatty-acid profiling differentiates...
Abstract INTRODUCTION Emerging evidence links changes in the gut microbiome to late‐onset Alzheimer's disease (LOAD), necessitating examination of AD mouse models with consideration microbiome. METHODS We used shotgun metagenomics and untargeted metabolomics study human amyloid beta knock‐in (hAβ‐KI) murine model for LOAD compared both wild‐type (WT) mice a early‐onset (3xTg‐AD). RESULTS Eighteen‐month female (but not male) hAβ‐KI microbiomes were distinct from WT microbiomes, genotype...
Summary High temperatures (e.g., fever) and gut microbiota can both influence host resistance to infection. However, effects of temperature‐driven changes in on parasites remain unexplored. We examined the temperature dependence infection bacterial communities bumble bees infected with trypanosomatid parasite Crithidia bombi . Infection intensity decreased by over 80% between 21 37°C. Temperatures peak were lower than predicted based growth vitro , consistent mismatches thermal performance...
Bumblebees (Bombus spp.) are important and widespread insect pollinators, but the act of foraging on flowers can expose them to harmful pesticides chemicals such as oxidizers heavy metals. How these compounds directly influence bee survival indirectly affect health via gut microbiome is largely unknown. As toxicants in floral nectar pollen take many forms, we explored genomes bee-associated microbes for their potential detoxify cadmium, copper, selenate, neonicotinoid pesticide imidacloprid,...
Microbiome research is becoming a mature field with wealth of data amassed from diverse ecosystems, yet the ability to fully leverage multi-omics for reuse remains challenging. To provide view into researchers’ behavior and attitudes towards reuse, we surveyed over 700 microbiome researchers evaluate sharing challenges. We found that many are impeded by difficulties metadata records, challenges processing bioinformatics, problems repository submissions. also explored cost constraints at each...
Diseases caused by viruses in the genus Tobamovirus cause crop losses around world. As with other viruses, mutation occurring virus’s genomes can have functional consequences and may alter viral infectivity.
Human oral microbial communities are diverse, with implications for and systemic health. Oral change over time; thus, it is important to understand how healthy versus dysbiotic microbiomes differ, especially within between families. There also a need the microbiome composition changed an individual including by factors such as environmental tobacco smoke (ETS) exposure, metabolic regulation, inflammation, antioxidant potential. Using archived saliva samples collected from caregivers children...
Given limited resources for motility, sperm cell activation must be precisely timed to ensure the greatest likelihood of fertilization. Like those most species, C. elegans become active only after encountering an external signaling molecule. Activation coincides with spermiogenesis, final step in spermatogenesis, when spherical spermatid undergoes wholesale reorganization produce a pseudopod. Here, we describe gene involved activation, spe-46. This was identified suppressor screen...
Significance Rising temperatures, drought, and growing human populations are increasing demand for reclaimed wastewater agricultural use. However, often contains biologically active, pseudopersistent pharmaceuticals, even after passage through a water treatment facility. We determined that the biology, life histories, microbial communities of an pest insect were altered when reared on artificial diets or plants irrigated by these chemicals. In this study, pharmaceuticals translocated have...
Drought, rising temperatures, and expanding human populations are increasing water demands. Many countries extending potable supplies by irrigating crops with wastewater. Unfortunately, wastewater contains biologically active, long-lived pharmaceuticals, even after treatment. Run-off from farms treatment plant overflows contribute high concentrations of pharmaceuticals to the environment. This study assessed effects common on a cosmopolitan saprophagous insect, Megaselia scalaris (Diptera:...
Mounting evidence suggests that microbes found in the pollen provisions of wild and solitary bees are important drivers larval development. As these also known to be transmitted via environment, most likely from flowers, diet breadth a bee may affect diversity identity occur its provisions. Here, we tested hypothesis that, due importance floral transmission microbes, affects provision microbial community composition. We collected at four sites polylectic Osmia lignaria oligolectic...
Sequencing wastewater may be useful for detecting pathogens and assaying microbial water quality. We concentrated, extracted, sequenced nucleic acids from 17 composite influent samples spanning seven southern California treatment facilities in May 2020. Bacteria were the most proportionally abundant taxonomic group present, followed by viruses archaea.
Wastewater-based epidemiology (WBE) is useful for detecting pathogen prevalence and may serve to effectively monitor diseases across broad scales. WBE has been used throughout the COVID-19 pandemic track disease burden through quantifying SARS-CoV-2 RNA present in wastewater. Aside from case load estimation, being assay viral genomic diversity emerging potential variants. Here, we a study which sequenced extracted sewage influent obtained eight wastewater treatment plants representing 16...
Abstract Human metabolism is complex, and impacted by genetics, cohabitation, diet, health, environmental inputs. As such, we applied untargeted LC-MS metabolomics to 1425 saliva samples from a diverse group of elementary school-aged children their caregivers collected during the Family Life Project, which 1344 were paired into caregiver/child dyads. We compared metabolomes within between homes, performed population-wide “metabotype” analyses, measured associations metabolites salivary...