Significance Oral squamous cell carcinoma (OSCC) is characterized by high morbidity and mortality, few therapeutic options. Here, we show that the expression of B7 Homolog 3 (B7-H3) significantly up-regulated in tumor tissue OSCC patients correlated with increased size poor survival rate. Comparing N-glycans B7-H3 from Ca9-22 oral cancer cells Smulow–Glickman (SG) normal cells, also found glycans contain terminal α-galactose are more diverse higher fucosylation better interaction immune...

Reptiles and fish have robust regenerative powers for tooth renewal. However, extant mammals can either renew their teeth one time (diphyodont dentition) or not at all (monophyodont dentition). Humans replace milk with permanent then lose ability Here, we study renewal in a crocodilian model, the American alligator, which has well-organized similar to but still undergo life-long Each alligator is complex family unit composed of functional tooth, successional dental lamina. Using multiple...

The purpose of this study was to explore the influences cyclic mechanical stretching on mRNA expressions tendon/ligament-related and osteoblast-specific marker genes in human MSCs seeded onto a collagen type I-coated surface. stretch-induced mesenchymal stem cell protein (MSCP), matrix metalloproteinase-3 (MMP-3), related tendon/ligament cells (type I collagen, III tenascin-C) those typical osteoblasts (core binding factor alpha 1 (Cbfa1), alkaline phosphatase (ALP), osteocalcin (OCN)) were...

Stem cell-based therapies for degenerative disorders and injuries are promising in the new era. Multipotential mesenchymal stem cells (MSCs) from bone marrow (BM) on leading edge because they easy to expand culture while maintaining their multilineage potential. In vitro assessment of chondrogenic osteogenic potentials cultured MSCs has been established, BM used those experiments was exclusively healthy donors via iliac crest aspiration. It is unknown whether human obtained femurs also...

Human mesenchymal stem cells (hMSCs) are characterized by their abilities to differentiate into different lineages, including osteoblasts. Besides soluble factors, mechanical strain and extracellular matrix (ECM) proteins play important roles in osteogenic differentiation of hMSCs. However, interactions between them still not fully understood. The purpose this study was investigate the combined effects insoluble chemical factors (ECM vs. cyclic stretching) driving hMSCs differentiation. To...

There are still no effective therapies for hyposalivation caused by irradiation. In our previous study, bone marrow stem cells can be transdifferentiated into acinar-like in vitro. Therefore, we hypothesized that transplantation with or may help functional regeneration of salivary glands. Bone were labeled nanoparticles and directly co-cultured acinar to obtain cells. total, 140 severely combined immune-deficiency mice divided 4 groups cell therapy experiments: (1) normal mice, (2) receiving...

A high mechanical strength and biomimetic scaffold is electrospun from a blend of polyurethane ethyl cellulose, being promising in applications for therapeutic purposes as cardiac graft reconstructing or regeneration damaged myocardium.

The flowable resin composite, Tetric Flow, was used to measure microstrain and degree of conversion after hardening with each three curing machines: XL3000(XL) for 10, 20, 30, 40 s; Optilux 501 using conventional mode (OC) s, as well boost (OB, 10 s) ramp modes (OR, 20 s); LEDemetron (LEDe) s. emitted power density spectral distribution the light units were also measured. LEDe output energy spectrum centralized between 425 490 nm, which encompasses excited wavelength camphorquinone. produced...

Abstract The purpose of this study is to use a tissue engineering approach for tooth regeneration. swine dental bud cells (DBCs) were isolated from the developing mandibular teeth, expanded in vitro , and cultured onto cylinder scaffold gelatin‐chrondroitin‐hyaluronan‐tri‐copolymer (GCHT). After culturing DBCs/GCHT was autografted back into original alveolar socket. Hematoxylin eosin (H&E) staining combined with immunohistochemical applied identification regenerated structure. 36‐week...

The fibrillar architecture in the general matrix of softened cartilage has been compared with that normal using both Nomarski light microscopy and transmission electron combined stereoscopic reconstruction. A pseudorandom network developed from an overall radial arrangement collagen fibrils is most fundamental ultrastructural characteristic matrix. This, turn, provides efficient entrapment system for swelling proteoglycans. Conversely, distinctive feature presence parallel relatively...

Articular cartilage is known to have poor healing capacity after injury. Autologous chondral grafting remains the mainstay treat well-defined, full-thickness, symptomatic defects. We demonstrated utilization of gelatin microbeads deliver autologous chondrocytes for in vivo generation. Chondrocytes were harvested from left forelimbs 12 Lee-Sung pigs. The cells expanded monolayer culture and then seeded onto or monolayer. Shortly before implantation, cell-laden beads mixed with collagen type I...

Background: frail older people often require tailored rehabilitation in order to remain at home, especially following a period of hospitalisation. Restorative care services aim enhance an person's ability improve physical functioning, either home or residential but evidence their effectiveness is limited. Objective: evaluate the restorative service on institutional-free survival and health outcomes referred for needs assessment New Zealand. Methods: randomised controlled trial usual 105 risk...

The collagen fibrillar architectures in the general matrix of cartilage slices removed from both normal and osteoarthritic femoral heads were examined by differential interference light microscopy scanning electron microscopy. Whereas contained a finely differentiated pseudo‐random weave fibrils developed an interconnected array radial elements, was characterised presence structurally distinct regions consisting strongly aligned bundles associated intense tangles or ‘knotted’ features....

3D-printing technology is an important tool for the bone tissue engineering (BTE). The aim of this study was to investigate interaction polycaprolactone (PCL) scaffolds and modified mesh PCL coated with beta TCP (PCL/β-TCP) MG-63. This used fused deposition modeling (FDM) technique 3D printing fabricate thermoplastic polymer composite scaffolds. Scaffold structure coating quality were observed under a scanning electron microscope (SEM). MG-63 cells injected attached mesh-manufactured...

The swelling behaviour of the general matrix both normal and abnormally softened articular cartilage was investigated in context its relationship to underlying subchondral bone, surface, with respect primary structural directions represented strongly anisotropic collagenous architecture. Swelling behaviours were compared by subjecting tissue specimens under different modes constraint a high bathing solution distilled water comparing changes imaged at macroscopic, microscopic ultrastructural...

A reliable method to eradicate the bacteria of residual carious dentin has not yet been developed. The aim this study was evaluate antibacterial effect a diode laser on Streptococcus mutans through different thickness (500, 1,000, and 2,000 microm) human dentin. thermal irradiation also investigated.Dentin specimens were inoculated with 2 microl S. one side irradiated by other power output ranging from 0.5 7 W. tip swept whole area mm x 3 at speed about 10 mm/second total time 30 seconds....

Abstract Background Three‐dimensional (3D) printing has become an available technology to fabricate customized tissue engineering scaffolds with delicate architecture. This exploratory study aimed evaluate the potential of a 3D‐printed hydroxyapatite‐based scaffold as biomaterial for obtaining guided bone regeneration (GBR) in vivo. Methods Scaffolds composed 90% hydroxyapatite and 10% poly(lactic‐co‐glycolic acid) were printed using microextrusion process fit 4 mm diameter 0.5 thick...