Abstract To determine whether the c‐Ha‐ ras oncogene plays a role in initiation of mammary carcinogenesis, an immortalized human breast epithelial cell line, MCF‐10A, was transfected with plasmid vector pHo6T1 containing T24 Ha‐ and aminoglycoside phosphotransferase gene, which confers resistance to geneticin. Transfected cells exhibited altered pattern growth tridimensional morphology collagen gel. They also anchorage‐independent loss requirement for hormones epidermal factor; addition,...

Abstract Mast cell tumours ( MCTs ) are the most common cutaneous of dogs, however rarely they can arise from oral mucosa. This subset MCT is reported to demonstrate a more aggressive clinical course than those on haired skin and authors hypothesised that dogs with oral, mucosal would have high incidence local lymph node metastasis at presentation this be negative prognostic factor. An additional hypothesis was mitotic index (MI) prognostic. retrospective study examines 33 arising The...

ABSTRACT Early‐delayed side effects (EDSEs) following treatment of canine intracranial meningiomas with 1–3‐fraction stereotactic radiation therapy (SRT) can cause worsening neurologic signs, and one potential method mitigating this toxicity is reducing the dose per fraction. Twenty dogs imaging‐diagnosed telephone follow‐up at least 6 months received a protocol Gy × 5, daily (30 Gy). A ‘possible EDSE’ was defined as mental dullness, exacerbation existing signs or new occurring within 1‐4...

The aim of the study was to report outcome treatment 97 dogs with lymphoma that received a multi-agent chemotherapy protocol containing epirubicin as primary anthracycline. Seventy-five 25-week no maintenance phase whilst 22 phase. Complete response rate 96% and time first relapse (TTR) overall survival (OS) for all were 216 342 days, respectively. Dogs T-cell those classified WHO substage b had significantly poorer OS times TTR. well tolerated toxicity similar doxorubicin-containing...

To assess response and outcome in dogs with stage 4 anal sac apocrine gland adenocarcinoma (ASAGA) treated toceranib phosphate as the sole chemotherapeutic agent.Retrospective case series.15 client-owned ASAGA between March 2013 June 2017.Medical records were reviewed, data collected included signalment, clinical signs, results of physical examinations diagnostic procedures, treatments, response, follow-up information, outcomes. Adverse events to treatment assessed according standard...

The aim of the study was to evaluate if thymidine kinase (TK) correlated with duration first remission (DFR) or survival in dogs lymphoma and initial TK levels stage substage; also assess level at diagnosis immunophenotype. assayed 73 treatment naïve lymphoma, then again after treatment; 47% had an above reference interval. Dogs B‐cell higher activities than T‐cell lymphoma. were not disease activity prior associated DFR survival. Where elevated diagnosis, it fell into range during...

A ten‐year‐old, female‐entire English springer spaniel presented with a large intra‐abdominal mass but no other clinical signs. Gastrointestinal stromal tumour of the caecum widespread abdominal metastasis was confirmed. Treatment toceranib phosphate resulted in complete response, despite absence exon‐8 or exon‐11 c‐kit mutation. There evidence recurrence nine months after diagnosis.

Objectives To assess the prevalence of gastrointestinal toxicity in dogs receiving chemotherapy with vincristine and cyclophosphamide efficacy maropitant citrate ( Cerenia™ , Zoetis) reducing these events. Methods Dogs or were randomised to either receive not period immediately after treatment for 4 days afterwards. Owners completed a diary adverse events following treatment. Results Adverse occurred 40/58 (69%) group. Most mild included: lethargy (62%), appetite loss (43%), diarrhoea (34%)...

Anal sac adenocarcinoma is uncommon in cats. We report the outcome of multi-modality therapy two cats (surgery, definitive radiotherapy and systemic chemotherapy) surgery alone All received surgical excision primary tumour followed by carboplatin chemotherapy cases. Both that underwent multimodal developed distant metastatic disease one recurrence tumour. One cat with incomplete margins also rapid recurrence. Overall survival times were 89, 161 169 days. had complete still alive without 425...

Multi‐agent chemotherapy (vincristine, epirubicin and prednisolone) including either cyclophosphamide (CEOP) or lomustine (LEOP) was given as first‐line to treatment‐naïve canine lymphoma patients with measurable, high grade T‐cell (HGTCL). All responded CEOP LEOP. Toxicity typical of multi‐agent protocols 25% dogs receiving exhibited mild‐to‐moderate ALT elevation 29% 3 4 neutropenia. Median progression‐free survival (100 versus 269 days) overall (155 327 were significantly higher in LEOP...

Epitheliotropism is an important diagnostic feature of cutaneous epitheliotropic lymphoma and canine histiocytoma; however, although noted in certain feline mastocytic diseases, it has not been considered a mast cell tumor. In this study, 3 tumors had invasion neoplastic cells into the epidermis follicular epithelium. This unusual histologic finding was characterized by infiltrates individual clusters stratum basale spinosum. The origin these documented demonstration metachromasia with...

Nineteen cats with relapsed high-grade/large-cell lymphoma were treated dexamethasone, melphalan, actinomycin-D and cytarabine (DMAC). All had received Cyclophosphamide, Vincristine, Prednisolone (COP) as first-line chemotherapy most at least 2 prior rescue agents 14 of 19 having both epirubicin lomustine. Five (26%) exhibited a response (defined an improvement or resolution tumour-associated clinical signs/tumour volume, complete/partial response) to though no patients more than cycles...

Temozolomide ( TMZ ) is an alkylating agent previously used in conjunction with doxorubicin DOX to treat dogs relapsed lymphoma. However, there are very limited data for this drug when as single agent. The aim of retrospective study was evaluate the efficacy and toxicity multicentric lymphoma that failed multi‐agent chemotherapy protocols, compare outcome a group receiving same combination . Twenty‐six patients were included 11 / group. Responses evaluated via review medical records. overall...

To summarise the clinical presentation and outcomes in a series of miniature schnauzers diagnosed with histiocytic sarcoma.Retrospective review medical records sarcoma between 2008 2019 at two referral centres UK. Signalment, signs initial presentation, imaging results clinico- histopathological findings, treatment type outcome were recorded. Progression-free survival overall time calculated.Thirty dogs included. Twenty-four 29 undergoing thorax had lung and/or mediastinal involvement. The...

Paraneoplastic syndromes (PNSs) can be described as the indirect effects of tumours, typically due to tumour production and release biologically active substances such hormones, growth factors cytokines. PNSs are sometimes first evidence a neoplastic disease; therefore, knowledge these their associated types help in early diagnosis. should resolve with treatment underlying and, conversely, may return recurrence tumour. In some cases, they cause more significant morbidity than itself, so must...

Background The Flat‐coated Retriever (FCR) is a breed at‐risk for histiocytic sarcoma (HS). A haemophagocytic form of HS (HPHS) occurs in the spleen ± other sites such as bone marrow, and CD11d+ disease macrophage origin. Patients with HPHS typically present regenerative anaemia. Lomustine well‐accepted, first‐line chemotherapy dogs HS, but its specific utility has not been previously reported. Methods cohort 10 presumptively diagnosed via splenic marrow or liver aspiration cytology were...

The DMAC protocol (dexamethasone, melphalan, actinomycin-D, cytarabine) has been evaluated in American studies for the treatment of relapsed canine lymphoma, comparing similarly to other rescue protocols. aim this study was evaluate efficacy and toxicity DMAC, a larger UK cohort resistant lymphomas. Medical records dogs with non-Hodgkin high-grade lymphomas that received as were reviewed from 2007 2017. Response, time initiation discontinuation (TTD) (Veterinary Cooperative Oncology Group...

Abstract/Summary Bacterial vaginosis is a common condition among women of reproductive age and associated with potentially serious side-effects, including an increased risk preterm birth. Recent advancements in microbiome sequencing technologies have produced novel insights into the complicated mechanisms underlying bacterial given rise to new methods diagnosis. Here we report on validation quantitative, molecular diagnostic algorithm based relative abundances ten pathogenic bacteria four...

Abstract Thirty dogs with macroscopic plasma cell tumours (PCTs) were treated radiation therapy (RT). Twelve patients palliative‐intent prescriptions (range, 4‐10 Gy/fraction (median, 7 Gy/fraction) for a total dose of 20 to 35 Gy (median 30 Gy). Eighteen received definitive‐intent 3.0‐4.2 3 42 54 48 Involved sites included the oral cavity, skin, multiple myeloma (MM)‐associated lytic bone lesions, (solitary osseous plasmacytoma; SOP), nasal larynx, retrobulbar space, lymph node and rectum....