A5000747807- Cancer Genomics and Diagnostics
- Gastrointestinal Tumor Research and Treatment
- Thyroid Cancer Diagnosis and Treatment
- Genomic variations and chromosomal abnormalities
- Epigenetics and DNA Methylation
- Gastrointestinal disorders and treatments
- Sarcoma Diagnosis and Treatment
- Glioma Diagnosis and Treatment
- Prenatal Screening and Diagnostics
- Meningioma and schwannoma management
- Gastric Cancer Management and Outcomes
- Renal and related cancers
- Uterine Myomas and Treatments
- Metastasis and carcinoma case studies
- Cancer, Hypoxia, and Metabolism
- Head and Neck Surgical Oncology
- Metabolism, Diabetes, and Cancer
- Neuroblastoma Research and Treatments
- Bioinformatics and Genomic Networks
- Genetic factors in colorectal cancer
- Lung Cancer Treatments and Mutations
- Cancer-related molecular mechanisms research
- Brain Metastases and Treatment
- Asthma and respiratory diseases
- Cancer-related Molecular Pathways
King Abdulaziz University
2015-2024
University of Minnesota Rochester
2017
King Abdulaziz City for Science and Technology
2015-2017
Medical University of South Carolina
2017
King Fahd Medical City
2017
Brigham and Women's Hospital
2017
Harvard University
2017
Boston Children's Hospital
2017
University of Mississippi Medical Center
2017
Jackson Memorial Hospital
2017
Breast cancer brain metastases (BCBM) develop in about 20-30% of breast (BC) patients. BCBM are associated with dismal prognosis not at least due to lack valuable molecular therapeutic targets. The aim the study was identify new biomarkers and targets by using complementary state-of-the-art techniques.We compared array expression profiles three 16 non-brain metastatic BC primary tumors (prBT) a false discovery rate (FDR) p < 0.05 fold change (FC) > 2. Biofunctional analysis conducted on...
Abstract To model the cytogenetic evolution in gastrointestinal stromal tumour (GIST), an oncogenetic tree was reconstructed using comparative genomic hybridization data from 203 primary GISTs (116 gastric and 87 intestinal GISTs, including 151 newly analysed cases), with follow‐up available 173 cases (mean 40 months; maximum 133 months). The identified three major pathways: one initiated by − 14q, 1p, another 22q. 14q pathway mainly characterized tumours predominantly stable karyotypes more...
Follicular variant of papillary thyroid carcinoma (FVPTC) and follicular adenoma (FA) are histologically closely related tumors differential diagnosis remains challenging. RNA expression profiling is an established method to unravel molecular mechanisms underlying the histopathology diseases. BRAF mutational status was by direct sequencing hotspot region exon 15 in six FVPTCs seven FAs. Whole-transcript arrays were employed generate profiles FVPTCs, FAs normal tissue samples. The threshold...
The aim of the current study was to examine prognostic relevance CDKN2A tumor suppressor pathway in gastrointestinal stromal tumors (GIST).We determined mRNA expression p1(INK4A), p14(ARF), CDK4, RB1, MDM2, TP53, and E2F1 by quantitative reverse transcription-PCR 38 cases GISTs correlated findings with clinicopathologic factors, including mutation analysis KIT PDGFRA.The k-means cluster yielded three subgroups distinct patterns pathway. low transcripts p16(INK4A) p14(ARF) but high were...
Cardiovascular disease is the leading cause of morbidity/mortality worldwide. Metformin first therapy offering cardioprotection in type 2 diabetes and non-diabetic animals with unknown mechanism. We have shown that metformin improves angiogenesis via affecting expression growth factors/angiogenic inhibitors CD34⁺ cells under hyperglycemia-hypoxia. Now we studied direct effect physiological dose on human umbilical vein endothelial (HUVEC) conditions mimicking hypoxia-hyperglycemia. HUVEC...
Cardiovascular disease (CVD) is the leading cause of morbidity and mortality in patients with diabetes mellitus (DM). To identify most effective treatment for CVD, it paramount to understand mechanism behind cardioprotective therapies. Although metformin has been shown reduce CVD Type-2 DM clinical trials, underlying remains unexplored. CD34+ cell-based therapies offer a new approach CVD. The aim this study was investigate effect on angiogenic properties cells under conditions mimicking...
Hashimoto's thyroiditis (HT) is present in the background of around 30% papillary thyroid carcinomas (PTCs). The genetic predisposition effect this autoimmune condition not thoroughly understood. We analyzed microarray expression profiles 13 HT, eight PTCs with (w/) coexisting six without (w/o) micro (mPTCs), and three normal (TN) samples. Based on a false discovery rate (FDR)-adjusted p-value ≤ 0.05 fold change (FC) > 2, four comparison groups were defined, which HT vs. TN; PTC w/ w/o mPTC...
The molecular etiology of thyroid carcinoma (TC) and other diseases which may present malignant precursor lesions is not fully explored yet. purpose this study was to estimate frequency, type clinicopathological value BRAF exon 15 mutations in different types cancerous non-cancerous originating an ethnically diverse population.BRAF sequenced 381 cases including Hashimoto´s thyroiditis, nodular goiters, hyperplastic nodules, follicular adenomas (FA), papillary TC (PTC), variant PTC (FVPTC),...
Abstract Human umbilical vein endothelial cell (HUVEC)-based gene expression studies performed under hypoxia and/or hyperglycemia show huge potential for modeling response in cardiovascular disease and diabetes. However, such require reference genes that are stable across the whole range of experimental conditions. These have not been comprehensively defined to date. We applied human genome-wide microarrays quantitative real-time PCR (qRT-PCR) on RNA obtained from primary HUVEC cultures were...
Recurrent pregnancy loss (RPL) or recurrent spontaneous abortion is an obstetric complication that affects couples at reproductive age. Previous reports documented a clear relationship between parents with chromosomal abnormalities and both miscarriages infertility. However, limited data available from the Arabian Peninsula which known by higher rates of consanguineous marriages. The main goal this study was to determine prevalence thrombophilic polymorphisms, correlate them RPL...
Breast cancer incidence rates are increasing at an alarming rate among Saudi Arabian females. Most molecular genetic discoveries on breast and other cancers have arisen from studies examining European American patients. However, possibility of specific changes in signature patients diverse ethnic groups remains largely unexplored. We performed transcriptomic profiling surgically-resected tumors 45 based the Western region Arabia using Affymetrix Gene 1.0 ST chip. Pathway biological...
Renal cell carcinoma (RCC) is a seventh ranked malignancy with poor prognosis. RCC lethal at metastatic stage as it does not respond to conventional systemic treatments, and there an urgent need find out promising novel biomarkers for effective treatment. The goal of this study was evaluate the that can be potential therapeutic target predict inhibitors treat RCC.We conducted transcriptomic profiling identify differentially expressed genes associated RCC. Molecular pathway analysis done...
Meningiomas are the most common primary brain tumors bearing in a minority of cases an aggressive phenotype. Although meningiomas stratified according to their histology and clinical behavior, underlying molecular genetics predicting aggressiveness not thoroughly understood. We performed whole transcript expression profiling 10 grade I four II meningiomas, three which invaded brain. Microarray analysis identified deleted colorectal cancer (DCC) as differentially expressed gene (DEG) enabling...
Although the significance of tumour site for estimating malignant potential in gastrointestinal stromal tumours (GISTs) has recently been recognized, site-specific genetic patterns have not to date defined. This study examined 52 c-kit-positive primary GISTs (with a mean follow-up 42.3 months 51 cases) from three different locations (35 gastric, 12 small intestinal, and five colorectal) using comparative genomic hybridization (CGH). In general, correlated with key prognostic factors,...
Thymomas and thymic carcinomas are peculiar epithelial tumors of the anterior mediastinum. They may show aggressive clinical behavior a paradigm for interaction between tumor immune system. So far, adequate functional studies enabling better understanding this malignancy have not been performed, since human thymoma/thymic carcinoma cell lines available. Here, authors describe establishment, characterization analyses from Type B1-thymoma poorly differentiated carcinoma. By...
Most sporadic gastrointestinal stromal tumors (GISTs) occur solitary, whereas a multicentric appearance is suspicious for familial or syndromal setting such as with germline mutations of proto-oncogene tyrosine protein kinase Kit (KIT) platelet derived growth factor receptor alpha (PDGFRA), even metastases. The aim this study was to evaluate whether GISTs are clonal origin. Four patients 1 clinically apparent tumor (mean size 5.6 cm) and 3 further small incidental 0.7 were analysed by...
Meningioma tumors arise in arachnoid membranes, and are the most reported central nervous system (CNS) worldwide. Up to 20% of grade I meningioma reoccur currently predictive cancer stem cells (CSCs) markers for aggressive drug resistant meningiomas scarce. tissues primary cell lines were investigated using whole transcriptome microarray analysis, immunofluorescence staining CSCs (including CD133, Sox2, Nestin, Frizzled 9), treatment with cisplatin or etoposide. Unsupervised hierarchical...