A5027287061- Molecular Biology Techniques and Applications
- Forensic and Genetic Research
- Environmental DNA in Biodiversity Studies
- Genomics and Phylogenetic Studies
- Cancer Genomics and Diagnostics
- RNA and protein synthesis mechanisms
- Gene expression and cancer classification
- Genomic variations and chromosomal abnormalities
- Glycosylation and Glycoproteins Research
- Parasites and Host Interactions
- Carbohydrate Chemistry and Synthesis
- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- Delphi Technique in Research
- RNA modifications and cancer
- Micro and Nano Robotics
- Helminth infection and control
- Parasite Biology and Host Interactions
Reprogenetics
2022-2024
Qiagen (United States)
2024
Illumina (United States)
2016-2017
University of California, San Francisco
2006-2011
QB3
2009
<i>O</i>-GlcNAc is a widespread dynamic carbohydrate modification of cytosolic and nuclear proteins with features analogous to phosphorylation. <i>O-</i>GlcNAc acts critically in many cellular processes, including signal transduction, protein degradation, regulation gene expression. However, the study its specific regulatory functions has been limited by difficulties mapping sites modification. We report methods for direct enrichment identification <i>in vivo O-</i>GlcNAc-modified peptides...
Forensic Investigative Genetic Genealogy, a recent sub discipline of forensic genomics, leverages the high throughput and sensitivity detection next generation sequencing established genetic genealogical approaches to support identification human remains from missing persons investigations investigative lead in violent crimes. To facilitate DNA evidence analysis, ForenSeq® Kintelligence multiplex, consisting 10,230 SNPs, was developed. Design ForenSeq Kit, MiSeq FGx® Sequencing System...
Background Praziquantel (PZQ) is the only widely available drug to treat schistosomiasis. Given potential for resistance, it prudent search novel therapeutics. Identification of anti-schistosomal chemicals has traditionally relied on phenotypic (whole organism) screening with adult worms in vitro and/or animal models disease—tools that limit automation and throughput modern microtiter plate-formatted compound libraries. Methods A partially automated, three-component screen workflow presented...
For human identification purposes, forensic genetics has primarily relied upon a core set of autosomal (and to lesser extent Y chromosome) short tandem repeat (STR) markers that are enriched by amplification using the polymerase chain reaction (PCR) subsequently separated and detected capillary electrophoresis (CE). While STR typing conducted in this manner is well-developed robust, advances molecular biology have occurred over last 15 years, particular massively parallel sequencing (MPS)...
Cilia are motile and sensory organelles with critical roles in physiology. Ciliary defects can cause numerous human disease symptoms including polycystic kidneys, hydrocephalus, retinal degeneration. Despite the importance of these organelles, their assembly function is not fully understood. The unicellular green alga Chlamydomonas reinhardtii has many advantages as a model system for studies ciliary function. Here we describe our initial efforts to build chemical-biology toolkit augment...
The need to identify large numbers of missing people from mass disasters, armed conflicts, and human rights abuses has become increasingly prevalent. DNA methods for disaster victim identification (DVI) typically rely on the postmortem (PM) samples through comparison antemortem (AM) close relatives mitochondrial or short tandem repeat (STR) profiling. There are several limitations these tests: STRs cannot further out than second degree do not always provide full profiles in degraded samples;...
The successful application of Forensic Investigative Genetic Genealogy (FIGG) to the identification unidentified human remains and perpetrators serious crime has led a growing interest in its use internationally, including Australia. Routinely, FIGG relied on generation high-density single nucleotide polymorphism (SNP) profiles from forensic samples using whole genome array (WGA) (∼650,000 or more SNPs) sequencing (WGS) (millions for DNA segment-based comparisons commercially available...
1. Abstract Forensic genetic genealogy (FGG) has primarily relied upon dense single nucleotide polymorphism (SNP) profiles from forensic samples or unidentified human remains queried against online database(s) of known generated with SNP microarrays whole genome sequencing (WGS). In these queries, SNPs are compared to database by locating contiguous stretches shared alleles that allow for detection genomic segments identical descent (IBD) among biological relatives (kinship). This...
Forensic Investigative Genetic Genealogy, a recent sub discipline of forensic genomics, leverages the high throughput and sensitivity detection next generation sequencing established genetic genealogical approaches to support identification human remains from missing persons investigations investigative lead in violent crimes. To facilitate DNA evidence analysis, ForenSeq® Kintelligence multiplex, consisting 10,230 SNPs, was developed. Design ForenSeq Kit, MiSeq FGx® Sequencing System...
Abstract Expanding the paradigm of solid tumor profiling from single-gene testing to comprehensive panels presents many challenges. One such challenges is ability these detect genetic alterations FFPE samples, where DNA low abundance and often heavily compromised. Despite challenges, next-generation sequencing (NGS) offers assess multiple variants simultaneously in an ever-expanding list relevant genes. To that end, Illumina developed a comprehensive, hybrid capture-based NGS assay targeting...
Abstract Recent studies have highlighted the importance of gene fusions and splice variants in solid tumor profiling1. Next-generation sequencing can be an effective means detecting these alterations FFPE samples using RNA rather than DNA, as a single chimeric transcript could result from numerous DNA2. To that end, Illumina developed TruSight® Tumor 1703, comprehensive, hybrid capture-based NGS assay targeting 170 key cancer genes. Along with DNA workflow, includes workflow for...
Abstract Background: As our knowledge of how DNA alterations can drive cancer progression increases, assays that simultaneously detect multiple types variants in a simple and cost-effective manner are becoming increasingly crucial. This holds true copy number variations (CNVs), where evaluation this type variant is an important necessary feature any solid tumor profiling assay. Conventional methods for detecting CNVs such as immunohistochemistry (IHC), fluorescence situ hybridization (FISH),...