A5081304881- Advanced biosensing and bioanalysis techniques
- Nanopore and Nanochannel Transport Studies
- Biosensors and Analytical Detection
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Advanced Biosensing Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Fullerene Chemistry and Applications
- Microfluidic and Bio-sensing Technologies
- Fuel Cells and Related Materials
- Berberine and alkaloids research
- Synthesis and Properties of Aromatic Compounds
- Polymer Surface Interaction Studies
- Semiconductor materials and devices
- Mass Spectrometry Techniques and Applications
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Molecular Sensors and Ion Detection
- Nanoparticle-Based Drug Delivery
- Liver physiology and pathology
- Connective tissue disorders research
- Cannabis and Cannabinoid Research
- Machine Learning in Bioinformatics
- Porphyrin and Phthalocyanine Chemistry
- Metal-Organic Frameworks: Synthesis and Applications
- Inorganic Fluorides and Related Compounds
Sichuan University
2013-2024
China Pharmaceutical University
2014-2016
Chengdu University
2016
Jiangsu Provincial Center for Disease Control and Prevention
2014
University of Florida
2014
West China Second University Hospital of Sichuan University
2011
Chinese Academy of Sciences
2006
Institute of Chemistry
2006
Excessive alcohol consumption induces oxidative stress and lipid accumulation in the liver. Mitochondria have long been recognized as key target for alcoholic liver disease (ALD). Recently, artificial mitochondria-targeted antioxidant MitoQ has used to treat ALD effectively mice. Here, we introduce natural demethyleneberberine (DMB), which found Chinese herb <i>Cortex Phellodendri chinensis</i>. The protective effect of DMB on was evaluated with HepG2 cells acutely/chronically ethanol-fed...
Circulating tumor cells (CTCs) have been utilized in the diagnosis and prognosis of tumor. However, CTC concentration is extremely low to be detected peripheral blood. Many existing methods suffer from either expensive labeling or complex operation. In this study, we constructed a label- enzyme-free sensitive method detect breast cancer CTCs. First all, probe containing cell-specific aptamer complementary single-stranded DNA (trigger P1) were designed. When target are present, binds CTCs...
Sensitive detection of resistance mutation T790 M is great significance for early diagnosis and prognostic monitoring non-small-cell lung cancer (NSCLC). In this paper, we showed a highly sensitive strategy using three-level characteristic current signal pattern in an α-hemolysin nanopore. A probe was designed that formed C-T mismatched base pair with wild-type/P T-T the T790M/P. The T790M/P produced unique presence mercury ions(II): first, T790M-Hg
Abstract The application of cancer theranostics depends on the development multifunctional nanostructured platforms for accurate cell targeting and controlled drug release, imaging, therapy. Herein, a comprehensive, easily fabricated anticancer theranostic platform with high drug‐loading capacity, termed an aptamer‐functionalized calcium carbonate (CaCO 3 ) nanostructure (apt‐CCN), is reported. Flow cytometry confocal fluorescence microscopy studies demonstrate that apt‐CCNs can specifically...
A bicyclic pillar[5]arene derivative fused with a bipyridine side ring, so-called molecular universal joint (MUJ), was synthesized, and the pair of enantiomers resolved by high-performance liquid chromatography enantioresolution. The electrochemiluminescent detection based on ruthenium complex enantiopure MUJ showed excellent chiral discrimination toward certain amino acids.
Demethyleneberberine (DMB) is an essential metabolite of Berberine (BBR) in vivo. Recent reports have revealed multiple novel therapeutic applications BBR. However, the pharmacological activities DMB remain to be elucidated. This study aimed demonstrate hepatoprotective and anti-fibrotic effects both vitro Here we showed that protects against thioacetamide (TAA)-induced hepatic fibrosis mice exhibits a higher safety profile as compared Flow cytometry Western blotting analysis able suppress...
A rapid room-temperature DNA nanowires preparation strategy on the basis of self-replicating catalyzed hairpin assembly (SRCHA) was reported. In this system, three probes (P1, P2, and P3) were well-designed partially hybridize to each other, two split trigger sequences integrated into P1 P3, respectively. When SRCHA initiated by DNA, a series steps based toehold-mediated strand displacement activated, Y shaped (P1-P2-P3) formed. that case, will come close-enough proximity form replicas,...
Alloyed upconversion nanoparticles (aUCNPs, NaYbF4:10% Er@NaYF4) with high Yb3+ doping were designed to brighten luminescence, exploring multicolor patterns of information encryption. The luminescence intensity aUCNPs was 2.3 times higher than that common doped UCNPs reported (dUCNPs, NaYF4:20% Yb, 2% Er@NaYF4). Furthermore, because the (90%), both green and red emissions enhanced, ∼4.2 ∼17.2 stronger 10% Er@NaYF4, respectively. reasons for having a brightened explored by synthesizing four...
One-dimensional DNA polymer chains were obtained based on the catalyzed hairpin assembly and sticky end self-assembly, which led to a signal amplified colorimetric nucleic acid assay.
Abstract Nanoscale transport through nanopores and live-cell membranes plays a vital role in both key biological processes as well biosensing DNA sequencing. Active translocation of these usually needs enzyme assistance. Here we present nanopore derived from truncated helicase E1 bovine papillomavirus (BPV) with lumen diameter c.a. 1.3 nm. Cryogenic electron microscopy (cryo-EM) imaging single channel recording confirm its insertion into planar lipid bilayer (BLM). The BLM allows the passive...
Interface between neuron cells and biomaterials is the key to real-time sensing, transmitting manipulating of activities, which are long-term pursue scientists gain intense research focus recently. It great interest develop a sensor with exquisite sensitivity excellent selectivity for monitoring neurotransmitters transport through single live cell. Sensing techniques including electrode-based methods, optogenetics, nanowire cell penetration systems have been developed monitor activities....
A plasmonic nanoplatform to perform an enzyme-free, naked-eye, and trace discrimination of single-base mutation from fully matched sequence is reported. The showed great potential enhance catalytic hairpin assembly (CHA) amplification efficiency biocatalytic activity hemin/G-quadruplex (DNAzyme). When human immunodeficiency virus (HIV) DNA biomarker was used as the model analyst, a naked-eye detection with high selectivity sensitivity down 10–17 M in whole serum achieved by observing...
A water-soluble covalently linked C60-porphyrin compound–C60Por is reported to show enhanced DNA-cleaving activity. The highly activity expected be ascribed the high water-solubility and affinity of C60Por DNA, as well accessibility charge-separated states C60˙−–Por˙+ formed under radiation.
With the aim of detecting low frequency drug resistant mutation T790M against wild-type sequences, we reported a two-dimensional signal analysis strategy by combining three locked nucleic acids (LNAs)-modified probe (LP15-3t) and an α-HL nanopore. The specific hybridization LP15-3t with generated unique long two-level signals, including characteristic blocking current dwell time. Due to significant time difference (114.2-fold) ranging from 81% 96%, this can simultaneously distinguish...
Ruthenium nanoparticle tris(2,2'-bipyridyl)ruthenium(II) bis(hexafluorophosphate) (Ru(bpy)3(PF6)2, RuNP) was self-assembled on polystyrene (PS) electrospun microfibers. The formation of RuNP is attributed to the sulfonated PS (SPS) microfibers' high adsorptive capability 94% for Ru(bpy)3(2+), as well strong interaction between Ru(bpy)3(2+) and ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate, BMIMPF6). RuNP/SPS microfibers exhibited an enhanced electrochemiluminescence (ECL)...
Abstract The toxic oxidative damage of G‐quadruplexes (G4), linked to neurodegenerative diseases, may arise from their ability bind and oxidatively activate cellular hemin. However, there have been no precise studies on how telomeric G4 enhances the low intrinsic peroxidase activity Herein, a label‐free nanopore‐based strategy was developed explore enhancement mechanism hemin induced by (d(TTAGGG) n ). demonstrated that were simultaneously two different binding modes telomere At...
Identification of single-base mismatches has found wide applications in disease diagnosis, pharmacogenetics, and population genetics. However, there is still a great challenge the simultaneous discrimination mismatch full match. Combined with nanopore electrochemical sensor, shared-stem structure molecular beacon was designed that did not need labeling, but achieved an enhanced signal-to-background ratio ∼104, high thermodynamic stability to bind target sequences, fast hybridization rate....