A5020792358- Lymphoma Diagnosis and Treatment
- DNA Repair Mechanisms
- Telomeres, Telomerase, and Senescence
- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- Cancer-related molecular mechanisms research
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- Plant Virus Research Studies
- Chemokine receptors and signaling
- Moyamoya disease diagnosis and treatment
- Circular RNAs in diseases
- CNS Lymphoma Diagnosis and Treatment
- RNA modifications and cancer
- Sarcoma Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Carcinogens and Genotoxicity Assessment
- Ovarian cancer diagnosis and treatment
- Viral-associated cancers and disorders
- Epigenetics and DNA Methylation
- Plant Genetic and Mutation Studies
- TGF-β signaling in diseases
- Transgenic Plants and Applications
The Maria Sklodowska-Curie National Research Institute of Oncology
2009-2024
DNA damage can activate the oncosuppressor protein ataxia telangiectasia mutated (ATM), which phosphorylates histone H2AX within characteristic foci. Here, we show that ATM undergoes an activating phosphorylation in syncytia elicited by envelope glycoprotein complex (Env) of human immunodeficiency virus-1 (HIV-1) vitro. This was accompanied aggregation discrete nuclear foci also contained phospho-histone H2AX. containing phosphorylated and were detectable present brain or lymph nodes from...
Primary central nervous system lymphoma (PCNSL) is a rare, highly aggressive, extranodal form of non-Hodgkin lymphoma, predominantly diagnosed as primary diffuse large B-cell the (CNS DLBCL). Fast and precise diagnosis PCNSL critical yet challenging. microRNAs, important regulators in physiology pathology are potential biomarkers. In 131 patients with CNS DLBCL non-malignant brain lesions (n-ML), miR-21, miR-19b miR-92a, miR-155, miR-196b, miR-let-7b, miR-125b, miR-9 were examined by RT-qPCR...
Fast and reliable differential diagnosis of Burkitt lymphoma (BL) vs. diffuse large B cell (DLBCL) is major importance for therapeutic decisions patient outcome. Aggressive non-Hodgkin lymphomas (B-NHLs) that do not belong to the abovementioned entities were categorized by current WHO classification as "B-cell lymphoma, unclassifiable, with features intermediate between DLBCL BL" (DLBCL/BL). We have recently described a DLBCL/BL subgroup recurrent chromosome 11q aberrations, resembling BL...
Our knowledge on immortalization and telomere biology is mainly based genetically manipulated cells analyzed before many population doublings post growth crisis. The general view that crisis length (TL) dependent escape from coupled to increased expression of the telomerase reverse transcriptase (hTERT) gene, activity upregulation TL stabilization. Here we have process spontaneous human T cells, regarding pathways involved in senescence regulation. Two Nijmegen breakage syndrome (NBS) cell...
The molecular mechanisms of cell cycle exit are poorly understood. Studies on lymphocytes at after growth factor deprivation have predominantly focused the initiation apoptosis. We aimed to study gene expression profile primary and immortalised IL-2-dependent human T cells forced by withdrawal, before apoptosis could be evidenced. By Affymetrix microarrays HG-U133 2.0 Plus, 53 genes were distinguished as differentially expressed soon IL-2 deprivation. Among those, PIM1, BCL2, IL-8, HBEGF,...
We have previously described gene expression changes during spontaneous immortalization of T-cells, thereby identifying cellular processes important for cell growth crisis escape and unlimited proliferation. Here, we analyze the same model to investigate role genome-wide methylation in process at different time points pre-crisis post-crisis using high-resolution arrays. show that over culture there is an overall accumulation alterations, with preferential increased close transcription start...
Molecular alterations in tumor-adjacent tissues have recently been recognized some types of cancer. This phenomenon has not studied endometrial We aimed to analyze the expression genes associated with cancer progression and metabolism primary samples matched endometria from cancer-free patients uterine leiomyomas. Paired tumors 49 (EC), endometrium 25 leiomyomas uterus, 4 cell lines were examined by RT-qPCR, for MYC, NR5A2, CXCR2, HMGA2, LIN28A, OCT4A, OCT4B, OCT4B1, TWIST1, STK11, SNAI1,...
Nibrin plays an important role in the DNA damage response (DDR) and repair. DDR is a crucial signaling pathway apoptosis senescence. To verify whether truncated nibrin (p70), causing Nijmegen Breakage Syndrome (NBS), involved cell fate upon damage, we used two (S4 S3R) spontaneously immortalized T lines from NBS patients, with founding mutation control line (L5). S4 S3R cells have same level of p70 nibrin, however was able to form more complexes ATM BRCA1. Doxorubicin-induced followed by...
The diagnosis of primary central nervous system (CNS) lymphoma, which is predominantly the diffuse large B-cell lymphoma type (CNS DLBCL), challenging. MicroRNAs (miRs) are gene expression-regulating non-coding RNAs that potential biomarkers. We aimed to distinguish miR expression patterns differentiating CNS DLBCL and non-malignant diseases with tumor presentation (n-ML). Next generation sequencing-based profiling cerebrospinal fluids (CSFs) brain tumors was performed. Sample...
EMSY, a BRCA2-associated protein, is amplified and overexpressed in various sporadic cancers. This the first study assessing clinical impact of its expression polymorphisms on ovarian cancer (OvCa) outcome context chemotherapy regimen used. In 134 frozen OvCa samples, we assessed EMSY mRNA with Reverse Transcription-quantitative PCR, also investigated gene sequence using SSCP and/or PCR-sequencing. Clinical relevance changes DNA was evaluated two subgroups treated either taxane/platinum (TP,...
High-grade B-cell lymphoma with 11q aberration (HGBCL-11q) is a rare germi-nal centre characterised by typical gain/loss pattern on chromo-some but without MYC translocation. It shares some features Burkitt (BL), HGBCLs and germinal centre-derived diffuse large lym-phoma, not otherwise specified (GCB-DLBCL-NOS). Since microRNA expression in HGBCL-11q remains unknown, we aimed to identify compare the mi-croRNA profiles HGBCL-11q, BL GCB-DLBCL-NOS. Next-generation sequencing (NGS)-based profiling of (
Abstract To gain further insight into the molecular events responsible for extended life span and immortalization of human lymphoid cells, we analyzed a series spontaneously immortalized, IL2‐dependent T‐cell lines using cytogenetic techniques. Two cell were derived from normal spleen three patients with Nijmegen breakage syndrome (NBS), recessive disorder characterized by high incidence malignancies. Here show that spontaneous five was associated acquisition copy number gains involving...
Burkitt-like lymphoma with 11q aberration (BLL,11q) is a new entity of germinal center (GCB)-derived, highly aggressive B-cell lymphoma. Clinically and pathomorphologically, BLL,11q resembles Burkitt (BL), but it lacks MYCrearrangements presents proximal gains distal losses in chromosome 11. In the updated 2017 WHO classification, has been recognized as provisional entity. could be promoted to definite lymphomas for this purpose necessary publish many cases possible, more molecular studies...