Iwona K. Rzepecka
A5040696436- Ovarian cancer diagnosis and treatment
- Cancer-related molecular mechanisms research
- BRCA gene mutations in cancer
- RNA Research and Splicing
- Genetic Associations and Epidemiology
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- Cancer Mechanisms and Therapy
- Bioinformatics and Genomic Networks
- Genetic factors in colorectal cancer
- Cancer, Lipids, and Metabolism
- Cancer Immunotherapy and Biomarkers
- DNA Repair Mechanisms
- NF-κB Signaling Pathways
- T-cell and B-cell Immunology
- Cancer-related Molecular Pathways
- 14-3-3 protein interactions
- Immune Cell Function and Interaction
- Systemic Sclerosis and Related Diseases
- Chronic Lymphocytic Leukemia Research
- Epigenetics and DNA Methylation
- Genomics, phytochemicals, and oxidative stress
- Kruppel-like factors research
The Maria Sklodowska-Curie National Research Institute of Oncology
2011-2025
University College London
2013-2016
Stanford University
2016
Lunenfeld-Tanenbaum Research Institute
2016
Women's College Hospital
2016
Mount Sinai Hospital
2016
Radboud University Nijmegen
2016
Radboud University Medical Center
2016
London Women's Clinic
2016
Pomeranian Medical University
2013-2015
HNF1B is overexpressed in clear cell epithelial ovarian cancer, and we observed epigenetic silencing serous leading us to hypothesize that variation this gene differentially associates with cancer risk according histological subtype. Here comprehensively map respect analyse DNA methylation expression profiles across subtypes. Different single-nucleotide polymorphisms associate invasive (rs7405776 odds ratio (OR)=1.13, P=3.1 × 10−10) (rs11651755 OR=0.77, P=1.6 10−8) cancer. Risk alleles for...
Ovarian tumors from two patients, compatible by histological and immunohistochemical criteria with small cell carcinoma of hypercalcemic type (SCCHT) (WT1+, EMA dispersed+, synaptophysin+ or dispersed+), were extensively sampled in order to find clues their histogenesis. Subsequently, foci immature teratoma found both them (in 1/122 3/80 tumor sections). In one case, microfoci yolk sac also present within the area as well background population - primary omental metastasis. We a resemblance...
The introduction of microarray techniques to cancer research brought great expectations for finding biomarkers that would improve patients' treatment; however, the results such studies are poorly reproducible and critical analyses these methods rare. In this study, we examined global gene expression in 97 ovarian samples. Also, validation by quantitative RT-PCR was performed on 30 additional We carried out a number systematic relation several defined clinicopathological features. main goal...
Ovarian cancer is typically diagnosed at late stages, and thus, patients' prognosis poor. Improvement in treatment outcomes depends, least partly, on better understanding of ovarian biology finding new molecular markers therapeutic targets. An unsupervised method data analysis, singular value decomposition, was applied to analyze microarray from 101 samples; then, selected genes were validated by quantitative PCR. We found that the major factor influencing gene expression tumor histological...
Background: The phosphoinositide 3-kinase (PI3K) pathway is activated in multiple cancers. However, the significance of PIK3R1 encoding PI3K regulatory subunit, an inhibitor catalytic subunit encoded by PIK3CA, ovarian cancer development largely unknown. Methods: Here, we investigated genomic alterations and gene expression direct sequencing qPCR methods 197 results were correlated with clinicopathological molecular variables patient outcomes. Results: In addition to mutations (3.5%) allelic...
Abstract Purpose: Chemotherapy resistance remains a major challenge in the treatment of ovarian cancer. We hypothesize that germline polymorphisms might be associated with clinical outcome. Experimental Design: analyzed approximately 2.8 million genotyped and imputed SNPs from iCOGS experiment for progression-free survival (PFS) overall (OS) 2,901 European epithelial cancer (EOC) patients who underwent first-line cytoreductive surgery chemotherapy regardless regimen, subset 1,098 treated ≥4...
SMARCA4 mutations have recently been identified as driving lesions of the ovarian small cell carcinoma hypercalcemic type (SCCHT). Familial occurrence this neoplasm was described previously. We looked for germline alterations in eight patients with SCCHT. DNA extracted from probands’ and their relatives’ blood. The coding sequence, previously found altered all tumors, PCR amplified sequenced DNA. Two carried a heterozygous alteration: c.3760G > T c.2352insG, respectively. analysis next kins...
Deletion of 13q14 is the most common cytogenetic change in chronic lymphocytic leukemia/small lymphoma (CLL/SLL) and detected about 50 % patients by fluorescence situ hybridization (FISH), which can reveal presence del(13)(q14) mono- or biallelic deletion status without information size lost region. Array-comparative genomic (aCGH) single nucleotide polymorphism (SNP) detect submicroscopic copy number changes, loss heterozygosity (LOH) uniparental disomy (UPD) regions. The purpose this study...
DNA repair pathways are potential targets of molecular therapy in cancer patients. The FANCD2, BRIP1, BRCA1/2, and FANCF genes involved homologous recombination repair, which implicates their possible role cell response to DNA-damaging agents. We evaluated a clinical significance pre-treatment expression these at mRNA level 99 primary, advanced-stage ovarian carcinomas from patients, who later received taxane-platinum (TP) or platinum-cyclophosphamide (PC) treatment.Gene was determined with...
Abstract The presence of regulatory T cells (Treg) in solid tumors is known to play a role patient survival ovarian cancer and other malignancies. We assessed inherited genetic variations via 749 tag single-nucleotide polymorphisms (SNP) 25 Treg-associated genes (CD28, CTLA4, FOXP3, IDO1, IL10, IL10RA, IL15, 1L17RA, IL23A, IL23R, IL2RA, IL6, IL6R, IL8, LGALS1, LGALS9, MAP3K8, STAT5A, STAT5B, TGFB1, TGFB2, TGFB3, TGFBR1, TGRBR2, TGFBR3) relation survival. analyzed genotype overall 10,084...
Abstract Background: Ovarian cancer is a leading cause of cancer-related death among women. In an effort to understand contributors disease outcome, we evaluated single-nucleotide polymorphisms (SNP) previously associated with ovarian recurrence or survival, specifically in angiogenesis, inflammation, mitosis, and drug disposition genes. Methods: Twenty-seven SNPs VHL, HGF, IL18, PRKACB, ABCB1, CYP2C8, ERCC2, ERCC1 outcome were genotyped 10,084 invasive cases from 28 studies the Cancer...
Abstract Background Survivin is an inhibitor of apoptosis and a regulator mitotic progression. TP53 protein negative transcriptional survivin. The aim our study was to evaluate the clinical significance survivin expression in advanced stages ovarian cancer with respect status. Methods evaluated immunohistochemically 435 archival samples carcinomas (244 patients were treated platinum/cyclophosphamide-PC/PAC; 191-with taxane-platinum (TP) agents). Univariate multivariate statistical analyses...
p19INK4d (CDKN2D) is a negative regulator of the cell cycle. Little known its role in cancer development and prognosis. We aimed to evaluate clinical significance expression ovarian carcinomas with respect TP53 accumulation status, as well frequency CDKN2D mutations. was evaluated immunohistochemically 445 carcinomas: 246 patients were treated platinum–cyclophosphamide (PC/PAC), while 199 taxane–platinum agents (TP). gene (mRNA) examined 106 carcinomas, mutations 68 tumors. Uni- multivariate...
Abstract Survival in epithelial ovarian cancer (EOC) is influenced by the host immune response, yet key genetic determinants of inflammation and immunity that affect prognosis are not known. The nuclear factor-κB (NF-κB) transcription factor family plays an important role many inflammatory responses, including response to cancer. We studied common inherited variation 210 genes NF-κB 10,084 patients with invasive EOC (5,248 high-grade serous, 1,452 endometrioid, 795 clear cell, 661 mucinous)...
ABSTRACT We report a meta-analysis of breast, prostate, ovarian, and endometrial cancer genome-wide association data (effective sample size: 237,483 cases/317,006 controls). This identified 465 independent lead variants ( P <5×10 −8 ) across 192 genomic regions. Four were >1Mb from previously risk loci for the four cancers an additional 23 variant-cancer associations novel one cancers. Bayesian models supported pleiotropic effects involving at least two 222/465 in 118/192 Gene-level...
EMSY, a BRCA2-associated protein, is amplified and overexpressed in various sporadic cancers. This the first study assessing clinical impact of its expression polymorphisms on ovarian cancer (OvCa) outcome context chemotherapy regimen used. In 134 frozen OvCa samples, we assessed EMSY mRNA with Reverse Transcription-quantitative PCR, also investigated gene sequence using SSCP and/or PCR-sequencing. Clinical relevance changes DNA was evaluated two subgroups treated either taxane/platinum (TP,...
Targeted therapy of non-small cell lung cancer necessitates fast and reliable molecular evaluation tissue/cytologic samples within the routine diagnostic process. Here we present dynamic development EGFR mutation screening program for NSCLC patients in Poland previous 4 years. In total, 177 were analysed mutations 2011 (11,3% positive), 915 (8,7%) 2012, 1843 (9,9%, 1,2% unsuitable diagnostics) 2013, 3405 (10,6%, 1,7% 2014. Most adenocarcinomas (82,1% 93,2% 2014), number NOS materials fell...
<div>Abstract<p>Survival in epithelial ovarian cancer (EOC) is influenced by the host immune response, yet key genetic determinants of inflammation and immunity that affect prognosis are not known. The nuclear factor-κB (NF-κB) transcription factor family plays an important role many inflammatory responses, including response to cancer. We studied common inherited variation 210 genes NF-κB 10,084 patients with invasive EOC (5,248 high-grade serous, 1,452 endometrioid, 795 clear...